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稀土元素镧和铈对冬凌草再生植株生长及次生代谢产物的影响(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2015年03期
页码:
437-441
栏目:
植物化学与化学生物学
出版日期:
2015-05-20

文章信息/Info

Title:
Effects of rare-earth elements La3+and Ce3+ on the growth of Rabdosia rubescens regeneration plant and the accumulation of main secondary metabolites
文章编号:
1000-3142(2015)03-0437-05
作者:
董诚明1* 曹利华1 苏秀红1 张艳贞2 乔毅琳1 姚 锋1
1. 河南中医学院, 郑州 450008; 2. 新乡医学院 三全学院, 河南 新乡 450003
Author(s):
DONG Cheng-Ming1* CAO Li-Hua1 SU Xiu-Hong1 ZHANG Yan-Zhen2 QIAO Yi-Lin1 YAO Feng1
1. Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China; 2. Sanquan Medical College of Xinxiang, Xinxiang 450003, China
关键词:
冬凌草 稀土元素 甲素 乙素 迷迭香酸
Keywords:
Rabdosia rubescens rare earth elements oridonin ponicidin rosmarinic acid
分类号:
Q943.1; R282,284.1
DOI:
10.11931/guihaia.gxzw201312042
文献标识码:
A
摘要:
以冬凌草无菌苗为材料,在冬凌草再生植株时期,添加不同浓度的稀土元素镧和铈,用重量法测定其单株平均鲜重及干重,用高效液相法测定冬凌草甲素、乙素、迷迭香酸的含量,参考农作物、园艺作物种质评比的方法,根据冬凌草生产中各项指标的重要性,设置权重,计算加权后得到综合评分对其进行综合评价,探讨稀土元素镧、铈对冬凌草再生植株产量及次生代谢产物冬凌草甲素、乙素、迷迭香酸含量的影响。结果表明:空白对照组综合评分为62.99分,添加稀土元素铈的条件下,1 μmol·L-1 CeCl3·7H2O评分最高为87.96分,添加稀土元素镧的条件下,5 μmol·L-1 LaCl3·6H2O评分最高为74.44分,这表明CeCl3·7H2O对冬凌草再生植株生长及次生代谢产物积累的促进作用优于LaCl3·6H2O; 适宜浓度的镧、铈能促进冬凌草再生植株的生长及次生代谢产物冬凌草甲素、冬凌草乙素、迷迭香酸的合成,而高浓度10 μmol·L-1 LaCl3·6H2O具有抑制的情况。该研究结果将对进一步研究稀土元素对冬凌草再生植株生长的促进作用机制奠定基础,为植物组织培养中如何科学合理地使用稀土元素提供理论依据。
Abstract:
Aseptic seedlings of Rabdosia rubescens were used as the experiment material. In R. rubescens regeneration period, the rare-earth elements La3+or Ce3+ with different concentrations were added in the culture medium. Fresh and dry weight of the plant were determined by gravimetric method. HPLC method was used for the determination of oridonin, ponicidin and rosmarinic acid content. We determined weight according to evaluating methods of agricultural and horticultural crops and the importance of indexes in the production of R. rubescens. The quality synthetic evaluation was also carried out according to weighted mark. The effects of rare earth elements La3+ and Ce3+ on R. rubescens tissue culture and the contents of oridonin, ponicidin, rosmarinic acid in R. rubescens were studied. The synthetic score of blank control group was 61.51 points,under the condition of 1 μmol·L-1 CeCl3·7H2O, the score reached up to the highest value of 87.20 points. But under the condition of 5 μmol·L-1 LaCl3·6H2O,the score 74.44 points. The results of this experiment showed that CeCl3·7H2O had more positive promoting effects on the growth of regeneration plant of R. rubescens and the accumulation of main secondary metabolites than LaCl3·6H2O. The optimal concentration of Ce3+or La3+ could promote the growth of regeneration plant of R. rubescens and secondary metabolites oridonin,ponicidin and rosmarinic synthesis. However,La3+ concentration in high level inhibited the growth of R. rubescens plant regeneration. The experiment would lay a foundation for further study on the promotion effect on the growth of regeneration plant of R. rubescens. The study of R. rubescens could provide theoretical basis for the rational utilization of rare earth elements.

