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|本期目录/Table of Contents|

松茸组织分离物的rDNA-ITS序列鉴定

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2010年02期
页码:
181-184
栏目:
植物系统学与演化植物学
出版日期:
2010-03-15

文章信息/Info

Title:
Identification of cultures isolated from fruit body of Tricholoma matsutake by rDNA-ITS sequence analysis
作者:
张灵12赵琪1陈严平1孟珍贵1李荣春1*
1.云南农业大学 食用菌研究所, 昆明 650201; 2.云南农业大学 基础与信息工程学院, 昆明 650201
Author(s):
ZHANGLing12ZHAOQi1CHENYan-Ping1MENGZhen-Gui1LIRong-Chun1*
1.Insititution of Edible Fungi, Yunnan Agricultural University, Kunming 650201, China; 2.College of Science and Information Engineering, Yunnan Agricultural University, Kunming 650201, China
关键词:
松茸 子实体 分离物 ITS序列
Keywords:
Tricholoma matsutake fruit body mycelia rDNA-ITS
分类号:
-
DOI:
-
文献标识码:
-
摘要:
以采自云南丽江的松茸子实体为材料,进行组织分离后,利用一对ITS引物(ITS1ITS4)对子实体(SR176B,SR172B)和分离物(SR176H,SR172H)进行了PCR扩增、琼脂糖凝胶电泳分析,得到了700 bp左右的扩增条带,进一步对ITS序列进行同源性检索比对,结果表明SR176H与SR176B,SR172H与SR172B序列同源性均为100%,鉴定出该分离物就是松茸的纯培养物。
Abstract:
A pair of general primer(ITS1ITS4)was used to test in the amplification of the internal transcribed spacer(ITS)region of the chromosomal DNA of fruit body of Tricholoma matsutake of Lijiang and its isolates. The results analyzed by Agarose gel electrophoresis showed that the primers of ITS1 and ITS4 amplified about 700bp fragments for all samples. The ITS fragments of T.matsutake fruit body and its corresponding isolates were sequenced and aligned.Their sequences of SR176H and SR176B showed a 100% homogeneity,the same result also appeared between SR172H and SR172B.Based on these facts,SR176H and SR172H were confirmed to be pure cultures of T.matsutake.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2010-04-29