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|本期目录/Table of Contents|

罗汉果转抗病基因NPR1的研究

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2011年02期
页码:
250-254
栏目:
植物生理学与分子生物学
出版日期:
2011-04-21

文章信息/Info

Title:
Transferring disease resistance gene NPR1 into Siraitia grosvenorii
作者:
杨华 李惠敏 覃屏生 高成伟 秦新民*
广西师范大学 生命科学学院, 广西 桂林 541004
Author(s):
YANG Hua LI Hui-Min QIN Ping-Sheng GAO Cheng-Wei QIN Xin-Min*
College of Life Science, Guangxi Normal University, Guilin 541004, China
关键词:
罗汉果 农杆菌介导 遗传转化 NPR1基因
Keywords:
Siraiti grosvenoriiAgrobacteriummediated genetic transformationNPR1 gene
分类号:
-
DOI:
-
文献标识码:
A
摘要:
以罗汉果子叶为外植体,研究了影响根癌农杆菌介导的罗汉果遗传转化的若干因素,建立了罗汉果遗传转化体系。结果表明,5 d苗龄的子叶、预培养1 d、侵染20 min、共培养4 d、共培养温度22 ℃、AS100 μmol/L、Hy浓度不定芽筛选为10 mg/L,生根筛选为20 mg/L转化率最高。抗性苗经PCR和Southern印迹分析,初步证实NPR1基因已整合至罗汉果基因组中,转化率为1.5%。
Abstract:
Using cotyledon of Siraitia grosvenorii as explants,an efficient and reliable transformation system was established. Several factors affecting genetic transformation of Agrobacteriummediated S.grosvenorii were studied. The results showed that 5 days aged cotyledon using for infection explants,preculture 1 d,bacterial infection time 20 min,co-culture 4 d with 22 ℃,AS concentrate 100 μmol/L,10 mg/L of hygromycin was suitable screening concentration for the differentiation of adventitious bud and root could get the better transformation efficiency under 20 mg/L of hygromycin. The transformants were confirmed by PCR and Southern blotting analysis with the transformation rate 1.5%.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-04-22