我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

广西岩溶区烟草黑胫病拮抗细菌的筛选鉴定及其抗病机理

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2010年06期
页码:
869-875
栏目:
植物生理学与分子生物学
出版日期:
2010-11-15

文章信息/Info

Title:
Screening a group of cellulose-degradation bacteria and their zymogenic characteristics
作者:
黄宁珍 区婵 何金祥* 赵志国 王新桂 郭伦发 周浩 龚庆芳
广西壮族自治区中国科学院 广西植物研究所, 广西 桂林 541006
Author(s):
HUANG Ning-Zhen OU Chan HE Jin-Xiang* ZHAO Zhi-Guo WANG Xin-Gui GUO Lun-Fa ZHOU Hao GONG Qing-Fang
Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China
关键词:
黑胫病 拮抗细菌 筛选鉴定 抗病机理
Keywords:
cellulose-degradation bacteria screening zymogenic characteristics FPase CMCase mixed fermation
分类号:
-
DOI:
-
文献标识码:
A
摘要:
用稀释法从腐殖泥中分离菌株,根据它们在羧甲基纤维素钠(CMC-Na)-刚果红培养基上的透明圈直径及对滤纸的崩解能力,获得7株纤维素分解细菌,分别编号为:B-37、B-35、B-31、B-25、B-17、Z-a和Z-b;其中菌株Z-b的纤维素崩解能力最强,分子鉴定结果表明它与Stenotrophomonas maltophilia的16S rDNA序列有99.8%的同源性,初步确定为嗜麦芽窄食单胞菌。比较7个菌株在不同C源、无机盐培养基上的生长速度发现,适合7个菌株生长的C源为马铃薯浸出液,无机盐组分为:CaCl2 0.20、MgSO4 1.25、NaCl 5.00、(NH4)2SO4 1.30、KH2PO4 1.35、FeSO4?7H2O 0.015、Na-EDTA 0.02 g/L。研究7个菌株的产酶条件发现,在滤纸为唯一碳源的培养基中,菌株Z-b的滤纸酶(FPase)和CMC酶(CMCase)活性最大,为0.099 和0.075 U/ml;而在固体PSA上,菌株B-31和B-37 的FPase 和CMCase活性最高,为0.131和0.175 U/ml。7个细菌单独发酵,Z-b对滤纸的崩解能力最强,滤纸块完全崩解成粉未状;与真菌34混合发酵,菌株组合Z-a+34、Z-b+34、B-31+34、B-25+34将滤纸完全水解为水溶性物质。可见,各菌株的纤维素酶活与培养条件密切相关;某些真、细菌之间存在协同作用,它们混合发酵可以大大提高纤维素的水解效率。
Abstract:
Phytophthora parasitica var.nicotianae and soil bacteria were isolated from Jingxi county,a karst area in southwest of Guangxi province where produced high grade tabacco. Antagonistic bacteria to P.parasitica var.nicotianae were screened out according to their antagonistic action and antidisease in vitro and field experiment. The well antagonistic effect strains were identified by combining the results of morphological observation,physiological and biochemical characteristics analyse,and 16S rDNA sequence test. And pilot study of the mechanism of antidisease of P.parasitica var.nicotianae was carried out at the same time. Results showed that a total of 340 strains of bacteria were isolated from 8 samples of soil. And three strians with well antagonistic effect which marked as 823,670,and 13 were screened out. They were the bacteria belonged to Bacillus,Lysobacter,and Pseudomonas respectively. The mechanism of the three strains against P.parasitica var.nicotianae was to excrete some protein enzymes or other substance which could dissolve the cell wall or membrane of pathogeny and killed them finally. The active substance of strain 823 and 13 were mainly protein. But for the strain 670,there were some other factors to work besides the protein compounds.

参考文献/References

-

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2011-01-28