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玉米CMS-C不育系和保持系mads3基因克隆与表达分析(英文)(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2014年05期
页码:
675-680
栏目:
植物生理与分子生物学
出版日期:
2014-09-10

文章信息/Info

Title:
Cloning and expression analysis of mads3 in maize CMS-C sterile line and its maintainer line
文章编号:
1000-3142(2014)05-0675-06
作者:
王继玥 汪生庆 曹墨菊*
四川农业大学 玉米研究所/教育部作物基因资源与遗传改良重点实验室/农业部 西南玉米生物学及遗传育种重点实验室, 成都 611130
Author(s):
WANG Ji-Yue WANG Sheng-Qing CAO Mo-Ju*
Maize Research Institute of Sichuan Agricultural University/Key Laboratory of Crop Genetic Resource and Improvement, Ministry of Education/Key Laboratory of Maize Biology and Genetic Breeding on Southwest, Ministry of Agriculture, Chengdu 611130, China
关键词:
玉米 CMS-C 花药 mads3基因
Keywords:
maize CMS-C anther mads3
分类号:
Q943.2
DOI:
10.3969/j.issn.1000-3142.2014.05.017
文献标识码:
-
摘要:
MADS-box基因参与植物多种发育过程,尤其在花发育过程中发挥着重要的调控作用。该研究以玉米不育系C48-2及保持系N48-2为材料,采用RT-PCR克隆mads3基因。序列分析发现,在不育系C48-2中该基因编码区发生单碱基突变,导致其推定的蛋白质中5个氨基酸序列发生改变。荧光定量PCR显示,相对于保持系,花粉母细胞时期和四分体时期mads3基因在C48-2下调表达,而单核期和双核期上调表达。这些结果为进一步了解玉米C型细胞质雄性不育的发生机制提供参考。
Abstract:
MADS-box genes involve in various developmental processes in plant,and play an important role in the regulation of floral development. In this paper,mads3,a member of MADS box gene family was cloned by RT-PCR in maize. Sequence analysis showed that 8 sits single-base substitution mutations in CDS region were found in CMS-C line in comparison to maintainer line,which may result in 5 individual amino acides change. Furthermore,real-time qPCR analysis showed that mads3 was significantly down-regulated in CMS-C line at tetrad stage,and was significantly up-regulated at uninucleate stage. These results laid the foundation for further studying the relationship between MADS-box genes and CMS-C in maize.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期: 2013-12-28修回日期: 2014-03-31
基金项目: 国家自然科学基金(30971794)
作者简介: 王继玥(1984-),男,四川南充人,博士研究生,主要研究方向为玉米细胞质雄性不育,(E-mail)acute2803764 @163.com。 *通讯作者: 曹墨菊,博士,教授,主要研究方向为玉米生物技术育种,(E-mail)caomj@sicau.edu.cn。
更新日期/Last Update: 2014-09-10