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锰胁迫对甘蔗幼苗缺铁和失绿的影响(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2015年06期
页码:
863-867
栏目:
研究论文
出版日期:
2015-11-20

文章信息/Info

Title:
Impact of manganese stress on chlorosis and iron-deficiency in sugarcane seedlings
文章编号:
1000-3142(2015)06-0863-05
作者:
吴 星1 黄渝岚1 杨 曙1 赵尊康1 黎晓峰12*
1. 广西大学 农学院, 南宁 530005; 2. 亚热带生物资源保护与利用国家重点实验室, 南宁 530005
Author(s):
WU Xing1 HUANG Yu-Lan1 YANG Shu1ZHAO Zun-Kang1 LI Xiao-Feng12*
1. Agriculture College, Guangxi University, Nanning 530005, China; 2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning 530005, China
关键词:
甘蔗 失绿
Keywords:
sugarcane chlorosis manganese iron
分类号:
Q945.12
DOI:
10.11931/guihaia.gxzw201406027
文献标识码:
A
摘要:
近年来,甘蔗主产区的甘蔗幼苗出现严重的缺铁失绿问题,影响了我国甘蔗生产及食糖安全。为了揭示锰诱导甘蔗幼苗缺铁失绿机制,该研究采用水培试验法,对过多锰诱导的甘蔗幼叶失绿及其与铁素营养的关系进行了探讨。结果表明:过多锰胁迫下随着甘蔗中锰含量的增加,幼叶明显失绿。250、500、750 μmol·L-1处理10 d后,幼叶中的叶绿素含量分别从对照处理的1.71 mg·g-1 FW下降至0.86、0.85、0.64 mg·g-1 FW。过多锰抑制甘蔗对铁的吸收,每株植株对铁吸收量(3.22~4.40 mg)显著减少。幼叶中铁含量(116.8~128.6 mg·kg-1 DW)也随着锰处理浓度的增加而显著降低。250~750 μmol·L-1处理的甘蔗幼叶中铁的含量仅相当于对照处理的89.4%~81.2%。相反,锰处理后根和茎中铁的含量却显著增加。锰胁迫下幼叶中活性铁含量和活性铁与全铁比值(0.14~0.21)也显著降低。高锰胁迫下,幼叶中的活性铁含量(4.1~6.9 mg·kg-1 FW)相当于对照处理的25.5%~55.2%。相关分析结果显示,锰胁迫下的甘蔗叶片中活性铁含量与叶绿素含量呈显著的正相关; 锰处理后幼叶中活性铁与锰含量的比值从对照的0.71下降至0.04~0.01。这说明过多的锰可诱导甘蔗幼叶失绿,而失绿与过多的锰胁迫下甘蔗对铁的吸收、运输受阻及铁的钝化有关。
Abstract:
The main sugarcane production areas of China have been suffered from sugarcane seedlings chlorosis for several years, which affected sugarcane production and threatened sugar security of China. Manganese(Mn)-induced chlorosis and its relationship to iron nutrition in sugarcane seedlings were investigated by hydroponic experiments to elucidate mechanisms for the chlorosis in plants. The results indicated that a significant chlorosis symptom was exhibited on young leaves of sugarcane seedlingswith the increase of Mn content in sugarcane under excessive Mn stress. Chlorophyll content in young leaves decreased from 1.71 mg·g-1 FW(control)to 0.86, 0.85, 0.64 mg·g-1 FW, respectively, after the sugarcane seedlings were exposed to 250, 500 or 750 μmol·L-1 Mn solution for 10 d. Excess Mn inhibited iron(Fe)acquisition by sugarcane seedlings, which resulted in significant decrease in Fe acquisition rate(3.22-4.40 mg per plant). Fe content(116.8-128.6 mg·kg-1 DW)in young leaves decreased significantly with the increase of Mn-treatment concentration. After the treatment with 250-750 μmol·L-1Mn, Fe content in the leaves was equal to 89.4%-81.2% of that in control. On the contrary, excess Mn resulted in significant increase in Fe content in roots and stems. Moreover, both active iron content [Fe(H)] and the ratio of Fe(H)to total Fe(0.14-0.21)in young leaves under Mn stress declined significantly. Active iron content [Fe(H), 4.1-6.9 mg·kg-1 FW] in the leaves was equated to only 25.5%-55.2% of that in control. The results of correlation analysis showed that chlorophyll content was positive related to Fe(H)in the leaves under Mn stress. On the other hand, the ratio of Fe(H)to total Mn in young leaves after the treatment with Mn declined to 0.04-0.01 while that in control was 0.71. These results indicated that excessive Mn could induce the chlorosis in sugarcane seedlings, and the inhibition of acquisition, transportation and activation of Fe by Mn are involved in the induction of the chlorosis.

