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|本期目录/Table of Contents|

荞麦属种质资源发芽种子酯酶同工酶研究

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2011年02期
页码:
233-238
栏目:
植物生理学与分子生物学
出版日期:
2011-04-21

文章信息/Info

Title:
Esterase isozyme on sprouting seeds of genus Fagopyrum
作者:
张以忠12 陈庆富1*
( 1.贵州师范大学 生命科学学院 植物遗传育种研究所, 贵阳 550001; 2.毕节学院 环境与生命科学系, 贵州 毕节 551700 )
Author(s):
ZHANG Yi-Zhong12 CHEN Qing-Fu1*
1.Institute of Plant Genetics and Breeding, School of Biological Technology and Engineering, Guizhou Normal University, Guiyang 550001, China; 2.Department of Environment and Life Science, Bijie College, Bijie 551700, China
关键词:
甜荞 苦荞 野荞 酯酶同工酶 系统关系
Keywords:
common buckwheat tartary buckwheat wild buckwheat esterase isozyme phylogeny
分类号:
-
DOI:
-
文献标识码:
A
摘要:
用聚丙烯酰胺凝胶电泳对荞麦属8个种(含大粒组7个和小粒组1个)33份材料发芽种子的酯酶同工酶进行了研究。结果表明:酯酶同工酶不同酶带合计22条,不同物种的酶带数4到8条。其中,甜荞有8条带,苦荞为7条。酶带及聚类分析表明,大粒组荞麦种的谱带与细野荞等小粒组荞麦种间差异极大,甜荞和苦荞酶带分别与大野荞和毛野荞相似,并分别与F.megaspartaniumF.pilus聚类最近,支持F.megaspartaniumF.pilus可能分别是甜荞和苦荞祖先种的假说。
Abstract:
The esterase isozyme of thirtythree collections of cultivated and wild buckwheat belonging to eight species of genus Fagopyrum including seven species in the bigachene group and one species in the smallachene group were studied by means of polyacrylamide gel eletrophoresis(PAGE). The results showed that there were twentytwo different bands of esterase isozyme,with a range from four bands to eight bands among different buckwheat species. F.esculentum and F.tataricum had eight bands and seven bands,respectively. The variations of esterase isozyme zymograph were much great among different buckwheat species but less in the same species. The results of the zymograph analysis and the clustering showed that there were great differences of zymographs between the bigachene group and the smallachene group and that F.megaspartanium and F.pilus were close in clustering tree to F.esculentum and F.tataricum,respectively,supporting Chen’s hypothesis that F.megaspartanium and F.pilus might be the ancestor species of F.esculentum and F.tataricum,respectively.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-04-22