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|本期目录/Table of Contents|

高效液相色谱法测定罗汉果根中两个降三萜配糖体

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2010年06期
页码:
891-894
栏目:
植物化学与化学生物学
出版日期:
2010-11-15

文章信息/Info

Title:
Determination of two nor- triterpenoid glycosides in the roots of Siraitia grosvenorii by HPLC method
作者:
卢凤来1 卢艺芳12 陈换莹12 刘金磊1 李典鹏1*
1.广西植物研究所, 广西 桂林 541006; 2.广西师范大学, 广西 桂林 541004
Author(s):
LU Feng-Lai1 LU Yi-Fang12 CHEN Huan-Ying12 LIU Jin-Lei1 LI Dian-Peng1*
1.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China; 2.Gangxi Normal University, Guilin 541004, China
关键词:
罗汉果酸苷乙Ⅱ 罗汉果酸苷甲Ⅱ HPLC 测定
Keywords:
Siraitic acid ⅡB Siraitic acid ⅡA HPLC detemination
分类号:
-
DOI:
-
文献标识码:
A
摘要:
建立了高效液相色谱测定罗汉果根中两个结构新颖的降三萜糖苷,罗汉果酸苷乙Ⅱ(Siraitic acid ⅡB)和罗汉果酸苷甲Ⅱ(Siraitic acid ⅡA)含量的方法。色谱条件:色谱柱:ZORBAX SBC18柱(4.6 mm×150 mm,5 μm);柱温:25 ℃;流动相:乙腈水(梯度洗脱:0→4 min:35%乙腈;4→16 min:35%→45%乙腈);流速:0.5 mL/min;检测波长:230 nm;进样量:10 μL。结果表明,罗汉果酸苷乙Ⅱ在0.05~0.3 mg/mL,罗汉果酸苷甲Ⅱ在0.05~0.25 mg/mL之间线性关系良好。该方法稳定,精密度、重现性好,且样品处理简单,提取与检测时间短。
Abstract:
A HPLC method was developed for determining the contents of Siraitic acid ⅡB and Siraitic acid ⅡA,which were novel 29norcucurbitane triterepene glycosides in the roots of Siraitia grosvenorii. The chromatographic analysis was carried out on a ZORBAX SBC18 column(4.6 mm×150 mm,5 μm),and the mobile phase was acetonitrile(A)water(gradient elution:0→4 min:0→35%A,4→16 min:35%→ 45%A). The flow rate was maintained at 0.5 mL/min. The detection wavelength was set at 230 nm,and the column temperature was controlled at 25 ℃. The sample injection volume was 10 μL. The results indicated that Siraitic acid ⅡB had a linear relationship at the range of 0.05-0.3 mg/mL,and Siraitic acid ⅡA had a linear relationship at the range of 0.05-0.25 mg/mL. This method was accurate,stable and reproducible,while the preparation of samples was simple and detected time was short.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-01-28