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毛药忍冬花蕾抗氧化活性部位化学成分研究(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2015年04期
页码:
586-589
栏目:
植物化学与化学生物学
出版日期:
2015-07-20

文章信息/Info

Title:
Chemical constituents with antioxidative activity from the flower buds of Lonicera serreana
作者:
李树立1* 刘玉衡2
1. 河北经贸大学 生物科学与工程学院, 石家庄 050061; 2. 河北医科大学 基础医学院, 石家庄 050017
Author(s):
LI Shu-Li1* LIU Yu-Heng2
1. College of Biology Science and Engineering, Hebei Economy and Trade University, Shijiazhuang 050061, China; 2. Basic Medical College, Hebei Medical University, Shijiazhuang 050017, China
关键词:
毛药忍冬花蕾 DPPH自由基清除法 抗氧化活性 绿原酸 黄酮
Keywords:
flower buds of Lonicera serreana DPPH radical scavenging assay antioxidant activity chlorogenic acids flavonoids
分类号:
Q946
DOI:
-
文献标识码:
A
摘要:
毛药忍冬(Lonicera serreana)为忍冬属(Lonicera)植物,其花和果实入药,具有清热解毒、凉散风热之功效,但至今缺乏系统化学成分及药理活性研究。为了寻找毛药忍冬中天然抗氧化活性成分,进一步开发利用忍冬属药用植物资源,该研究以DPPH自由基清除法为活性指导,首次对毛药忍冬干燥花蕾75%乙醇提取物的不同极性萃取部位进行抗氧化活性测试,结果发现乙酸乙酯萃取物表现出最强的抗氧化活性(平均清除率为89.45%)。进一步应用现代色谱手段(硅胶柱色谱、Sephadex LH-20凝胶柱色谱等),从毛药忍冬花蕾的乙酸乙酯萃取物中分离单体化合物,运用现代光谱分析技术(MS、1H-NMR、13C-NMR、COSY、HSQC、HMBC、ROESY),并结合文献数据鉴定化合物的化学结构。结果表明:从毛药忍冬干燥花蕾75%乙醇提取物中共分离得到9个化合物,分别鉴定为4个酚酸类化合物:绿原酸(1)、绿原酸甲酯(2)、绿原酸乙酯(3)、咖啡酸(4); 4个黄酮类化合物:木犀草素(5)、木犀草素-7-O-β-D-葡萄糖苷(6)、槲皮素(7)、槲皮素-3-O-β-D-葡萄糖苷(8); 1个甾醇类化合物:β-谷甾醇(9)。所有化合物均为从毛药忍冬花蕾中首次分离得到。研究结果可为抗氧化类相关产品的开发提供科学依据。
Abstract:
Lonicera serreana is the endemic species in China from the genus Lonicera. The flowers and fruits of L. serreana were commonly used in the traditional Chinese medicine for the treatment of various diseases, but there was no study on the chemical constituents of L. serreana. To study the chemical constituents with antioxidative activity from the flower buds of L. serreana, DPPH radical scavenging assay was used for screening the active fractions of 75% ethanol extract. The highest antioxidant activity(89.45%)was observed in the ethyl acetate layer of the extract. Further chemical investigation led to the isolation of nine compounds by modern chromatographic method(chromatography on silica gel and Sephadex LH-20 columns). Their structures were elucidated by modern spectrum analysis(MS, 1H-NMR, 13C-NMR, COSY, HSQC, HMBC, ROESY). As a result, four chlorogenic acids, four flavonoids and one sterol compound were identified from the ethyl acetate layer of the extract. All of these compounds were isolated from the flower buds of L. serreana for the first time. The chemical constituent of the flower buds of L. serreana was deeply studied under the active guidance of DPPH radical scavenging assay, therefore this study would accumulate the fundamental data for antioxidant related products development.

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备注/Memo

备注/Memo:
收稿日期: 2014-10-02修回日期: 2015-03-25
基金项目: 河北省科技支撑计划项目(10237101D-9)
作者简介: 李树立(1963-),河北石家庄人,硕士,副教授,主要从事植物化学成分与抗氧化活性研究,(E-mail)lishuli6318@sina.com。
更新日期/Last Update: 2015-07-20