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梵净山冷杉和元宝山冷杉的叶绿体微卫星遗传多样性分析(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2014年05期
页码:
596-600
栏目:
系统演化与生物多样性
出版日期:
2014-09-10

文章信息/Info

Title:
Genetic diversity at chloroplast microsatellites(cpSSR) in Abies fanjingshanensis and A. yuanbaoshanensis
文章编号:
1000-3142(2014)05-0596-05
作者:
韦 范1 2 张广荣1 覃永贤1 刘付永清1 唐绍清1*
1. 广西师范大学 生命科学学院, 珍稀濒危动植物生态与环境保护省部共建教育部重点实验室, 广西 桂林 541004; 2. 广西壮族自治区药用植物园, 南宁 530023
Author(s):
WEI Fan12 ZHANG Guang-Rong1 QIN Yong-Xian1 LIUFU Yong-Qing1 TANG Shao-Qing1*
1. Key Laboratory for Ecology of Rare and Endangered Species and Environmental Protection Co-established by Guangxi and the Ministry of Education, College of Life Sciences, Guangxi Normal University, Guilin 541004, China; 2. Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China
关键词:
梵净山冷杉 元宝山冷杉 叶绿体微卫星 遗传多样性
Keywords:
Abies fanjingshanensis A. yuanbaoshanensis chloroplast microsatellite genetic diversity
分类号:
Q948.12; Q16
DOI:
10.3969/j.issn.1000-3142.2014.05.004
文献标识码:
-
摘要:
梵净山冷杉和元宝山冷杉是极度濒危的国家一级重点保护植物。应用叶绿体微卫星标记(cpSSR)研究它们的遗传多样性,并与同属的广布种岷江冷杉的一个种群进行比较。结果表明:3对cpSSR引物(Pt63718、Pt30204和Pt71936)在这3种冷杉的249个个体中共检测到21个等位基因,组成35种单倍型; 它们的单倍型数和有效单倍型数分别为梵净山冷杉(No=12,Ne=3.92),元宝山冷杉(No=9,Ne=3.28),两者均低于广布种岷江冷杉(No=14,Ne=11.57); 梵净山冷杉和元宝山冷杉的稀有单倍型较少,其频率最高的单倍型分别出现在该种群的46.0%和44.1%的个体中; 梵净山冷杉(He=0.75)和元宝山冷杉(He=0.70)的单倍型多样性也低于岷江冷杉(He=0.97)。梵净山冷杉和元宝山冷杉的叶绿体微卫星遗传多样性水平低。
Abstract:
Abies fanjingshanensis and A. yuanbaoshanensis are critically endangered plants. We used chloroplast microsatellite marker to compare the genetic diversity of A. yuanbaoshanensis and A. fanjingshanensis with a widely distributed species A. fargesii var. faxoniana. A total of 21 alleles and 35 haplotypes was detected in 249 individuals from 3 pairs of primer(Pt63718,Pt30204 and Pt71936). The number of haplotypes(No),effective number of haplotypes(Ne)of A. fanjingshanensis(No=12,Ne=3.92)and A. yuanbaoshanensis(No=9,Ne=3.28)were lower than A. fargesii var. faxoniana. A. fanjingshanensis and A. yuanbaoshanensis had less rare haplotypes. The haplotype diversity(He)of A. fanjingshanensis(He=0.75)and A. yuanbaoshanensis(He=0.70)were also lower than A. fargesii var. faxoniana(He=0.97). A. fanjingshanensis and A. yuanbaoshanensis had a relatively low level of genetic diversity at chloroplast microsatellite.

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备注/Memo

备注/Memo:
收稿日期: 2013-11-30修回日期: 2013-12-25
基金项目: 国家自然科学基金(31060034,30760029)
作者简介: 韦范(1984-),女,广西南宁人,硕士,助理研究员,研究方向为植物种群遗传,(E-mail)fanfanmeister@gmail.com。 *通讯作者: 唐绍清,博士,教授,主要从事植物学教学和研究,(E-mail)shaoqing@mailbox.gxnu.edu.cn。
更新日期/Last Update: 2014-09-10