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|本期目录/Table of Contents|

黄瓜无菌苗雌花诱导体系的建立

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2010年02期
页码:
242-245
栏目:
植物生理学与分子生物学
出版日期:
2010-03-15

文章信息/Info

Title:
Establishment for induction system of female flowers in steriled seedlings of Cucumis sativus
作者:
黄作喜12王芳1邱超1陈熠1田生辉1徐海英1
1.内江师范学院“特色农业资源研究与利用”高校重点实验室, 四川 内江 641112; 2.内江师范学院 花卉研究所, 四川 内江 641112
Author(s):
HUANGZuo-Xi12WANGFang1QIUChao1CHENYi1TIANSheng-Hui1XUHai-Yin1
1.Key Laboratory of Colleges and Universities for Research and Utilization of Distinctive agricultural Undertakings, Neijiang Normal University, Neijiang 641112, China; 2.Flower Research Institute of Neijiang Teachers College, Neijiang 641112, China
关键词:
黄瓜 无菌苗 雌花 诱导体系
Keywords:
Cucumis sativus steriled seedlings female flowers induction system
分类号:
-
DOI:
-
文献标识码:
-
摘要:
研究Spm和IAA对无菌黄瓜苗雌花诱导的协同作用,及不同外植体、培养基中琼脂含量和KH2PO4含量对雌花诱导的影响,由此建立了有效的雌花诱导体系。黄瓜去根苗接种在MS培养基上,单独添加Spm、IAA时的雌花诱导率、雌花数偏低或为0,同时添加12 mg?L-1 Spm与0.01 mg?L-1 IAA时达21%、29枚,对照组未见雌花,说明Spm和IAA对雌花诱导的协同作用显著。实验证明,在全苗、去根苗、去根去顶苗、顶芽四种外植体中,及在0.5%~0.9%琼脂含量和1.0~2.0 mmol?L-1 KH2PO4含量的培养条件下,采用07%琼脂含量和1.75 mmol?L-1KH2PO4含量培养黄瓜去根去顶苗的雌花诱导效果最好,其诱导率和雌花数分别达46%、54枚。
Abstract:
The synergistic effect of Spm and IAA,and the effects of agar concentration,KH2PO4 concentration on the induction of female flowers in steriled seedlings of Cucumis sativus for the establishment of an induction system of female flowers were reported. It was found that the induction rate and the amount of female flower in every trial were considerably low and even down to zero when Spm or IAA was respectively added to derooted seedlings cultured on MS basal medium,while the induction effect were obviously higher when 12 mg?L-1 Spm and 0.01 mg?L-1 IAA were added simultaneously,and no female flowers could be observed on the control medium,which showed that Spm and IAA had an significant synergistic effect on the induction of female flowers. Experiment was done with different explant of whole seedlings,derooted seedlings,detoped seedlings without root,top shoots or with agar concentration of 0.5%-0.9%,and with KH2PO4 concentration of 1.0-2.0 mmol?L-1,the highest induction effect for female flowers was achieved on medium of agar 0.7%,KH-2PO-4 1.75mmol?L-1 and from detoped seedlings without root,its induction rate and amount of female flower reched to 46%,54 respectively.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2010-04-29