参考文献/References

Chinese pharmacopoeia commission(国家药典委员会). 2010. Pharmacopoeia of the People’s Republic of China(中华人民共和国药典)[M]. Beijing(北京):China Medical Science Press(中国医学科技出版社):106
Chen WJ(陈为钧),Gu YH(顾月华),Wang SB(王圣兵),et al. 2000. Effect of Lanthanum on RuBPcase activity of tobacco seedlings(镧对烟草RuBPcase活性影响的研究)[J]. J Rare Earth(中国稀土学报),18(3):258-261
Chen WJ(陈为钧),Tao Y(陶冶),Hu TD(胡天斗),et al. 2001. Effects of Ln3+ on inhibition of tobacco RuBPcase(稀土离子对烟草RuBPcase的抑制作用)[J]. J Rare Earth(中国稀土学报),19(4):362-365
Dong CM(董诚明),Xu P(徐鹏),Su XH(苏秀红),et al. 2011. Effects of trace elements Znic on yield and effective components of Rabdosia rubescens(锌对冬凌草产量及其有效成分含量的影响)[J]. Chin J Exp Trad Med Form (中国实验方剂学杂志),17(3):58-60
He YJ(何跃君),Xue L(薛立). 2005. Biological effects of rare earth elements and their action mechanisms(稀土元素对植物的生物效应及其作用机理)[J]. Chin J Appl Ecol (应用生态学报),16(10):1 983-1 989
Liu CJ(刘晨江),Zhao ZH(赵志鸿). 1998. Development of Rabdosia Rubescens [Hemsl. ]Hara(冬凌草的研究进展)[J]. Chin Pharm J(中国药学杂志),33(10):577-581
Liu C(刘晨),Li DJ(李冬杰),Li N(李楠),et al. 2009. Effects of rare earth elements on the synthesis of rubescensin A in Robdosia rubescens cell(稀土元素对冬凌草细胞合成冬凌草甲素的影响)[J]. J Anhui Agric Sci(安徽农业科学),37(13):5 965-5 966,5 969
Li JC(李继成),Liu CJ(刘晨江),Sun HD(孙汉董). 1986. Research on the structure of lushan rubesccnsin B and C(鲁山冬凌草乙素和丙素的结构研究)[J]. Acta Bot Yunnan(云南植物研究),8(1):93-97
Li W(李伟),Liang JS(梁建生),Li YR(李幼荣),et al. 2002. Studies on biological dosage effect of rare earth element on protein(稀土元素对生物体蛋白的剂量效应研究进)[J]. Biotechnology(生物技术),12(1):42-44
Tian M(田蜜). 2009. The germplasm resource evaluation and preliminary screening of excellent strains about Curcuma longa,which is produced in Sichuan Province(川产姜黄的种质资源评价及优良株系初步筛选研究)[D]. Chengdu(成都):Chengdu University of Tradition Chinese Medicine(成都中医药大学)
Xu ZC(徐忠传),Yu D(郁达),Zhou JY(周静亚). et al. 2006. The effects of Eu and Y on regeneration and growth of test-tube plantlet for Oxalis violacea(稀土元素铕和钇对紫叶酢浆草试管苗生长的影响)[J]. Biol Bull(生物科技通报),(s1):330-333
Xie HG(解惠光). 1991. A study on the application of rare earth elements on agriculture(中国稀土元素在农业上应用研究进展)[J]. Chin Sci Bull(科学通报),36(8):561-564
Xue JP(薛建平),Xu M(徐敏),Zhang AM(张爱民). 2004. Effects of rare-earth elements Cerium on “Red Globe”grape plantlets in vitro(稀土元素铈对“红地球”葡萄组培苗生长的影响)[J]. Acta Hortic Sin(园艺学报),31(3):369-371
Yuan XF(袁晓凡),Zhao B(赵兵),Wang YC(王玉春). 2005. Application of rare earth elements in medicinal plant cell and tissue culture(稀土元素在药用植物细胞和组织培养中的应用)[J]. Chin Bull Bot(植物学通报),22(1):115-120
Zhou J(周洁),Zhang J(张霁),Gou LP(郭兰萍),et al. 2010. Effects of lanthanum on leaf photosynthesis and artemisinin accumulation in Artemisia annua(稀土元素镧对黄花蒿光合作用及青蒿素积累的影响)[J]. Chin Trad Herb Drug(中草药),41(8):1 371-1 374
Zhao YQ(赵英琪),Wang NJ(王乃江),Zhao ZY(赵智渊). 1999. A research of effects of Ce rare earth on photosynthesis and drought resistance of Prunus armeniaca�/i>215;sibirica(稀土铈对大扁杏光合作用和抗旱性影响的研究)[J]. Acta Bot Boreal-Occident Sin(西北植物学报),19(5):54-58
Wang YX(王永祥),Liu B(刘斌). 2006. Development of fluorescent indicators of intracellular free Ca2+(细胞内游离 Ca2+的荧光指示剂研究进展)[J]. J Xi’an Univ Arts & Sci:Nat Sci Ed(西安文理学院学报·自然科学版),9(1):21-24
Xu GH(徐国华),Zhang SL(张绍玲),Zhang CY(张超英),et al. 2003. Changes in free Ca2+ distribution in pollen cell after self-and cross pollination in Pyrus serotina Re hd(梨自花与异花授粉后花粉胞内游离 Ca2+分布的变化)[J]. J Plant Physiol & Mol Biol(植物生理与分子生物学学报),29(2):97-103
Yu XC(余晓丛),Zhang SY(张少英),Na R(娜仁). 2013. Optimization of fluorescence detection conditions of calcium ion in the cell in sugar beet(荧光指示剂检测甜菜胞内钙离子的条件优化)[J]. J Northeast Agric Univ(东北农业大学学报),44(1):61-64
Yuan JH(袁建辉),Yang H(杨慧),Tang H(唐焕),et al. 2011. A cell-based detection of ciguatoxin usingsodium fluorescence probe(钠离子荧光探针用于检测雪卡毒素的细胞毒性)[J]. J Southern Med Univ(南方医科大学学报),31(4):653-655
Zhang JT,Liu H,Sun J, et al. 2012. Arabidopsis fatty acid desaturase FAD2 is required for salt tolerance during seed germination and early seedling growth[J]. PLoS One,7(1):1-12
Zhang WH,Rengel Z,Kuo J. 1998. Determination of intracellular Ca2+ in cells of intact wheat roots:loading of acetoxymethyl ester of Fluo-3 under low temperature[J]. Plant J,15(1):147-151
Zhou P(周平),Sun TE(孙天恩),Ye MW(叶梦炜), et al. 1995. Study on the loading of Ca2+ fluorescent indicator into protoplasts(钙离子荧光指示剂导入植物细胞原生质体的研究)[J]. Wuhan Univ J:Nat Sci Ed(武汉大学学报·自然科学版),41(2):208-212

备注/Memo

备注/Memo:
收稿日期: 2014-05-02修回日期: 2014-10-1
基金项目: 国家自然科学基金(81173486); 国家科技重大专项(2012ZX09304006)。
作者简介: 董诚明(1963-),男,内蒙古四子王旗人,教授,主要从事中药资源学的研究与开发,(E-mail)dcm663@sina.com。
更新日期/Last Update: 2015-05-20