我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

过路黄自然三倍体的发现(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2015年03期
页码:
373-377
栏目:
遗传与育种
出版日期:
2015-05-20

文章信息/Info

Title:
Discovery of natural triploid in Lysimachia christinae
文章编号:
1000-3142(2015)03-0373-04
作者:
刘晓明1 陈龙清 2 郑 伟1*
1. 昆明理工大学 现代农业工程学院, 昆明 650224; 2. 华中农业大学 园艺林学学院, 武汉 430070
Author(s):
LIU Xiao-Ming1 CHEN Long-Qing2 ZHENG Wei1*
1. College of Modern Agricultural Engineering, Kunming University of Science and Technology, Kunming 650224, China; 2. College of Horticulture and Forestry, Huazhong Agriculture University, Wuhan 430070, China
关键词:
过路黄 形态 核型 同源三倍体
Keywords:
Lysimachia christinae morphology karyotype autotriploid
分类号:
Q943; Q949.773.2
DOI:
10.11931/guihaia.gxzw201404001
文献标识码:
A
摘要:
过路黄为报春花科的多年生匍匐草本植物,是有名的中药材,也是十分优质的园林地被植物。该文报道了过路黄的自然三倍体,以期为过路黄的开发利用提供新的参考资料。该研究先将湖北省武汉狮子山和黄石月亮山的过路黄居群引种至同一实验地进行栽培,然后观测其植株形态和叶片气孔大小,并利用常规压片法进行细胞染色体的计数与核型分析。结果表明:源自黄石月亮山的过路黄的叶片长度、叶片宽度、叶片面积、花瓣长度、花瓣宽度、花瓣面积及叶背气孔面积均显著高于源自武汉狮子山的过路黄; 黄石月亮山的过路黄核型公式为3n=36=3m+3sm+6st+24t,与武汉狮子山的过路黄核型2n=24=2m+2sm+4st+16t不同。基于上述形态与核型数据,该文认为黄石月亮山的过路黄居群为自然三倍体。过路黄自然三倍体的发现丰富了过路黄的多倍体资料,不仅能为过路黄的遗传多样性及进化研究提供参考,还能为过路黄的药用、园林应用及其新品种的培育提供优良材料。
Abstract:
Lysimachia christinae Hance(Christina loosestrife),a mat-forming perennial herb from the Primulaceae family, is a famous Chinese medicinal material and excellent ground cover plant for landscaping. The natural triploid of L. christinae is reported here so as to provide new reference for the development and utilization of L. christinae. In this study, L. christinae plants introduced from Lion Mountain population in Wuhan and Moon Mountain population in Huangshi were cultivated in the same text site. And then the plant morphology and the stoma size were observed. The chromosome numbers and karyotypes were investigated by the conventional pressing plate method. The results showed that the leaf length, leaf width, leaf area, length of petal, petal width, petal area and leaf stoma area of L. christinae from Moon Mountain population in Huangshi were significantly higher than those of L. christinae from Lion Mountain population in Wuhan. In addition,the karyotype formula of L. christinae from Moon Mountain population in Huangshi was 3n=36=3m+3sm+6st+24t,which was different with that of L. christinae from Lion Mountain population in Wuhan(2n=24=2m+2sm+4st+16t). Therefore, in this paper, the L. christinae from Moon Mountain population in Huangshi was identified as natural triploid based on abovementioned morphology and chromosome datum. The discovery of natural triploid in L. christinae enriched the polyploidy data of L. christinae. It would not only provide reference for research on genetic diversity and evolution of L. christinae,but also supply the excellent material for medicine, landscaping and breeding.

