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倍性分析和AFLP标记相结合鉴定金柑新种质(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2014年04期
页码:
548-551
栏目:
系统演化与生物多样性
出版日期:
2014-07-20

文章信息/Info

Title:
Identification of Kumquats(Fortunella crassifolia) new varieties by ploidy analysis and AFLP
文章编号:
1000-3142(2014)04-0548-04
作者:
傅翠娜 肖远辉 甘海峰* 雷新南
广西特色 作物研究院, 广西 桂林 541004
Author(s):
FU Cui-Na XIAO Yuan-Hui GAN Hai-Feng* LEI Xin-Nan
Guangxi Academy of Sepcialty Crops, Guilin 541004, China
关键词:
金柑 新种质 倍性分析 遗传鉴定 AFLP
Keywords:
Kumquats new varieties ploidy analysis genetic identification AFLP
分类号:
S666.9
DOI:
10.3969/j.issn.1000-3142.2014.04.023
文献标识码:
A
摘要:
采用倍性分析和AFLP标记相结合,对滑皮金桔和变异单株金柑Ⅱ进行遗传鉴定分析。结果表明:滑皮金桔是2倍体,金柑Ⅱ是4倍体; 选用12对AFLP引物组合,就8份金柑资源进行遗传多样性分析及分类研究,共获得清晰可见的标记341,其中多态性标记95,占27.8%,供试材料相似系数在0.867~0.985之间,能将8个品种区分开,滑皮金桔与普通金柑相似性系数为96.8%,金柑Ⅱ与普通金柑相似性系数为95.8%,滑皮金桔与金柑Ⅱ相似性系数为95.7%。表明滑皮金桔和金柑Ⅱ是不同于普通金柑的变异新种质,可以直接作为品种资源加以利用。
Abstract:
To determine the genetic diversity of Kumquats new varieties. We employed ploidy analysis and 12 pairs of AFLP primer combinations on 8 Kumquats varieties. Ploidy analysis confirmed that Huapijingan was a diploid,Jingan Ⅱ was a tetraploid. AFLP results showed that: A total of 341 major AFLP bands were observed,and 95 band were polymorphism. Genetic similarities among all taxa ranged from 0.867 to 0.985. 8 varieties could be divided. Phenetic trees based on genetic similarities(UPGMA,N-J)were similar to known taxonomic relationships. the genetic similarity coefficient between Huapijingan and Jingan was 96.8%,the genetic similarity coefficient between Jingan Ⅱ and Jingan was 95.8%,the genetic similarity coefficient between Jingan Ⅱ and Huapijingan was 95.7%. Jingan Ⅱ and Huapijingan could be used as new cultivar.

参考文献/References

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Bernet GP,Asins MJ. 2003. Identification and genomic distribution of gypsy like retrotransposons in Citrus and Poncirus[J]. Theor Appl Genet,108(1):121-130
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Luo AC(罗安才). 2003. Researches on the organic acids metabolism in citrus fruits and the AFLP analysis of the Fengjie navel orange mutants(柑桔果实有机酸代谢生理和奉节脐橙芽变株系的AFLP分析研究)[D]. Chongqing(重庆):Southwest Agriculture University(西南农业大学):1-99
Mu HN(母洪娜),Zeng JW(曾继吾),Yi GJ(易干军),et al. 2007. A simple and effective DNA extraction method for AFLP analysis citrus(一种快速高效适于柑桔AFLP分析的DNA提取方法)[J]. Guangdong Agric Sci(广东农业科学),(3): 25-26
Peng HX(彭宏祥),Li DB(李冬波),Zhu JH(朱建华),et al. 2008. Genetic diversity of longan in Guangxi assessed by AFLP markers(用AFLP标记分析广西龙眼种质遗传多样性)[J]. Acta Hortic Sin(园艺学报),35(10):1 511-1 516
Xiao YH(肖远辉),Mai SQ(麦适秋),Fu CN(傅翠娜),et al. 2012. Identification of shatianyou pummelos(Citrus grandis)new varieties by AFLP(利用AFLP鉴定沙田柚新种质)[J]. J Southern Agric(南方农业学报),43(11):1 642-1 645
Yi GJ(易干军),Yu XY(余晓英),Huo HQ(霍合强),et al. 2002. Studies on the certification of Musa ABB group Dajiao,M. AAB group Fenjiao and M. AAB group Longyajiao by AFLP(粉蕉、大蕉和龙牙蕉的AFLP分类研究)[J]. Acta Hortic Sin(园艺学报),29(5):413-417
Yi GJ(易干军),Huo HQ(霍合强),Chen DC(陈大成),et al. 2003. Studies on genetic relationship among litchi varieties by using AFLP(荔枝品种亲缘关系的AFLP分析)[J]. Acta Hortic Sin(园艺学报),30(4):399-403
Yi GJ(易干军),Tan WP(谭卫萍),Huo HQ(霍合强),et al. 2003. Studies on the genetic diversity and relationship of longan cultivars by AFLP analysis(龙眼品种(系)遗传多样性及亲缘关系的AFLP分析)[J]. Acta Hortic Sin(园艺学报),30(3):272-276
Zabeau M,et al. 1993. Eurpean Patent[P]. 0535858A1,(3):31
Zhang JE(张俊娥),Liu JH(刘继红),Deng XX(邓秀新),et al.2003. Genetic variation of citrus calli revealed by the ploidy analyser(采用倍性分析仪鉴定柑桔愈伤组织的遗传变异)[J]. Acta Genet Sin(遗传学报),30(2):169-174

备注/Memo

备注/Memo:
收稿日期: 2014-01-09
修回日期: 2014-03-19
基金项目: 广西自然科学基金(桂科自0991186); 广西科技攻关项目(桂科攻1123003-3D)。
作者简介: 傅翠娜(1979-),女,山东莱州人,硕士,助理研究员,主要从事柑桔栽培、育种方面的研究,(E-mail)fucuina99@163.com。 *通讯作者: 甘海峰,研究员,主要从事柑桔栽培技术研究工作,(E-mail)ganhf6919@126.com。
更新日期/Last Update: 2014-07-20