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|本期目录/Table of Contents|

睫毛萼凤仙花的组织培养及其愈伤组织的超微观察

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2011年02期
页码:
262-265
栏目:
植物生理学与分子生物学
出版日期:
2011-04-21

文章信息/Info

Title:
Tissue culture of Impatiens blepharosepalaand ultrastructural observation of callus
作者:
王雪娟1 程新利2 柴瑞娟3
1.安徽科技学院 城建与环境学院, 安徽 凤阳 233100; 2.天津市园林开发建设有限公司, 天津 300203; 3.安徽工程科技学院 生化工程系, 安徽 芜湖 241000
Author(s):
WANG Xue-Juan1 CHENG Xin-Li2 CHAI Rui-Juan3
1.College of Urban Construction and Environment, Anhui Science and Technology University, Fengyang 233100, China; 2.Landscape development and construction of tianjin co.Ltd, Tianjin 300203, China; 3.Department of Bioscience and Engneering, Anhui Technology and Science College, Wuhu 241000, China
关键词:
睫毛萼凤仙花 离体培养 超微观察
Keywords:
Impatiens blepharosepala culture in vitro ultrastructural observation
分类号:
-
DOI:
-
文献标识码:
A
摘要:
试验以睫毛萼凤仙花无菌苗的叶片、茎和根为外植体进行离体培养,并对两种具有不同再分化能力的愈伤组织进行电镜超微观察。结果表明:在培养基MS+NAA 0.5 mg/L+6BA 0.5 mg/L上愈伤组织诱导率达最高,叶片愈伤组织的诱导率为100%,茎的愈伤组织诱导率为67%,而根却没有愈伤组织的发生;叶〖JP2〗片愈伤组织在MS+NAA 0.5 mg/L+6BA 2.0 mg/L上培养40 d后有不定芽的发生,而茎的愈伤组织却没有器官发生;电镜观察表明,具有再分化能力的愈伤组织的细胞排列〖JP〗不规则,其表面有较明显的类似组织分化。
Abstract:
Leaves,stem and root of Impatiens blepharosepala seedling were cultured in vitro,and two types of callus having different differentiation were observed by electron microscope. The results showed that the induction rate was highest on MS+NAA 0.5 mg/L+6BA 0.5 mg/L,the induction rate of leaves was 100% and the rate of stems was 67%,but the roots can’t be induced. Callus of leaves can be induced buds on MS+NAA 0.5 mg/L+6BA 2.0 mg/L after 40 d,but there was no organ generated from stems. Cells of callus having differentiation arrayed irregularly and there was obviously similar tissue differentiation by electron microscope.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-04-25