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二花蝴蝶草的组织培养及植株再生(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2015年02期
页码:
250-254
栏目:
遗传与育种
出版日期:
2015-04-20

文章信息/Info

Title:
Tissue culture and plant regeneration from Torenia biniflora
文章编号:
1000-3142(2015)02-0250-05
作者:
王瑛华1 石秋英1 陈雄伟1 陈 刚1* 金 红2
1. 肇庆学院 生命科学学院, 广东 肇庆 526061; 2. 深圳市仙湖植物园 科技部, 广东 深圳 518004
Author(s):
WANG Ying-Hua1 SHI Qiu-Ying1 CHEN Xiong-Wei1 CHEN Gang1* JIN Hong2
1. College of Life Sciences, Zhaoqing University, Zhaoqing 526061, China; 2. Department of Science and Technology, Shenzhen Fairylake Botanical Garden, Shenzhen 518004, China
关键词:
二花蝴蝶草 不定芽 不定根 再生植株
Keywords:
Torenia biniflora adventitious buds adventitious root regenerated plant
分类号:
Q943.1
DOI:
10.11931/guihaia.gxzw201307019
文献标识码:
A
摘要:
二花蝴蝶草(Torenia biniflora)为玄参科蝴蝶草属一年生植物,分布于广东、广西等亚热带地区,是一种观赏性较高的野生花卉。在自然生长条件下,二花蝴蝶草繁殖速度慢、增殖率低,而且花色和花型种类偏少,无法满足市场多样化的要求。植物组织培养技术为观赏植物的品种改良和新品种选育提供了新途径,目前蓝猪耳、蔓性蝴蝶草和单色蝴蝶草等蝴蝶草属植物的组织培养已获得成功,但二花蝴蝶草的组织培养尚未见有相关报道。该研究以二花蝴蝶草全展叶片为外植体,研究了培养基中添加不同种类和浓度植物生长物质对不定芽诱导和生长的影响,以及离子强度和不同浓度IBA对生根的影响。根据不定芽的诱导率和平均芽数筛选出最佳不定芽诱导培养基,并从生根率、平均根数和平均根长等方面筛选出最佳生根培养基。结果表明:不定芽诱导与植物生长物质的浓度和种类有关,以MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1培养基的诱导效果最佳; 二花蝴蝶草生根的最佳基本培养基为1/2MS,不同浓度的IBA对二花蝴蝶草的生根影响也不相同,其中以IBA(0.05 mg·L-1)诱导不定芽的生根效果最佳。该研究建立了二花蝴蝶草的高频离体再生体系,为二花蝴蝶草的快速繁殖和遗传转化研究奠定了基础。
Abstract:
Torenia biniflora is a member of Scrophulariaceae Torenia,and it is an annual flowering plant that widely grows in Chinese subtropical area,such as Guangdong Province and Guangxi Zhuang Autonomous Region. T. biniflora is a wild flower with high great ornamental value. However,in natural growth condition,the reproduction speed of T. Biniflora is slow,proliferation rate is low,and the kinds of flower colour and type are few,therefore,it is unable to meet the diversified need of market. Plant tissue culture technology provides a new way to improve and breed new varieties for ornamental plants. Up to now,a lot of members of Torenia plants have been successfully established in vitro regeration system through tissue culture,such as T. fournieri Linden,T. baillonii Susie Wong and T. concolor Lindl.,etc. While,tissue culture for T. Biniflora has never been reported. The main objective of this research was to establish in vitro regeneration system of T. Biniflora through tissue culture and study the effects of plant growth substances such as IBA,6-BA and NAA for clonal multiplication. In this study,we created a reliable protocol for regenerating T. biniflora plants in tissue culture by using leaf explants. The effects of different concentrations and combinations of plant growth substances on adventitious bud induction and growth were studied,and the effects of different ionic strengths of media and concentrations of IBA on rooting were analyzed. According to a series of indices,such as average number of buds,frequency of bud induction,frequency of rooting,average number of root and average length of root,the optimal media for inducing adventitious buds and rooting were screened respectively. The results showed that adventitious bud induction was related with the concentrations and combinations of plant growth regulator,and the optimal medium for buds induction was MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1. The best medium for rooting was 1/2MS as basal medium with IBA 0.05 mg·L-1. In this study the in vitro plant reproductive system of T. biniflora with high frequence was completely established,and it would provide an important experiment foundation for the research of rapid propagation and genetic transformation for T. biniflora.

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备注/Memo

备注/Memo:
收稿日期: 2014-05-05修回日期: 2014-09-17
基金项目: 肇庆市科技项目(2008N001); 肇庆学院自然科学基金(0731)。
作者简介: 王瑛华(1979-),女,陕西商洛人,博士,讲师,主要研究方向为植物细胞生理与逆境信号转导,(E-mail)wangyinghua_118@163.com。 *通讯作者: 陈刚,博士,副教授,主要从事植物细胞工程和基因工程方面的教学与研究工作,(E-mail)chengang@zqu.edu.cn。
更新日期/Last Update: 2015-04-20