我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

雪松不同部位中原花青素的含量测定及其清除DPPH自由基的活性(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2015年06期
页码:
930-934
栏目:
研究论文
出版日期:
2015-11-20

文章信息/Info

Title:
Determination of proanthocyanidins and DPPH radical scavenging activity of different parts from Cedrus deodara
文章编号:
1000-3142(2015)06-0930-05
作者:
沈 薇1 石晓峰1* 宁红霞2 胡彩香1
1. 甘肃省医学科学研究院, 兰州 730050; 2. 甘肃中医学院, 兰州 730000
Author(s):
SHEN Wei1 SHI Xiao-Feng1* NING Hong-Xia2 HU Cai-Xiang1
1. Gansu Academy of Medical Science, Lanzhou 730050, China; 2. Gansu College of TCM, Lanzhou 730000, China
关键词:
雪松 原花青素 含量测定 紫外-可见分光光度法 DPPH自由基
Keywords:
Cedrus deodara proanthocyanidin content determination ultraviolet-visible spectrophotometer DPPH free radical
分类号:
R931.6, Q946
DOI:
10.11931/guihaia.gxzw201405031
文献标识码:
A
摘要:
雪松(Cedrus deodara)是松科(Pianaceae)雪松属(Cedrus)树种的泛称,为广泛分布于喜马拉雅山脉的常绿树种,包括雪松(C. deodara)、黎巴嫩雪松(C. libani)、短叶雪松(C. brevifolia)和北非雪松(C. atlantica)等。雪松的针叶药用历史悠久,主要化学成分为黄酮类、苯丙素类、有机酸类、三萜类、甾体类、多糖及针叶胶等,具有抗肿瘤、抗氧化、改善记忆、抗菌及抗病毒等多种功效。为了测定雪松不同部位中原花青素的含量,并考察其清除DPPH自由基活性的能力,该研究以Fe3+ 离子为催化剂,用正丁醇-盐酸法分别测定雪松松针、花序、小枝、木质部、树皮中原花青素的含量,并对不同部位提取液清除DPPH自由基的能力进行测定。结果表明:松针、花序、木质部、树皮、小枝中原花青素的含量分别为12.93%、12.93%、6.41%、12.00%、3.37%,在质量浓度(以生药计)为0.8 mg·mL-1时,其对DPPH自由基的清除率均达到或接近90%。该研究结果提示雪松各个部位的原花青素含量丰富,其提取液的体外抗氧化活性很高,这为雪松的进一步开发利用提供了很好的参考价值。
Abstract:
Cedrus deodara(Pinaceae family), which consists of C. deodara, C. libani, C. brevifolia and C. atlantica, is an evergreen tree growing extensively on the slopes of the Himalayas. Its leaves was a kind of chinese medicine for a long history. Its main chemical constituents include flavonoids, phenylpropanoids, organicacid,triterpenes,steroids,polysaccharides leaf glue and so on. The major pharmacological activities of pine needles of C. deodara include antitumor, antioxidation, improving memory, antibacterial and antivirus, ect. In order to determine proanthocyanidin content and DPPH radical scavenging activity of different parts from C. deodara. In this study, Fe3+ ion was used for catalysator, n-BuOH:HCl(95:5)was chromogenic agent and the detection wave-lengths were at 550 nm. And the content of proanthocyanidin was determined in different parts from C. deodara by ultraviolet-visible spectrophotometer. And antioxidant activity of extract from different parts of C. deodara were investigated by scavenging 1,1-Diphenyl-2-picrylhydrazyl free radical(DPPH). The results showed that proanthocyanidins content in different parts of C. deodara was pine needles(12.93%),inflorescence(12.93%),bark(12.00%), wood(6.41%), tender branchlets(3.37%). The extract from different parts of C. deodara had good antioxidant activity. In a certain concentration scope, there was a strong positive correlation between the scavenging activity and the concentrations. The order of IC50 was tender branchlets > pine needles > wood> bark > inflorescence. Except for branchlets, their DPPH scavenging activities were around 90% while the concentration of the extract from different parts was 0.8 mg·mL-1. The results indicated that there were higher proanthocyanidins content and antioxidant activities in different parts of C. deodara, which provide the reference on comprehensive development and utilization of C. deodara.

