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|本期目录/Table of Contents|

盐肤木基因组DNA提取方法改进及AFLP体系的建立

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2011年01期
页码:
36-38
栏目:
植物系统学与演化植物学
出版日期:
2011-01-15

文章信息/Info

Title:
A modified method of genomic DNA extraction and establishment of AFLP systems in Rhus chinensis
作者:
庞雅文1 段立柱1 宋德应2 任竹梅1*
1.山西大学 生命科学学院, 太原 030006; 2.湖北五峰土家族自治县林业局, 湖北 五峰 443400
Author(s):
PANG Ya-Wen1 DUAN Li-Zhu1 SONG De-Ying2 REN Zhu-Mei1*
1.School of Life Science, Shanxi University, Taiyuan 030006, China; 2.Bureau of Forestry of Wufeng County, Wufeng 443400, China
关键词:
盐肤木 DNA提取 4×CTAB法 AFLP技术
Keywords:
Rhus chinensis DNA extraction 4×CTAB method AFLP technology
分类号:
-
DOI:
-
文献标识码:
A
摘要:
经过反复试验,摸索出一种提取高质量植物基因组DNA的方法:改良的4×CTAB法。以盐肤木叶片为实验材料,提取到高质量的基因组DNA,建立了酶切、连接、预扩增、选择性扩增的AFLP反应体系。通过两种引物组合“E+3/M+3”和“E+2/M+3”策略筛选出8对条带分辨率高、多态性好的引物组合,优化了盐肤木的AFLP银染反应体系,得到了清晰的AFLP指纹图谱,为盐肤木种群遗传多样性研究奠定了基础。
Abstract:
A modified method(4×CTAB)was used to extract the effective genomic DNA from the leaves of Rhus chinensis,and the systems of DNA restriction reaction,ligase reaction,preamplification and selective amplification reaction were established in this study. Using the two kinds of primercombined methods (E+3/M+3 and E+2/M+3),8 pairs of primers were finally selected to amplify the genomic DNA of R.chinensis,and clear bands with high genetic polymorphism and difference were obtained. The present results provided a strong foundation for the further examination of population genetic diversity and structure of R.chinensis using AFLP method.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-04-07