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枯草芽孢杆菌肥在罗汉果上应用的效应分析(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2015年06期
页码:
807-811
栏目:
罗汉果专题论文
出版日期:
2015-11-20

文章信息/Info

Title:
Effects of the bio-fertilizer of Bacillus subtilis on the application of Siraitia grosvenorii
文章编号:
1000-3142(2015)06-0807-05
作者:
冯世鑫1 莫长明1 唐 其1 潘丽梅1 白隆华1 马小军2*
1. 广西药用植物园, 南宁 530023; 2. 中国医学科学院 药用植物研究所, 北京100093
Author(s):
FENG Shi-Xin1 MO Chang-Ming1 TANG Qi1 PAN Li-Mei1 BAI Long-Hua1 MA Xiao-Jun2*
1. Guangxi Branch Garden of Medicinal Plant, Nanning 530023, China; 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100093, China
关键词:
罗汉果 枯草芽孢杆菌 土壤微生物数量 产量 品质
Keywords:
Siraitia grosvenorii Bacillus subtilis fertilizer soil microbial yield quality
分类号:
Q949.9
DOI:
10.11931/guihaia.gxzw201405046
文献标识码:
A
摘要:
以罗汉果永青2号品种为材料,在连作6 a罗汉果的地块上,用地膜覆盖和区间隔离的方法,施入不同浓度的枯草芽孢杆菌肥,观察土壤微生物数量、罗汉果叶绿素量、白绢病发生率、产量及皂苷Ⅴ的变化,研究枯草芽孢杆菌肥对多年种植罗汉果地块的土壤微生物,植株生长和品质的影响。结果表明:枯草芽孢杆菌肥明显提高0~20 cm土壤微生物的数量,尤其是细菌数量和放线菌明显高于对照; 而引发植株发病的真菌数量显著减少,最多能减少13.8%。提高罗汉果植株叶片叶绿素含量,促进叶绿素a、叶绿素b的形成,有利光合作用积累,同时,能增加叶绿素c含量,提高植株抗逆性; 能抑制或降低白绢病病菌感染,减少白绢病发生。罗汉果果实产量得到明显提高,最大提高17.5%,同时,大中果比率提高7.5%,优化了罗汉果商品的物理性状; 能改善罗汉果品质,提高其内含物的含量,果实中的主效成份皂苷Ⅴ含量达到1.33%,显著高于对照。该研究结果为罗汉果产区使用枯草芽孢杆菌肥连续种植罗汉果提供了依据。
Abstract:
To explore the effects of bio-fertilizer of Bacillus subtilis on its soil microbial growth and quality, we investigated the soil microbial number, chlorophyll content, incidence of southern blight, production and the change of mogroside V in the fruits after treating in different concertrations of bio-fertilizer of B. subtilis, in six years of continuous cropping fields, With film-mulching and zone-isolation methods, using Siraitia grosvenorii Yongqing-2 varieties as an experimental materials. The results showed that microbial number significantly improved in the soil below 0-20 cm of the surface after applying the bio-fertilizer of Bacillus subtilis. The number of bacteria and actinomycetes significantly increased, while Fungi that caused plant disease significantly reduced to 86.2. Chlorophyll a and chlorophyll b synthesis was promoted and the accumulation of photosynthesis products increased, while the content of chlorophyll c increased improving plant resistance. The ratio of southern blight decreased while fruit production increased by 17.5%. Fruit level increased up to 7.5% improving for better sale. The inherent quality sublimated that main-effect components mogrosides V reached 1.33%. This would provide evidence that Siraitia grosvenorii growing areas can use bio-fertilizer of Bacillus subtilis to increase the yield and quality.

