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冰冻切片技术在海滨锦葵细胞学研究中的应用

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2010年02期
页码:
170-173
栏目:
植物系统学与演化植物学
出版日期:
2010-03-15

文章信息/Info

Title:
Cryosectioning technique for cytological studies in Kosteletzkya virginica
作者:
李贺刘松梅孙艳阮成江
大连民族学院 生物技术与资源利用国家民委教育部重点实验室, 辽宁 大连 116600
Author(s):
LIHeLIUSong-MeiSUNYanRUANCheng-Jiang
Key Laboratory of Biotechnology & BioResources Utilization, Dalian Nationalities University, Dalian116600, China
关键词:
冰冻切片技术 海滨锦葵 蔗糖保护—液氮速冻法
Keywords:
cryosectioning technique Kosteletzkya virginica sucroseprotecting and liquid nitrogen quick frozen method
分类号:
-
DOI:
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文献标识码:
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摘要:
为解决普通石蜡切片观察植物样品繁琐与耗时长的问题,利用改进的蔗糖保护—液氮速冻切片法,进行海滨锦葵不同器官细胞学研究。结果表明:(1)适合于海滨锦葵不同器官的最适蔗糖浓度不同,适于含水量较大的营养器官(根、茎和叶)的最适蔗糖浓度比含水量较小的花器官(柱头裂片、花柱、子房和花药)高。(2)利用最适蔗糖浓度的蔗糖保护-液氮速冻切片方法,获得了完整而清晰的海滨锦葵各器官组织细胞结构的切片;海滨锦葵具有典型的双子叶草本植物营养器官的组织细胞结构特征,花柱为闭合型花柱,子房为多室复子房。表明基于蔗糖保护-液氮速冻的冰冻切片技术在植物细胞学研究中具有广阔的应用前景。
Abstract:
To solve the fussy and longtime consuming problems in observing general paraffin sections of plant specimens,the method combining sucroseprotecting and liquid nitrogen frozen was used to study on cytology of different organs of Kosteletzkya virginica. The results showed:(1)It is different for the different organs of K.virginica to optimal sucrose concentration. Optimal sucrose concentrations for more water content organs(root,stem and leaf)are higher than that of less water content organs(stigma lobe,style,ovary and anther);(2)By the cryosectioning method of combining sucroseprotecting of optimal concentration with liquid nitrogen quick frozen,the integral and clear cryosections were obtained,which could discriminate from organizational and cellular structures of different organs in K.virginica. This indicated K.virginica has typical structure characteristics of tissue cell of vegetative organs in dicotyledonous herbaceous plants. Its style belongs to solid style and ovary is multilocular ovaries. Cryosectioning technique combining sucroseprotecting and liquid nitrogen quick frozen is useful for investigating plant cytology.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2010-04-29