参考文献/References

Alam S, Kamei S, Kawai S. 2001. Amelioration of manganese toxicity in barley with iron[J]. J Plant Nutr, 24(9): 1 421-1 433
Chen ZJ(陈志坚), Yan W(严炜), Sun LL(孙丽莉), et al. 2011. Effects of manganese toxicity on plant growth, acquisition of iron and magnesium in Stylosanthes guianensis seedling(锰毒对柱花草苗期生长及铁镁吸收的影响)[J]. Chin J Trop Crops, 32(1):142-146
Führs H, Behrens C, Gallien S, et al. 2010. Physiological and proteomic characterization of manganese sensitivity and tolerance in rice(Oryza sativa)in comparison with barley(Hordeum vulgare)[J]. Ann Bot, 105: 1 129-1 140
He YQ(何勇强), Lu SN(陆申年), Li GB(黎耿碧). 1996. The manganese toxicity to pineapple in lateritic red earth from deserted manganese mine district in pot experiment(废矿还耕锰矿区土壤上菠萝锰毒害的研究)[J]. Agro Environ Prot, 15(1): 15-18
Li XF(黎晓峰), Gu MH(顾明华), Bai HY(自厚义), et al. 1996. Studies on the relationship between iron nutrition and manganese toxicity in paddy rice(水稻锰毒与铁素营养关系的研究)[J]. J Guangxi Agric Univ, 15(3):190-194
Li YR(李杨瑞), Mao LX(毛吕祥), Tang QZ(唐其展), et al. 2010. Summing up the proceedings of international workshop on declining cane sugar production in Asian countries 2008-2009:reasons and remedies(“2008-2009亚洲国家蔗糖产量降低的原因及应对措施”国际学术研讨会综述)[J]. Guangxi Agric Sci, 41(1): 80-84
Long GX(龙光霞), Huang YL(黄渝岚), He H(何红 ), et al. 2011. Characterizing nutrient status of soils causing chlorosis in sugarcane seedlings(甘蔗幼苗失绿的土壤营养特性初探)[J]. J S Agric Sci, 42(8):931-934
Tang SR(唐仕云), Tan YM(谭裕模), Yang RZ(杨荣仲), et al. 2009. Effect of trace element fertilizer on yellow and white seedling of ratoon cane(喷施微肥对宿根甘蔗黄白苗的影响)[J]. J Anhui Agric Sci, 37(30):14 648-14 649
Wen JC(文建成),Tang L(汤利),Tan XL(谭学林), et al. 2010. Variation of Fe and Zn concentrations in japonica rice grown at varied environments and different levels of fertilizer application(种植环境和施氮水平影响粳稻稻米铁、锌矿质元素含量)[J]. Crops, 1: 61-65
Wu KC(吴凯朝), Liang J(梁俊),Wei LP(韦莉萍), et al. 2011. Effects of nitrogen fixing bacteria on photosynthesis and chlorophyll fluorescence in sugarcane at elongating stage(内生固氮菌对甘蔗伸长期光合生理特性的影响)[J]. Guihaia , 31(5): 668-673
Yan W(严炜), Liu GD(刘国道), Tian J(田江). 2010. Effects of manganese availability on acquisition and distribution of manganese, iron and phosphorus in soybean Glycine max (L. )Merr.(锰有效性对大豆锰、铁和磷吸收及其分布的影响)[J]. Plant Physiol Comm, 46(9): 923-927
Yin W(尹文彦), Yao YA(姚银安). 2011. Effects of Mn toxicity on contents of mineral elements in grape plants(锰毒害对葡萄植株矿质元素含量的影响)[J]. Guizhou Agric Sci, 39(7): 213-215
Zang XP(臧小平). 1999. Manganese in soils and manganese toxicity to plants(土壤锰毒与植物锰的毒害)[J]. Chin J Soil Sci, 30(3): 139-141
Zhang XK(张西科),Zhang FS(张福锁),Mao DR(毛达如). 1994. Reasearch progress of manganese toxicity in plants(植物锰中毒研究进展)[J]. Prog Soil Sci(土壤学进展),20(5):13-21
Zeng Q(曾琦), Geng MJ(耿明建), Zhang ZJ(张志江), et al. 2004. Effects of Mn toxicity on the content of Mn, Ca, Fe and the activities of POD and CAT in Brassica napus at Seedling Stage(锰毒害对油菜苗期Mn、Ca、Fe含量及POD、CAT活性的影响)[J]. J Huazhong Agric Univ, 23(3): 300-303

备注/Memo

备注/Memo:
收稿日期: 2014-10-22修回日期: 2015-01-27
基金项目: 国家自然科学基金(312604972013); 广西自然科学基金重点项目(2012GXNSFDA053010)。
作者简介: 吴星(1985-),男,安徽萧县人,硕士研究生,研究方向为植物营养生理及调控,(E-mail)659386789@qq.com。 *通讯作者: 黎晓峰,博士,教授,主要从事植物逆境生理及植物营养学研究,(E-mail)lxf@.gxu.edu.cn。
更新日期/Last Update: 2015-11-20