参考文献/References

Agriculture Industry Standards of People’s Republic of China(中华人民共和国农业行业标准). 2003. Siraitia grosvenorii(罗汉果)[S]. 2(NY/T 694-2003 ) Cai XC(蔡学清),He H(何红),Lin CP(林彩萍), et al. 2005. Effects of endophytic bacteri al strai n Bs-2 on rice seedi ng growth(内生枯草芽孢杆菌Bs-2对水稻苗生长效应)[J]. J Fujian Agric For Univ:Nat Sci Ed(福建农林大学学报·自然科学版),34(2):189-194
Ghosh PK,Ajay,Bandyopadhyay KK,et al. 2004.Comparative effectiveness of cattle manure,poultry manure,phosphoeompost and fertilizer-NPK on three cropping systems in vertisols of semi-arid tropics. Ⅱ Dry matter yield,nodulation,chlorophyll content and enzyme activity[J]. Biores Technol,(95):85-93
Hong YC(洪永聪),Lai YB(来玉宾),Ye WN(叶雯娜),et al. 2006. Mechanism of the control of tea grey blight disease by bacillus subtilis strain TL2(枯草芽孢杆菌株TL2对茶叶轮纹斑病的机制)[J]. J Tea Sci(茶叶科学),26(4)259-264
Jia Z,Yang X. 2009. A minor,sweet cucurbitane glycoside from Siraitia grosvenorii [J]. Nat Prod Commun, 4(6):769-772
Lu FL(卢凤来),Li DP(李典鹏),Liu JL(刘金磊),et al. 2009. Chromatographic fingerprinting analysis on chemical compositions of Siraitia grosvenorii fruit with different drying treatments(不同干燥处理的罗汉果化学成分色谱指纹图谱分析)[J]. Guangxi Agric Sci(广西农业科学),40(6):625-628
Li DQ(李德全),Chen ZY(陈志谊),Nin YF(聂亚锋). 2008. Antifungal substances producted by a high-yielding mutant of Bs-916 and their effects inducing-resistance on rice plant(生防菌Bs-916及高效突变菌株抗菌物质及其对水稻抗性诱导作用的研究)[J]. Acta Phytopathol Sin(植物病理学报),38(2):192-198
Li HS(李合生). 2000. Plant physiological and Biochemical Principles and Techniques(植物生理生化实验原理和技术)[M]. Beijing(北京):Higher Education Press(高等教育出版社):134 Peng HM(彭浩民),Cai ZX(蔡昭雄),Liao MY(廖苠焰),et al. 2011. Integrated control of main diseases and insect pests of field investigation of Siraitia grosvenorii in Xing’an County pests(兴安县罗汉果主要病虫害田间调查及综合防治)[J]. Southern Garden(南方园艺),22(4):39-41
Salies JF,Van VJA,Vanelsas JD. 2004.Multivariate analyses of burkholderia species in soil:effect of crop and land use history[J]. Environ Microbiol,70(7):4 012-4 020
State Pharmacopoeia Commission(国家药典委员会). 2010. People’s Republic of China Pharmacopoeis(first part)(中华人民共和国药典 一部)[M]. Beijing(北京):Chinese Medical Science and Technology Press(中国医药科技出版社):197 Yang Y(杨义),Huang YL(黄永禄),Zhang XQ(张献强),et al. 2010. Control effects of Bacillus subtilis strain Bv22 on jasmine sclerotium blight(芽孢杆菌BV22菌株对茉莉花白绢病防治效果试验)[J]. Guangxi Agric Sci(广西农业科学),(6):46-48
Yang XM(杨兴明),Xu YC(徐阳春),Huang QW(黄启为),et al. 2008. Organic-like fertilizers and its relation to sustainable development of agriculture and protection of eco-environment(有机(类)肥料与农业可持续发展和生态环境保护)[J]. Acta Pedoloqica Sin(土壤学报),45(5):925-932
Zhang SS(张树生),Yang XM(杨兴明),Mao ZS(茆泽圣),et al. 2007. Effects of sterilization on growth of cucumber plants and soil microflora in a continuous mono-cropping soil(连作土灭菌对黄瓜生长和土壤微生物区系的影响)[J]. Acta Ecol Sin(生态学报), 27(5):1 809-1 817
Yu LJ(于丽静),Dong HQ(董华强). 2007. Study on new extraction method of dihydrocharcone from Lithocarpus polysachyus Pehd(从多穗柯中提取二氢查耳酮的研究)[J]. Food Ind(食品工业),(3):1-2
Yan XC(严贤春). 2003. Research to the development and utilization of natural sweeteners plant(天然甜味剂植物的开发利用研究)[J]. Res & Dev Food(食品研究与开发), 24(1):59-62
Zhang J(张俭),Zeng JY(曾军英),Tang M(唐铭). 2012. The effect of different extracts from Lithocarpus polystachyus Rehd on the activity of CAT and MDA in diabetic mice(多穗柯不同液相提取物对糖尿病小鼠 MDA/CAT活性的影响)[J]. Hubei Agric Sci(湖北农业科学), 51(2):354-357
Zhang GL(张桂玲),Wen SM(温四民). 2006. The research progress of sweet plants(甜味植物研究进展)[J]. Anhui Agric Sci(安徽农业科学), 34(18):4 712-4 713
Zhang Y(张毅),Nin ZX(宁正祥),Dong HQ(董华强). 2011. Inhibitory potential of trilobatin from Lithocarpus polystachyus Rehd against key enzymes linked to type Ⅱ diabetes and its antioxidant activity(多穗柯三叶苷的抑制糖尿病关键酶活性和抗氧化性)[J]. Food Sci(食品科学), 32(5):32-35
Zeng XY(曾祥艳),Chen JY(陈金艳),Liao JM(廖健明),et al. 2014. Effects of different planting densities on growth of young forest in Lithocarpus polystachyus Rehd(不同栽培密度对多穗柯幼林生长的影响)[J]. Nonw For Res(经济林研究), 32(1):113-116
Zeng XY(曾祥艳),Chen JY(陈金艳),Li JL(李建林),et al. 2011. The introduction study on Camellia oleifera var. nhgean(义安油茶引种初报)[J]. Guangxi For Sci(广西林业科学), 40(4):322-324

备注/Memo

备注/Memo:
收稿日期: 2014-12-05修回日期: 2015-03-27
基金项目: 国家“十二五”科技支撑计划项目(2011BA101B03); 广西农业科技成果转化项目(桂科转1123013-12); 广西自然科学基金(2012jjAA30044); 广西重大专项计划项目(桂科重1298001-2-2)。
作者简介: 冯世鑫(1966-),男(壮族),广西横县人,副研究员,从事药用植物繁育与栽培研究,(E-mail)shixin1996@126.com。 *通讯作者: 马小军,博士,研究员,研究方向为药用植物生物技术,(E-mail)xjma@public.bta.net.cn。
更新日期/Last Update: 2015-11-20