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大孔树脂对甜菊糖的纯化(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2014年04期
页码:
525-529
栏目:
植物化学与化学生物学
出版日期:
2014-07-20

文章信息/Info

Title:
Purification of stevioside ST and RA by macroporous resin
文章编号:
1000-3142(2014)04-0525-05
作者:
阳小宇1 陈志良2 郝再彬2*
1. 湖南化工职业技术学院, 湖南 株洲 412004; 2. 桂林理工大学 化学与生物工程学院, 广西 桂林 541004
Author(s):
YANG Xiao-Yu1 CHEN Zhi-Liang2 HAO Zai-Bin2*
1. Hunan Chemical Industry Professional and Technology College, Zhuzhou 414002, China; 2. Chemical and Biological Engineering College, Guilin University of Technology, Guilin 541004, China
关键词:
大孔树脂 莱鲍迪甙A 甜菊甙 分离纯化 高效液相色谱
Keywords:
macroporous resin rebaudioside A stevioside purification HPLC
分类号:
TQ461
DOI:
10.3969/j.issn.1000-3142.2014.04.019
文献标识码:
A
摘要:
通过静态吸附比较三种大孔树脂对甜菊糖中莱鲍迪甙A(RA)和甜菊甙(ST)的吸附和解吸能力,利用高效液相色谱对结果进行检测,从中筛选出对RA和ST吸附效果最佳的LX-68M树脂。纯化工艺为30 ℃下,LX-68M树脂对RA和ST在6 h后达到吸附饱和,吸附量分别为干树脂RA 85.00 mg/g和ST 121.50 mg/g。动态洗脱结果表明:以55%的乙醇为洗脱液,在2 BV/h的洗脱速度下,经LX-68M树脂处理后的甜菊糖溶液纯度由75.5%提高到77.8%。表明LX-68M树脂对于甜叶菊糖苷工业生产的应用前景较好。
Abstract:
The adsorption and desorption capacities of three kinds of macroporous resins for RA and ST contained in stevioside were investigated through static adsorption experiment. The adsorption and desorption capacities was quantified using high performance liquid chromatography(HPLC),and the results indicated that the LX-68M had the best adsorption and desorption capacities. Under 30 ℃,the absorption equilibrium of LX-68M resin was achieved for RA(85.0 mg/g dry resin)and ST(121.5 mg/g dry resin)after 6 h,respectively. The dynamic desorption was carried out under 2 BV/h eluting employing 55%(V%)ethanol/water as eluent,and the results showed that the purity of stevia glycosides increased from 75.5% to 77.8%. On the basis of these findings,it was believed that the LX-68M resin had industrial application promise for stevia glycosides.

