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罗汉果果肉中糖类物质组成与含量分析(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2015年06期
页码:
775-781
栏目:
罗汉果专题论文
出版日期:
2015-11-20

文章信息/Info

Title:
Determination of sugar components and contents in fruit flesh of Siraitia grosvenori
文章编号:
1000-3142(2015)06-0774-08
作者:
王海英马小军2?莫长明赵 欢涂冬萍白隆华冯世鑫
1. 天津中医药大学 中药学院, 天津 300193; 2. 中国医学科学院 中国协和医科大学 药用植物研究所, 北京 100193; 3. 广西药用植物园, 南宁 530023
Author(s):
WANG Hai-YingMA Xiao-Jun2?MO Chang-Ming ZHAO HuanTU Dong-PingBAI Long-HuaFENG Shi-Xin
1.CollegeofTraditionalChinesePharmacy,TianjinUniversityofTraditionalChineseMedicine,Tianjin300193,China;
2.Instituteof MedicinalPlantDevelopment,ChineseAcademyof MedicalSciencesandPekingUnionMedicalCollege,
Beijing100193,China;3.GuangxiBotanicalGardenof MedicinalPlant,Nanning530023,China
关键词:
罗汉果糖种类与含量干燥方式
Keywords:
Siraitia grosvenoriisugar components and contentsdrying methods
分类号:
Q945.1
DOI:
10.11931/guihaia.gxzw201405062
文献标识码:
A
摘要:
罗汉果果实中富含糖分,糖类物质的组成及其含量对果实的内在品质有重要影响,然而多年来对其品质的研究多集中在罗汉果苷上,果实中可溶性糖种类与含量迄今尚未见有系统地报道.该研究以干燥的罗汉果果实为材料,采用PMP柱前衍生化—高效液相色谱紫外检测法、高效液相色谱示差折光检测法分别检测果肉中可溶性糖的种类与含量,并进行方法学考察.结果表明:PMP柱前衍生化—高效液相色谱紫外检测法只能检出罗汉果果实中存在的2种还原性醛糖———葡萄糖、甘露糖;而高效液相色谱示差折光检测法则可一次性检出葡萄糖、果糖、蔗糖、棉籽糖、多糖5种糖分.与柱前衍生化法相比,高效液相色谱示差折光检测法更适合用来全面分析罗汉果果实中糖分的种类和含量.不同罗汉果品种果实中糖的组分一致,但含量有显著差别.另外,样品的干燥方式会影响果实中的总糖及各组分的相对含量.冻干果肉中蔗糖和葡萄糖相对含量最高,烘干则导致蔗糖和葡萄糖下降,果糖与多糖相对含量增加.
Abstract:
Components and contents of carbohydrates have important effects on fruit internal quality. Fruit of Siraitia grosvenorii is rich in sugars. However, researches on fruit quality mainly focus on mogrosides over the years,no systematic study about S. grosvenorii fruit carbohydrates and their monomer sugar constituents has been reported so far. In this study, dry fruit was selected as experimental material. PMPpre column derivatization HPLCUV detector method and HPLCrefractive index detector method were employed to determine the carbohydrates components and their contents of monomer sugar constituents in dried fruit flesh, and the methodological study was carried out. The results indicated only two kinds of reducing aldosesglucose and mannose could be detected by PMPpre column derivatization HPLCUV detector method, while five kinds of carbohydrate: glucose, fructose, sucrose, raffinose and polysaccharide could be detected simultaneously by HPLCrefractive index detector method. As compared with PMPpre column derivatization HPLCUV detector method, HPLCrefractive index detector method was much more suitable for the general analysis of components and contents of carbohydrate in fruits of S. grosvenorii. Sugar components were the same while concentrations of each monomer sugar constituents were significantly different between different S. grosvenorii varieties. On the other hand, drying methods had obvious effects on the total sugars content as well as the relative contents of 5 kinds of sugar components. The relative contents of sucrose and glucose in freeze dried fruit flesh were much higher than those other 3 kinds of sugars. However, high temperature drying treatment resulted in significant decrease in sucrose and glucose concentrations, the relative contents of fructose and polysaccharide became the most.

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备注/Memo

备注/Memo:
收稿日期: 2014-12-19 修回日期: 2015-03-26
基金项目: 国家自然科学基金(30860379);国家科技支撑计划项目(2011BA101B03);中国医学科学院药用植物研究所创新团队发展计划(121306)。
作者简介: 王海英(1972-),女,河北唐山人,博士,副教授,主要从事药用植物生理与分子生物学研究,(E-mail)haiyingwang-99@163.com。 *通讯作者: 马小军,博士,研究员,研究方向为药用植物生物技术,(E-mail)mayixuan10@163.com。
更新日期/Last Update: 2015-11-20