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酶水解百分之五十罗汉果皂苷V方法学研究(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2015年06期
页码:
812-816
栏目:
罗汉果专题论文
出版日期:
2015-11-20

文章信息/Info

Title:
Methodology of enzymic hydrolysis of fifty percent mogroside V
文章编号:
1000-3142(2015)06-0812-05
作者:
羊学荣12 卢凤来2 王 磊2 陈 兵12 徐 风3 蔡少青3 李典鹏2*
1. 广西中医药大学 药学院, 南宁 530001; 2. 广西植物功能物质研究与利用重点实验室, 广西植物研究所, 广西 桂林 541006; 3. 北京大学 药学院, 北京 100191
Author(s):
YANG Xue-Rong12 LU Feng-Lai2 WANG Lei2 CHEN Bing12 XU Feng3 CAI Shao-Qing3 LI Dian-Peng2*
1. School of Pharmaceutical Sciences, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China; 2. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guilin 541006, China; 3. School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
关键词:
酶水解 罗汉果皂苷IIIE 罗汉果皂苷III 罗汉果皂苷IVE 赛门苷I 方法学
Keywords:
enzymic hydrolysis mogroside IIIE mogroside III mogroside IVE siamenoside I methodology
分类号:
Q946.5
DOI:
10.11931/guihaia.gxzw201407005
文献标识码:
A
摘要:
罗汉果是我国常用的一种中药,用于治疗干咳、口腔溃疡及便秘等,罗汉果皂苷是罗汉果果实中的主要药效物质,为一类葫芦烷三萜皂苷类化合物。由于个别罗汉果低糖皂苷在罗汉果中含量较低,因而难以对这些皂苷有效地进一步进行代谢或药理等研究。该研究采用RP-HPLC监测50%罗汉果皂苷V的水解过程,并对其水解条件进行正交实验优化其组合,建立了酶水解50%罗汉果皂苷V以获取罗汉果次级皂苷的方法。结果表明:酶水解50%罗汉果皂苷V获得罗汉果皂苷IIIE、III、IVE和赛门苷I等次级皂苷的最佳水解条件是pH值为5.6的乙酸-乙酸钠缓冲体系,反应温度为55 ℃,β-葡聚糖酶活性浓度为40万U·L-1,罗汉果皂苷V的反应浓度为0.2 g·L-1,水解时间为10 h。经多次验证,该水解条件重现性好,可用于从50%罗汉果皂苷V中水解制备罗汉果皂苷IIIE、III、IVE和赛门苷I等罗汉果皂苷。该研究结果为进一步水解其他罗汉果次级皂苷提供了依据。
Abstract:
Luo Han Guo, the dried fruits of Siraitia grosvenorii, is a kind of traditional Chinese medicine and used for the treatment of dry cough, sore throat, and constipation etc. Previous chemical investigations revealed that the major valid compounds of S. grosvenorii were mogrosides, belonging to cucurbitane-type triterpenes. Due to the fact that some mogrosides are low content in S. grosvenorii,it is hard to achieve enough amount of these compounds to further study their metabolism and pharmacology etc. So a methodology was established in this paper to obtain enough mogrosides of low content(such as mogroside IIIE, III, IVE, and siamenoside I)by enzymic hydrolysis of 50% mogroside V. The hydrolysis was monitored by RP-HPLC and orthogonal experiments was applied to optimize the reaction conditions. The experiment result indicated the optimal reaction conditions were: in pH 5.6 acetic acid-sodium acetate buffer solution, reaction temperature was 55 ℃, β-dextranase concentration was 400 kU·L-1, reaction concentration of mogroside V was 0.2 g·L-1, reaction time was 10 h. It had been verified that the method was repeatable, which could be applied to hydrolyze 50% mogroside V to obtain mogroside IIIE, III, IVE, and siamenoside I. This result also could be a guide for hydrolyzing others secondary-mogrosides.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期: 2014-10-03修回日期: 2015-04-06
基金项目: 国家自然科学基金(81160392,81160518); 国家科技支撑计划项目(2011BAI01B03); 桂林市科学研究与技术开发项目(20120118-6)。
作者简介: 羊学荣(1990-),男,海南儋州市人,硕士研究生,主要从事天然药物资源开发与利用研究,(E-mail)yxrxl@sina.cn。 *通讯作者: 李典鹏,博士,研究员,主要从事药物化学成分研究,(E-mail)ldp@gxib.cn。
更新日期/Last Update: 2015-11-20