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缺锌和HCO3-处理对诸葛菜和油菜有机酸特征的影响(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2015年02期
页码:
206-212
栏目:
生理与发育
出版日期:
2015-04-20

文章信息/Info

Title:
Effects of zinc deficiency and bicarbonate treatments on the characteristics of organic acids of Orychophragmus violaceus and Brassica napus
文章编号:
1000-3142(2015)02-0206-07
作者:
赵 宽1 2 吴沿友23* 周葆华1
1. 安庆师范学院 资源环境学院, 安徽 安庆 246011; 2. 现代农业装备与技术教育部重点实验室, 江苏大学农业工程 研究院, 江苏 镇江 212013; 3. 中国科学院地球化学研究所, 环境地球化学国家重点实验室, 贵阳 550002
Author(s):
ZHAO Kuan1 2 WU Yan-You2 3* ZHOU Bao-Hua1
1. School of Environmental Resources, Anqing Normal University, Anqing 246011, China; 2. Key Laboratory of Modern Agricultural Equipment and Technology of Ministry of Education, Institute of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China; 3. State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China
关键词:
有机酸 植物器官 根系分泌物 喀斯特适生性 诸葛菜
Keywords:
organic acid plant organs root exudates the adaptability to karst Orychophragmus violaceus
分类号:
Q945
DOI:
10.11931/guihaia.gxzw201410015
文献标识码:
A
摘要:
以诸葛菜和油菜为材料,水培环境下设置4个不同的缺锌和碳酸氢根离子胁迫处理,分别为+Zn0(含Zn且不加HCO3-的处理组),+Zn10(含Zn且加10 mmol·L-1 HCO3-的处理组),-Zn0(缺Zn且不加HCO3-的处理组)和-Zn10(缺Zn且加10 mmol·L-1 HCO3-的处理组),利用离子色谱法分析了4个处理的两种植物幼苗器官(根、茎、叶)及根系分泌物中的有机酸特征。结果表明:(1)高浓度碳酸氢根离子处理显著增加了两种植物器官及根系分泌的有机酸总量,尤其是在缺锌和高浓度碳酸氢根离子双重胁迫下(-Zn10处理),诸葛菜器官和根系分泌的有机酸比油菜更敏感,草酸、柠檬酸和苹果酸是诸葛菜器官和根系分泌物中的优势酸,这三种有机酸的含量分别占其有机酸总量的75%及以上;(2)叶片是两种植物有机酸产生的主要器官,有机酸的含量和分配比例从地上部分(叶和茎)到地下部分(根)减少;(3)两种植物器官和根系分泌物中的有机酸变化趋势一致,叶片中有机酸主要来源于暗呼吸过程和光呼吸过程,其他器官和根系分泌物中的有机酸主要来源于暗呼吸过程;(4)诸葛菜对缺锌和高浓度碳酸氢根离子的适应能力强于油菜,为诸葛菜的喀斯特适生性和低锌和高浓度碳酸氢根离子环境(如喀斯特环境)的生态修复提供了理论依据。
Abstract:
The characteristics of organic acids in plant organs(roots,stems and leaves),and root exudates of seedlings of Orychophragmus violaceus and Brassica napus were investigated under the stress of Zn deficiency and excessive bicarbonate by ion chromatography,the two plant species were hydroponically grown and cultured in four different treatments: +Zn0(the treatment of adequate Zn and none HCO3-),+Zn10(the treatment of adequate Zn and HCO3- addition),-Zn0(the treatment of Zn deficiency and none HCO3-),and -Zn10(the treatment of Zn deficiency and HCO3- addition),respectively. The results were as follows:(1)The total content of organic acids in plant organs and root exudations of the two plant species were significantly increased under excessive bicarbonate treatment,particularly under the dual treatment of Zn deficiency and excessive bicarbonate conditions(-Zn10 treatment),the organic acids in organs and root exudates of O. violaceus were more sensitive than that of B. napus,oxalic,citric and malic acids were the dominant organic acids in organs and root exudates of O. violaceus,the content of these three organic acids took account for more than seventy five percent of the total content of organic acids in organs and root exudates of O. violaceus;(2)The leave was the main region of organic acids production of the two plant species,the content and the allocated proportion of organic acids decreased from aboveground parts(leaves and stems)to belowground parts;(3)The variation trends of organic acids contents of organs were identical with that of root exudates in O. violaceus and B. napus,and the source of organic acids in leaves of the two plant species was derived from the processes of dark respiration and photorespiration,while the source of organic acids in other organs such as stems and roots,as well as the source of organic acids in root exudates were come from the process of dark respiration;(4)The adaptability to the environment of low Zn and excessive HCO3- of O. violaceus was higher than that of B. napus,which provided evidences for the adaptability to karst environment of O. violaceus,and ecological restoration in environments with low Zn and excessive HCO3-,such as a karst area.

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备注/Memo

备注/Memo:
收稿日期: 2014-10-11修回日期: 2014-12-29
基金项目: 国家重点基础研究发展计划项目(2013CB956703); 中国科学院战略性先导科技专项重大课题(XDA05070400); 江苏省高校优势学科项目(苏财教[2011]8号); 安庆师范学院人才引进项目(140001000032)。
作者简介: 赵宽(1986-),男,安徽池州人,博士,讲师,主要从事植物逆境生理生化的研究,(E-mail)henry3408@126.com。 *通讯作者: 吴沿友,博士,研究员,从事生态环境和地球化学方面的研究,(E-mail)yanyouwu@ ujs.edu.cn
更新日期/Last Update: 2015-04-20