参考文献/References

Arano H. 1963. Cytological studies in subfamily Carduoideae(Compositae)of Japan. IX. The karyotype analysis and phylogenic considerations on Pertya and Ainsliaea[J]. Bot Mag Tokyo,76:32-39
Chang HT(常海涛),Kong WL(孔维梁),Tu PF(屠鹏飞). 2004. Chemical and pharmacological advances of study on Lysimachia(珍珠菜属植物化学成分及药理作用研究进展)[J]. Chin J Chin Mat Med(中国中药杂志),29(4):295-298
Chen FH(陈封怀),Hu QM(胡启明). 1989. Flora of China(中国植物志)[M]. Beijing(北京):Science Press(科学出版社):1-160
Karlsdottir L,Hallsdottir M,Thorsson AT,et al. 2008. Characteristics of pollen from natural triploid Betula hybrids[J]. Grana,47(1):52-59
Levan A,Fredga K,Sandberg AA. 1964. Nomenclature for centromeric position on chromosomes[J]. Hereditas,54(2):201-220
Li MX(李懋学),Chen RY(陈瑞阳). 1985. A suggestion on the standardization of karyotype analysis in plants(关于植物核型分析的标准化问题)[J]. J Wuhan Bot Res(武汉植物学研究),4(2):297-302
Liu XS(刘学生),Chen L(陈龙),Wang JX(王金鑫), et al. 2013. Discovery and identification of natural triploid ploidy of Chinese jujube cultivar ‘Pingguozao’(‘苹果枣’自然三倍体倍性的发现与鉴定)[J]. Acta Hortic Sin(园艺学报),40(3):426-432
Liu KH(刘可慧),Yu FM(于方明),Zhang QM(张秋明),et al. 2010. Regenerating triploid citrus plants by embryo rescue technique(应用胚挽救技术获得三倍体柑橘植株)[J]. Guihaia(广西植物),30(2):246-249
Shao JW(邵剑文),Li XH(李晓红),Han L(韩露),et al. 2004. A study on karyotypes of five species in Lysimachia(5种珍珠菜属植物的核型分析)[J]. Acta Bot Yunnan(云南植物研究),26(4):427-433
Stebbins GL. 1971. Chromosomal Evolution in Higher Plants[M]. London:Edward Arnold(Publishers)Ltd Sun AQ(孙爱群),Xiang H(向红),Tian YZ(田应洲),et al. 2007. Study on karyotypes of four species in Lysimachia (珍珠菜属四种植物的核型分析)[J]. J Wuhan Bot Res(武汉植物学研究),25(5):509-512
Yang SL,Lihua TLH,Tian JK,et al. 2009. Anti-tumor constituents of four medicinal plants from Lysimachia genus[J]. Planta Medica,75(4):412-412
Zheng W,Xu XD. 2011. Single-node stolonizing,an innovative propagation method for two landscape stoloniferous Lysimachia L. ground covers[J]. Inter Conf Agric & Biosyst Eng:317-319
Zheng W,Xu XD,Zhao KG,et al. 2009a. Lysimachia christinae ‘Zixin’:a new groundcover plant[J]. Hortic Sci,44(2):474-475
Zheng W,Xu XD,Dai H,et al. 2009b. Direct regeneration of plants derived from in vitro cultured shoot tips and leaves of three Lysimachia species[J]. Sci Hortic,122(1):138-141
Zhou XL(周筱玲),Liao L(廖亮),Chen Y(陈晔),et al. 1999. Study on karyotypes of two species in Lysimachia(Primulaceae)from China(两种过路黄的核型研究)[J]. Guihaia(广西植物),19(3):236-238
The review of higher plants glutamine synthetase(高等植物谷氨酰胺合成酶研究进展)[J]. J Biol (生物学杂志), 18(4): 1-3
Martin A,Lee J,Kichey T,et al. 2006. Two cytosolic glutamine synthetase isoforms of maize are specifically involved in the control of grain production[J]. Plant Cell,18:3 252-3 274
Masakazu T,Ulrika V,Brad B,et al. 2004. Arabidopsis thaliana GLN2-encoded glutamine synthetase is dual targeted to leaf mitochondria and chloroplasts[J]. Plant Cell,16:2 048-2 058
Miflin BJ,Habash DZ. 2002. The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops[J]. J Exp Bot,53:979-987
Morey KJ,Ortega JL,Sengupta-Gopalan C. 2002. Cytosolic glutamine synthetase in soybean is encloded by a multigene family,and members are regulated in an organ-specific and developmental manner[J]. Plant Physiol,128:182-193
Takashi T,Takeshi Y,Katsushige M,et al. 1998. γ-Glutamyl Transfer Reactions by glutaminase from Pseudomonas nitroreducens IFO 12694 and their application for the syntheses of theanine and γ-Glutamylmethylamide[J]. Biosci Biotechnol & Biochem,62(7):1 279-1 283
Sasaoka K,Kito M,Onishi Y. 1965. Some properties of the theanine synthesizing enzyme in tea seedlings[J]. Agric & Biol Chem,29:984-98
Shi CY(史成颖),Wan XC(宛晓春),Jiang CJ(江昌俊)et al. 2007. Method for high-quality total RNA isolation from tea plant[Camellia sinensis(L.)O. Kuntze](提取高质量茶树总RNA的方法研究)[J]. J Anhui Agric Univ(安徽农业大学学报), 34(3):360-363
Tsushida T,Takeo T. 1984. Occurrence of theanine in Camellia japonica and Camellia sasanqua seedlings[J]. Agric Biol Chem,48(11):2 861-2 862
Qiao FL(乔富廉). 2002. Analysis Determination Technique of Plant Physiology Experiment(植物生理学实验分析测定技术)[M]. Beijing(北京): China Agriculture Science and Technique Press(中国农业科技出版社):130
Shi CY(史成颖), Wan XC(宛晓春), Jiang CJ(江昌俊),et al. [J]. 2007. Method for high-guality total RNA isolation from tea plant(提取高质量茶树总RNA的方法研究)[J]. J Anhui Agric Univ (安徽农业大学学报), 34(3): 360-363
Wan XC(宛晓春). 2003. Tea Biochemistry(茶叶生物化学)[M]. 3rd Ed(第3版). Beijing(北京): China Agriculture Science and Technique Press(中国农业科技出版社): 32-34, 319-350
Yang XQ(杨贤强). 1987. Physiological and biochemical basis of nitrogen metabolism characteristics of tea plant[Camellia sinensis(L.)O. Kuntze](茶树氮代谢特点的生理生化基础)[J]. J Tea Fujian(福建茶叶),1:11-14
Yang XQ(杨贤强).1987. The physiological and biochemical basis of nitrogen metabolism characteristics in tea plant(茶树氮代谢特点的生理生化基础)[J]. Tea Fujian (福建茶叶), 1: 11-14 Zhang CF,Peng SB,Peng XX,et al. 1997. Response of glutamine synthetase isoforms to nitrogen sources in rice(Oryza sativa L.)roots[J]. Plant Sci,125:163-170
Zhu WX(朱文娴), Li XH(黎星辉), Wang LY(王丽鸳), et al. 2008. Construction of E.coli recombinant engineered strain for theanine biosynthesis with GS gene embedded(利用GS基因构建茶氨酸生物合成工程茵的研究). [J].J Tea Sci(茶叶科学), 28(4): 242-248

备注/Memo

备注/Memo:
收稿日期: 2014-06-24修回日期: 2014-08-21
基金项目: 国家自然科学基金(311015665,31360488)
作者简介: 刘晓明(1989-),男,湖南娄底人,硕士研究生,从事细胞生物学研究,(E-mail)1015883384@qq.com。 *通讯作者: 郑伟,副教授,硕士生导师,主要从事园林植物与应用研究,(E-mail)57280234@qq.com。
更新日期/Last Update: 2015-05-20