我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

苎麻叶绿体SSR引物筛选及分析(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2015年04期
页码:
464-467
栏目:
系统与进化
出版日期:
2015-07-20

文章信息/Info

Title:
Screening and analysis of chloroplast SSR primers in ramie
作者:
贺燕飞12 李同建2 吴永恒2 赵珊红2 徐玲玲2 廖 亮2*
1. 江西农业大学 园林与艺术学院, 南昌 330045; 2. 九江学院 药学与生命科学学院, 江西 九江 332000
Author(s):
HE Yan-Fei1 2 LI Tong-Jian2 WU Yong-Heng2 ZHAO Shan-Hong2 XU Ling-Ling2 LIAO Liang2*
1. College of Landscape and Art, Jiangxi Agricultural University, Nanchang 330045, China; 2. School of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 33200, China
关键词:
苎麻 叶绿体SSR 引物筛选
Keywords:
ramie cpSSR primers screen
分类号:
Q949
DOI:
-
文献标识码:
A
摘要:
叶绿体微卫星标记为单亲遗传(除部分裸子植物外),有独立的进化路线,它在植物遗传多样性、群体遗传结构、系统发育分析及杂种鉴定等研究上用途广泛,是研究谱系地理的有效手段。苎麻的主产地和主要分布区均在我国,但其谱系地理研究目前尚未见有报道。该研究选择来自全国不同地区的52个苎麻样本,利用聚丙烯酰胺凝胶电泳法,在23对已知通用叶绿体SSR引物中筛选出适用于苎麻谱系地理研究的SSR引物,并利用筛选到的多态引物对52个苎麻样本进行聚类分析和单倍型网络图分析。结果表明:从23对通用引物中共筛选出16对适用于苎麻的多态引物,其平均多态信息含量为0.105 3,虽然以上引物多态性较低,但能够用于野生苎麻的遗传分析研究; 这52个苎麻样本聚为10支,分为8个单倍型,初步分析表明叶绿体SSR遗传变异速率较慢,不适用于苎麻种内的系统发育研究,但以上引物能够检测苎麻种内单倍型变异,可用于苎麻的谱系地理研究。
Abstract:
Given a maternal inheritance nature(except for some gymnosperms)and a low frequency of genetic recombination, chloroplast satellites markers are especially useful in plant genetic diversity analysis, population genetic structure analysis, phylogenetic analysis and hybrid identification, which is an effective means of phylogeography. The main producing area and main distribution area of ramie is in China,but the phylogeography research on ramie have not been reported. This article’s aim is to screen polymorphism cpSSR primers appropriate for phylogeography from universal cpSSR primers. In this paper,23 pairs of universal cpSSR primers were screened using 52 ramie samples from different locations all over the country,by polyacrylamide gel electrophoresis. And clustering analysis and cpDNA haplotypes analysis of 52 ramie samples were conducted using the screened primers. Sixteen out of them were polymorphic, and the average polymorphism information content of them was 0.105 3. Although the polymorphism of these primers was low,they could be used in the research of genetic analysis in ramie. Fifty-two samples were clustered into ten groups and divided into eight haplotypes,and primarily analysis showed that the genetic variation of cpSSR remains slow, these primers were not suitable for phylogentic research. However, these primers were able to detect haplotype variation, and suitable for the phylogeography research on ramie.

参考文献/References

Birky CW,Maruyama T,Fuerst P. 1983. An approach to population and evolutionary genetic theory for genes in mitochondria and chloroplasts,and some results[J]. Genetics,103:513-527
Benbouza H,Jacquemin JM,Baudoin JP,et al. 2006. Optimization of a reliable,fast,cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels[J]. Biotechnol Agron Soc Environ,10:77
Biss P,Freeland J,Silvertown J,et al. 2003. Successful amplification of rice chloroplast microsatellites from century-old grass samples from the park grass experiment[J]. Plant Mol Bid Rep,21:249-257
Bostein D,White RL,Skolnick M,et al. 1980. Construction of a genetic linkage map in a using restriction fragment length polymorphisms[J]. Am J Genet,32(3):314-331
Chen JH(陈建华),Xu Y(许英),Wang XF(王晓飞),et al. 2011. Advances in research on germplasms resources in genus Boehmeria Jacq.(苎麻属植物资源基础研究进展)[J]. J Plant Gen Res(植物遗传资源学报),12(3):346-351
Chung SM,Staub JE. 2003. The development and evaluation of consensus chloroplast primer pairs that possess highly variable sequence regions in a diverse array of plant taxa[J]. Theor Appl Genet,107(4):757-767
Doyle JJ,Doyle JL. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J].Phytochem Bull,19(1):11-15
Kaundun SS,Matsumoto S. 2002. Heterologous nuclear and chloroplast microsatellite amplitication and variation in tea Camellia sinensis [J]. Genome, 45(6):1 041-1 048
Liao L(廖亮),Li TJ(李同建),Liu ZL(刘中来),et al. 2009. Phylogenetic relationship of Ramie and its wild relatives based on cytogenetics and DNA sequences(基于细胞学和DNA序列的苎麻与其野生近缘类群系统关系研究)[J]. Acta Agron Sin(作物学报),35(10):1 778-1 779
Lian C,Oishi R,Miyashita N,et al. 2003. Genetic structure and reproduction dynamics of Salix reinii during primary succession on Mount Fuji,as revealed by nuclear and chloroplast microsatellite analysis[J]. Mol Ecol,12:609-618
Petit RJ,Duminil J,Fineschi S,et al. 2005. Comparative organization of chloroplast,mitochondrial and nuclear diversity in plant populations[J]. Mol Ecol,14:689-701
Provan J,Powell W,Hollingsworth PM. 2001. Chloroplast microsatellites:new tools for studies in plant ecology and evolution[J].Trends Ecol Evol,16:142-147
Sukhotu T,Kamijima O,Hosaka K. 2004. Nuclear and chloroplast DNA differentiation in Andean potatoes[J].Genome,47:46-56
Tang DL(唐冬丽),Jia HH(贾红辉),Jiang JX(蒋建雄),et al. 2011. Study on phylogenetic relationships of Boehmeria based on TrnL-F regions(利用TmL-F序列探讨苎麻属植物的系统发育关系)[J]. Lett Biotechnol(生物技术通讯),22(1):45-48
Wei X(魏潇),Zhang QP(章秋平),Liu WS(刘威生),et al. 2013. Distribution analysis of chloroplast microsatellite in chloroplast DNA in peach(Prunus persica L.)(桃叶绿体全序列微卫星分布规律研究)[J]. Liaoning Agric Sci(辽宁农业科学),(2):5-8
Xie XB(谢晓兵),Yu JW(于霁雯),Wu M(吴嫚),et al. 2011. Analysis of SSCP for cotton differentially expressed genes related to fiber development(利用SSCP技术分析棉花纤维差异表达的基因)[J]. Cotton Sci(棉花学报),23(4):306-310

备注/Memo

备注/Memo:
收稿日期: 2014-11-21修回日期: 2015-02-29
基金项目: 国家自然科学基金(31060050); 江西省自然科学基金(2008GZN0044); 江西省教育厅重点科研项目(GJJ08431); 九江市科技支撑计划项目。
作者简介: 贺燕飞(1987-),女,河南三门峡人,硕士研究生,主要研究方向为植物系统进化,(E-mail)405870034@qq.com。*通讯作者: 廖亮,博士,教授,研究方向为植物分子生物学,(E-mail)Liaol58@163.com
更新日期/Last Update: 2015-07-20