参考文献/References

Aehle E,Grandic SZ,Ralainirina R. 2004. Development and evaluation of an enriched natural antioxidant preparation obtained from aqueous pinach(spinaciaoleracea)extracts by an adsorption procedure[J]. Food Chem,(86):579-585
Bouzid O,Navarro D,Roche M. 2005. Fungal enzymes as a powerful tool to release simple phenolic compounds from olive oil by-product[J]. Proc Biochem,(40):1 855-1 862
Chen TH(陈天红),Zhang Y(张杨),Liu XH(刘晓航). 1998. Selective research of stevioside composition by high polymer adsorbent(高分子吸附剂对甜叶菊中各糖甙吸附选择性的研究)[J]. Sci Chin(中国科学),2(28):460-465
Ding N(丁宁),Hao ZB(郝再彬). 2005. Research and development of stevia glycosides(甜叶菊及其糖苷的研究与发展)[J]. Shanghai J Agric Sci & Technol(上海农业科技),(4):8-10
Hu J(胡静),Chen YR(陈育如),Wei X(魏霞)2008. Separation of stevioside SS and RA bymacroporous Resins D107 and D108(大孔树脂D107和D108对甜菊糖中SS和R A 的分离研究)[J]. Food Res & Dev(食品研究与开发),29(6):1-4
Hu XL(胡献丽),Dong WB(董文宾),Zheng D(郑丹). 2005. 甜菊及甜菊糖研究进展[J]. Food Res & Dev(食品研究与开发),26(1):36-38
Liu C(刘超),Li LS(李来生),Xu LL(许丽丽). 2007. HPLC analysis of RA and ST in stevioside(高效液相色谱法测定甜叶菊糖中的甜菊苷和莱鲍迪苷A)[J]. Chin J Anal Lab(分析实验室),26(7):23-26
Liu JL(刘金磊),Chen SC(陈思呈),Lu FL(卢凤来). 2012. 番茄化学成分的分离与鉴定[J]. Guihaia(广西植物),32(3):415-418
Li N(李娜),Chen Z(陈智),Tian JZ(田景振). 2011. Optimization of purification of stevioside by macroporous adsorption resins(优选大孔树脂纯化甜菊苷的研究)[J]. Food & Drug(食品与药品),13(3):107-110
Luo YL(罗艳玲),Ou SY(欧仕益). 2005. Application of macroporous resin in separation of active components in food industry(大孔树脂在食品活性成分分离中的应用)[J]. Food & Mech(食品与机械),21(5):177-179
Teng XJ(滕祥金),Yang D(杨丹). 2007. On assayed stevioside and rebaudioside A by TLC(薄板层析法分析甜叶菊糖苷)[J]. Sug Crops Chin(中国糖科),(4):24-26
Wang YF(王一凡),Ren D(任岱),Zhou FX(周凤贤). 1997. Research of stevia nutrients in food(甜菊糖食品中营养成分的研究)[J]. China J Mod Med(中国现代医学杂志),7(3):63-64
Wang R(王瑞). 2008. HPLC analysis of stevioside(法测定甜菊糖甙)[J]. J Liaoning Univ(辽宁大学学报),35(4):355-357
Yang D(杨丹),Hao ZB(郝再彬). 2005. Determination of stevioside by flow injection chemiluminescence(流动注射化学发光法测定甜叶菊糖苷)[J]. Chem Eng(化学工程师),(4):23-24
Zhao XH(赵兴华),Zhang QX(张全香),Yuan XW(苑晓威). 2012. Purification technology of steviol glycosides by macroporous adsorption resin(大孔吸附树脂纯化甜菊糖苷的工艺研究)[J]. Chin Trad Herb Drugs(中草药),43(10):1 971-1 974
Zhang Z(张志),Teng XJ(滕祥金),Hao ZB(郝再彬). 2009. On assayed stevioside and rebaudioside A by TLC(薄板层析法分析甜叶菊糖苷)[J]. Chin Cond(中国调味品),34(3):94-96 Rasmussen HN. 1992. Seed dormancy patterns in Epipactis palustris(Orchidaceae):requirements for germination and establishment of ycorrhiza[J]. Physiol Plantarum,86(1):161-167
Sebastinraj J,Britto JS,Robinson PJ,et al. 2006. In vitro seed germination and plantlet regeneration of Coelogyne mossiae Rolfe[J]. J Biol Res,5:79-84
Seeni S,Latha PG. 1992. Foliar regenetation of the endangered Red Vanda,Renanthera imschootiana Rolfe(Orchidaceae)[J]. Plant Cell Tiss Org,29(3):167-172
Song XM(宋晓明),Liu G(刘光),Li QF(万群芳). 2011. Study on artificial propagation of Spiranthes sinensis(兰科植物盘龙参的人工繁育技术研究)[J]. Chin New Technol Prod(中国新技术新产品),3:359-360
Thompson DI,Edwards TJ,Staden JV. 2006. Evaluating asymbiotic seed culture methods and establishing Disa (Orchidaceae)germinability in vitro:relationships,requirements and first-time reports[J]. Plant Growth Regul,49(2-3):269-284
Zhang W(张伟),Jin CS(金传山),Zhou YW(周亚伟). 2010. Research advances on Spiranthes sinensis(盘龙参研究进展)[J]. Anhui Med Pharm J(安徽医药),14(7):748-750
Zhou XL(周秀玲),Li JM(李家敏). 2012. Preliminary studies on shapes,germination and storage of Spiranthes Sinensis seeds(药用植物盘龙参种子形态、萌发及贮藏的初步研究)[J]. Seed(种子),31(6):79-82
Znaniecka J,Eojkowska E. 2004. Establishment of in vitro culture collection of endangered European orchids[J]. Bull Bot Gard,13:69-73

备注/Memo

备注/Memo:
收稿日期: 2013-09-21
修回日期: 2013-10-13
基金项目: 湖南省高等学校科研项目( 11C0465); 湖南省科技厅项目(2012SK3180)。
作者简介: 阳小宇(1964-),女,硕士,高级实验师,从事功能材料制备与应用,(E-mail)chengzhiliang0411@163.com。 *通讯作者: 郝再彬,教授,博士生导师,主要从事生物活性物质的开发与利用,(E-mail)hao1173042@163.com。
更新日期/Last Update: 2014-07-20