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|本期目录/Table of Contents|

半制备高效液相色谱制备5种苯乙醇苷类单体

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2010年02期
页码:
269-273
栏目:
植物生物化学与化学生物学
出版日期:
2010-03-15

文章信息/Info

Title:
Preparation of five phenylethanoid glycoside components by semipreparative HPLC
作者:
陈文娟12李典鹏1陈月圆1黄永林1文永新1*
广西壮族自治区中国科学院广西植物研究所, 广西 桂林 541006; 2.广西师范大学 生命科学学院, 广西 桂林 541004
Author(s):
CHENWen-Juan12LIDian-Peng1CHENYue-Yuan1HUANGYong-Lin1WENYong-Xin1*
1.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China; 2.College of Life Sciences, Guangxi Normal University, Guilin 541004, China
关键词:
半制备高效液相色谱 牛耳朵 苯乙醇苷类
Keywords:
semi-preparative HPLC Chirita eburnea phenylethanoid glycosides
分类号:
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DOI:
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文献标识码:
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摘要:
利用MCI柱层析和半制备型反相高效液相色谱法从牛耳朵中分离制备了5种苯乙醇苷类单体。在Eclipse XDBC18(9.4 mm×250 mm,5 μm)半制备柱上,以甲醇水为流动相,流速为4 mL/min,采用梯度洗脱方式,制备得到5个组分。经波谱(EI-MS,1H-NMR、13C-NMR)分析,分别鉴定为plantainoside(1)、chiritoside C(2)、plantaninoside B(3)、plantamajoside(4)和desrhamnosylverbascoside(5)。高效液相色谱分析表明所制备5种化合物纯度均高于98%。该方法分离条件简单,高效,一次可得到5个单体,目标产物纯度较高。
Abstract:
A method was established for the isolation and preparation of five phenylethanoid glycoside components from Chirita eburnea using MCI column chromatography and semipreparative HPLC. A semipreparative Eclipse XDBC18 (9.4 mm×250 mm,5 μm) was used in the preparation process. By gradient elution with methanolwater as the mobile phase at a flow rate of 4 mL/min,five phenylethanoid glycoside components were obtained from Chirita eburnea,and on the basis of spectral data (EI-MS,1H-NMR,13C-NMR),these compounds were identified as plantainoside(1),chiritoside C(2),plantaninoside B(3),plantamajoside(4) and desrhamnosylverbascoside(5),and the purities of these five phenylethanoid glycoside components analyzed by HPLC were all beyond of 98%. The method is efficient,simple with high purification and got 5 components for one time.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2010-04-29