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喀斯特石生穗枝赤齿藓抗氧化防御系统对干旱胁迫的响应(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2015年02期
页码:
200-205
栏目:
生理与发育
出版日期:
2015-04-20

文章信息/Info

Title:
Responses of antioxidant defense system of Erythrodoutium juluceum to drought stress in rocky desertification of karst areas
文章编号:
1000-3142(2015)02-0200-06
作者:
张显强12 李 超3 王世杰1* 孙 敏4
1. 中国科学院地球化学研究所 环境地球化学国家重点实验室, 贵阳 550002; 2. 贵州警官职业学院, 贵阳 550005; 3. 贵州省农业 科学研究院, 贵阳 550006; 4. 西南大学 生命科学学院/三峡库区生态环境与生物资源省部共建国家重点实验室, 重庆 400715
Author(s):
ZHANG Xian-Qiang12 LI Chao3 WANG Shi-Jie1* SUM Min4
1. State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China; 2. Guizhou Police Officer Vocational College, Guiyang 550005, China; 3. Guizhou Province Academy of Agriculture Sciences, Guiyang 550006, China; 4. School of Life Sciences, Southwest University/State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, Chongqing 400715, China
关键词:
喀斯特石漠化 穗枝赤齿藓 抗氧化酶系统 干旱胁迫
Keywords:
rocky desertification of karst E. juluceum antioxidase system drought stress
分类号:
Q945.79
DOI:
10.11931/guihaia.gxzw201
文献标识码:
A
摘要:
为科学选择石漠化环境恢复治理的植物材料和深入了解岩生植物的耐旱机制,以喀斯特石生穗枝赤齿藓(Erythrodoutium juluceum)为实验材料,研究了干旱胁迫对其抗氧化酶防御系统的影响。结果表明:超氧化酶歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性,超氧阴离子(O-2·)、丙二醛(MDA)含量呈现出先升后降的趋势; 类胡萝卜素(Car)含量一直升高,质膜相对透性呈现出“抛物线”的变化趋势,可溶性蛋白含量波动变化。因此,干旱胁迫早期由于O-2·等活性氧的增加而启动活性氧清除系统进行清除,是抵御干旱的一种协同反应; 后期穗枝赤齿藓依然保持较强的自由基清除能力,具有极强的耐旱能力。所以在长期的进化过程中,喀斯特石生藓类形成具有适合干旱变化的系列生理和代谢机制,通过形态上的变化来降低水分的散失,通过生理上的调整来应付环境的恶劣变化,这对于在石漠化地区揭示苔藓植物的抗逆机制,利用苔藓植物的先锋拓荒作用治理石漠化生态环境以及对退化生态系统进行人工恢复治理具有重要价值。
Abstract:
The research focuses on the antioxidant defense system of E. juluceum by PEG-6000 simulated drought stress conditions. The contents of E. juluceum superoxide dismutase dismutase(SOD), catalase(CAT), peroxidase(POD)activity, superoxide anion(O-2·)and malondialdehyde(MDA)content increased, and at late declined. The carotenoids(Car)content always increasing. As for the relative permeability of membrane, E. juluceum were shown a trend of the parabola and increased in varying degrees, and the soluble protein content in variation of fluctuate. Therefore, the increases in drought stress early O-2· and other reactive oxygen species(ROS)induce enzymatic and non-enzymatic scavenging system to start to clear ROS. Keep the radical scavenging ability and strong ability of drought tolerance. So, in the long process of evolution, karst epilithic mosses formed with a series of physiological changes and metabolic mechanism for drought, through the change in morphology to reduce water loss, bad by changes in physiological regulation to cope with the environment, which for the mechanism of resistance to reveal the bryophytes inrocky desertification area, the use of pioneer the pioneering role of bryophytes in rocky desertification control and ecological environment have important value of artificialrestoration of degraded ecosystem.

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备注/Memo

备注/Memo:
收稿日期: 2014-08-18修回日期: 2014-10-20
基金项目: 国家自然科学基金(41463006); 国家科技支撑计划“973”项目(2013CB956700); 国家重点实验室开放基金(SKL-2010-03)。
作者简介: 张显强(1976-),男,博士,副教授,博士后,研究方向为喀斯特植物生理生态,(E-mail)zhangxianqiang@126.com。 *通讯作者: 王世杰,研究员,博士生导师,(E-mail)wangshijie@vip.skleg.cn。
更新日期/Last Update: 2015-04-20