参考文献/References

Cui XX(崔晓霞),Zhang XL(张小丽),Luo SQ(罗书勤). 2012. Extracting process of proanthocy anidins from pinus koraiensis bark(落叶松树皮原花青素提取工艺的研究)[J]. Nat Prod Res Dev (天然产物研究与开发), 24(S1):127-130
Guan Q(
官清),Zang H(张珩),Shi XL(石晓琳). 2012. Research progress of pharmaceutical proanthocyanidin(原花青素药用研究进展)[J]. Chin J Clin Rat Drug Use(临床合理用药), 5(2A):174-175
Jia DY(
贾冬英),Cao DD(曹冬冬),Yao K(姚开). 2007. DPPH radical scavenging activity of eleocharis tuberosa peel extract(荸荠皮提取物对DPPH自由基清除活性)[J]. Nat Prod Res Dev (天然产物研究与开发), 19(5): 745-747
Li D(
李丹),Tian Y(田莹). 2010. Study on antioxidation action of grape juice(葡萄汁抗氧化作用的研究)[J]. J Anhui Agric Sci(安徽农业科学), 38(3): 1 429-1 431
Liu Y(
刘晔),Luo SQ(罗书勤),Wang BC(王博超), et al. 2011. Study on extraction technique of larch bark proanthocyanidins using ultrasonic wave(超声辅助提取落叶松树皮原花青素的工艺研究)[J]. Mod Chem Ind (现代化工), 31(1):250-252
Zhang JM(
张军民),Shi XF(石晓峰),Fan B(范彬). 2009. Chemical constituents and pharmacological activities from pine needles of cedrus trew( 雪松的化学成分及药理活性研究进展)[J]. Chin Trad Pat Med(中成药),31( 6): 928
Zhang XJ(
张小军),Xia CT(夏春镗),Wu JM(吴建铭), et al. 2009. Resource studies of proanthocyanidins(原花青素的资源研究)[J]. Chin Med Mat(中药材), 32(6):1 154-1 158
Zhang Y(
张妍),Wu XX(吴秀香). 2011. Advances in research of the proanthocyanidin(原花青素研究进展)[J]. Chin Mat Med Pharm Clin(中药药理与临床),27(6):112-115
water use efficiency of vegetable amaranth(
Amaranthus spp.)in response to drought stress[J]. Sci Hortic,102(1): 15-27
Merlo L,Passera C.
1991. Changes in carbohydrate and enzyme levels during development of leaves of Prunus persica,a sorbitol synthesizing species[J]. Physiol Planta,83:621-626
Miron D,Schaffer AA.
1991. Sucrose phosphate synthase,sucrose synthase,and acid invertase activities in developing tomato fruit of Lycopersicon escufenfum Mill and the sucrose accumulating L. hirsutum Humb. and Bonpl. [J]. Plant Physiol,95: 623-627
Paez A,Gebre GM,Gonzalez ME,
et al. 2000. Growth,soluble carbohydrates,and aloin concentration of Aloe vera plants exposed to three irradiance levels [J]. Environm Exp Bot,44(2): 133-139
Qi X(
齐欣),Cao KF(曹坤芳),Feng YL(冯玉龙). 2004. Photosynthetic acclimation to different growth light environments in seedlings of three tropical rainforest Syzycium species(热带雨林蒲桃属3个树种的幼苗光合作用对生长光强的适应)[J]. Acta Phytoecol Sin(植物生态学报),28(1): 31-38
Souza A,Paula AD,Figueiredo-Ribeiro RC.
2004. Effects of irradiance on non-structural carbohydrates,growth,and hypoglycemic activity of Rhynchelytrum repens(Willd.)C.E. Hubb.(Poaceae)[J]. Braz J Biol,64(3B): 697-706
Wang BW(
王博文),Wang Y(王洋),Yan XF(阎秀峰). 2006. Effect of light intensity on camptothecin content and a llocation in Camptotheca acuminata seedlings(光强对喜树幼苗喜树碱含量及分配的影响)[J]. J Nat Sci Heilongjiang Univ(黑龙江大学自然科学学报),23(2): 260-264
Wang Y(
王洋),Dai SJ(戴绍军),Yan XF(阎秀峰). 2004. Effects of light intensity on secondary metabolite camptothecin production in leaves of Camptotheca acuminata seedlings(光强对喜树幼苗叶片次生代谢产物喜树碱的影响)[J]. Acta Ecol Sin(生态学报),24(6): 1 118-1 122
Xiang Q(
向秋),Lei X(雷迅),Huang LZ(黄岚珍). 2009. Study of metabolic conversion of mogrol glycosides in fruit of Siraitia Grosvenorii(罗汉果皂甙类成分代谢转化规律分析)[J]. Biotechnology(生物技术),19(4): 49-51
Xu XH(
许翔鸿),Wang ZT(王峥涛),Yu GD(余国奠). 2004. Preliminary study on effects of light on plant development and alkaloid accumulation of Corydalis rhizome(光照对延胡索生长及生物碱积累影响的初步研究)[J]. J Chin Med Mat(中药材),27(11): 804-805
Yan XF(
阎秀峰),Wang Y(王洋),Guo SL(郭盛磊),et al. 2004. Seasonal variations in biomass and salidroside content in roots of Rhodiola sachalinensisas affected by gauze and red film shading(遮荫和红膜处理对高山红景天根生物量及红景天苷含量季节变化的影响)[J]. Chin J Appl Ecol(应用生态学报),(15): 382-386
Yan XF(
阎秀峰),Wang Y(王洋),Li YM(李一蒙). 2007. Plant secondary metabolism and its response to environment(植物次生代谢及其与环境的关系)[J]. Acta Ecol Sin(生态学报),27(6): 2 554-2 562
Zhong MC(
钟名诚),Li Q(李琦),Xiao C(肖聪).2010. Study on quality standard for fructus Momordicae(罗汉果的质量标准)[J]. Chin Pharm(中国药师),13(5):654-656
Zhu YJ,Komor E,Moore PH.
1997. Sucrose accumulation in the sugarcane stem is regulated by the difference between the activities of soluble acid invertase and sucrose phosphate synthase [J]. Plant Physiol,(中国药师),13(5):654-656
Zhu YJ,Komor E,Moore PH.
1997. Sucrose accumulation in the sugarcane stem is regulated by the difference between the activities of soluble acid invertase and sucrose phosphate synthase [J]. (2): 609-616

备注/Memo

备注/Memo:
收稿日期: 2014-09-21修回日期: 2014-12-01
基金项目: 甘肃省普通中医药科研项目(GZK-2012-29); 甘肃省科技支撑计划项目(1204FKCA152)。
作者简介: 沈薇(1977-),女,甘肃省酒泉市人,副研究员,研究方向为中药化学及中药质量标准,(E-mail)shenwei_0124@sina.com。 *通讯作者: 石晓峰,主任药师,教授,硕士生导师,研究方向为天然药物化学,(E-mail)shixiaofeng2005@sina.com。
更新日期/Last Update: 2015-11-20