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大叶桉叶水浸提液成分分析(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2014年04期
页码:
520-524
栏目:
植物化学与化学生物学
出版日期:
2014-07-20

文章信息/Info

Title:
Composition analysis on water leaching solution of Eucalyptus robusta leaf
文章编号:
1000-3142(2014)04-0520-05
作者:
李 群1 谭韵雅1 王 平3 魏 琴2* 钱 双2 石 丹2
1. 四川师范大学 生命科学学院, 成都 610101; 2. 宜宾学院发酵资源与应用四川省 高校重点实验室, 四川 宜宾 644000; 3. 四川省简阳中学, 四川 简阳 641400
Author(s):
LI Qun1 TAN Yun-Ya1 WANG Ping3 WEI Qin 2*QIAN Shuang 2 SHI Dan2
1. College of Life Sciences,Sichuan Normal University, Chengdu 610101, China; 2. Key Laboratory of Yibin College Fermentation Resources Universities of Sichuan, Yibin 644000, China; 3. Sichuan Jianyang Midde School, Jianyang 641400, China
关键词:
大叶桉 水浸提液 GC-MS 成分分析
Keywords:
Eucalyptus robusta water leaching solution GC-MS component analysis
分类号:
Q949.772
DOI:
10.3969/j.issn.1000-3142.2014.04.018
文献标识码:
A
摘要:
为进一步明确大叶桉的化学成分,对大叶桉叶水浸提液分别用不同极性的有机溶剂石油醚、乙酸乙酯和正丁醇进行萃取,对各萃取相进行GC-MS分析。结果表明:大叶桉叶水浸提液共含有37种化合物,其中,石油醚萃取相中含有20种,主成分为草酸丁基异己酯(37.24%); 乙酸乙酯萃取相中含有16种,主成分为2,2-二亚甲基双[6-(1,1-二甲基乙基-4-甲基)]-苯酚(50.05%); 正丁醇萃取相中含有5种,主成分为丙基-2-甲基丁酸酯(54.57%)。在所有成分中,酯类物质居多,也有少量的烯、酮、醇、苯和烷烃。1-甲基,4-(1-甲基乙基)-1,4环己二烯、2,2-二亚甲基[6-(1,1-二甲基乙基)-4-甲基]苯酚、1-十八烯和二十烷为石油醚和乙酸乙酯的共有成分; 1、2-苯二甲酸单(2 -乙基己基)酯为乙酸乙酯和正丁醇的共有成分。 该研究进一步明确了大叶桉的化学成分,为其在医药、化工和化感方面的应用研究奠定了基础。
Abstract:
With different polarity organic solvent extracted water leaching solution of Eucalyptus robusta leaf and analyzed by gas chromatography-mass spectrometry(GC-MS)in order to make a clear definition of the chemical components. 37 compounds were identified. Among them,the petroleum ether extraction phase contained 20 kinds and main component was oxalic acid,butyl isohexyl ester(37.24%); Ethyl acetate extraction phase contained 16 kinds and main component was phenol,2,2’-methylenebis[6-(1,1-dimethylethyl)]-4-methyl-(50.05%); N-butanol extraction phase contains 5 kinds and main component was butanoic acid,2-methylpropyl ester(54.57%). Of all the ingredients,ester material in the majority,also had a small amount of olefin,ketones,alcohols,benzene and alkane. 1,4-Cyclohexadiene,1-methyl-4-(1-methylethyl)-,phenol,2’-methylenebis[6-(1,1-dimethylethyl)-4-methyl-,1-octadecene and eicosane for petroleum ether and ethyl acetate common composition,1,2-benzenedicarboxylic acid,mono(2-ethylhexyl)ester for ethyl acetate and n-butanol common composition. The study further defined the chemical components of E. robusta and builted the foundation for its application in pharmaceutical,chemical and allelopathy.With different polarity organic solvent extracted water leaching solution of Eucalyptus robusta leaf and analyzed by gas chromatography-mass spectrometry(GC-MS)in order to make a clear definition of the chemical components. 37 compounds were identified. Among them,the petroleum ether extraction phase contained 20 kinds and main component was oxalic acid,butyl isohexyl ester(37.24%); Ethyl acetate extraction phase contained 16 kinds and main component was phenol,2,2’-methylenebis[6-(1,1-dimethylethyl)]-4-methyl-(50.05%); N-butanol extraction phase contains 5 kinds and main component was butanoic acid,2-methylpropyl ester(54.57%). Of all the ingredients,ester material in the majority,also had a small amount of olefin,ketones,alcohols,benzene and alkane. 1,4-Cyclohexadiene,1-methyl-4-(1-methylethyl)-,phenol,2’-methylenebis[6-(1,1-dimethylethyl)-4-methyl-,1-octadecene and eicosane for petroleum ether and ethyl acetate common composition,1,2-benzenedicarboxylic acid,mono(2-ethylhexyl)ester for ethyl acetate and n-butanol common composition. The study further defined the chemical components of E. robusta and builted the foundation for its application in pharmaceutical,chemical and allelopathy.

参考文献/References

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Tang WZ(唐伟军),Zhou JF(周菊峰),Li XN(李晓宁),et al. 2006. Study on the chemical components in leaf essential oil of Eucalyptus robusta(大叶桉叶挥发油的化学成分研究)[J]. J Anal Sci(分析科学学报),22(2):182-186
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Tian YH(田玉红),Liu XM(刘雄民),Zhou YH(周永红),et al. 2007. Chemical compositions of essential oil of Eucalyptus umbellate growing in Guangxi(圆角桉叶精油的化学成分)[J]. Food Sci(食品科学),28(1):36-38
Wang HG(王晗光),Zhang J(张健),Yang WS(杨婉身),et al. 2006. Analysis of volatile allelopathic constituents of leaf of Eucalyptus grandis by gas chromatography-mass spectrometry(气相色谱-质谱法分析巨桉叶的挥发性化感成分)[J]. J Sichuan Agric Univ(四川农业大学学报),24(1): 51-54
Wang HG(王晗光),Zhang J(张健),Yang WS(杨婉身),et al. 2006. Report of three different extractions on the allelopathic substances in root of Eucalyptus grandis(3种方法提取巨桉树根的化感成分分析报告)[J]. J Hebei Norm Univ:Nat Sci Edit(河北师范大学学报·自然科学版),30(4):464-467,477
Wang J(王静),Wang L(王磊),Li YQ(李永青),et al. 2013. Study on herbicidal activity of three Eucalypt leaf extracts(3种桉树叶提取物除草活性研究)[J]. Chin Agric Sci Bull(中国农学通报),29(6):191-194
Yin QH(殷清华),Liang ZY(梁振益),Lin ZH(林昭华),et al. 2008. revision of the bloodwoods genus Corymbia(Myrtaceae)(桉树叶挥发油化学成分的研究)[J]. Telopea (化学分析计量),17(3):30-31
Zhu YL(朱宇林),Tan P(谭萍),Lu SF(陆绍锋),et al. 2011. Bioassay of allelopathic activity of water extract of Eucalyptus leaves on seed germination of different kinds of plants(桉树叶水浸提液对4种植物种子化感作用的生物测定)[J]. J Northwest For Univ(西北林学院学报)26(1):134-137
Zhang D,Zhang J,Yang W,et al. 2010. Potential allelopathic effect of Eucalyptus grandis across a range of plantation ages[J]. Ecol Res,25(1):13-23Ahmed R,Hoque ATMR,Hossain MK. 2008.
Allelopathic effects of leaf litters of Eucalyptus camaldulensis on some forest and agricultural crops[J]. J For Res,19(1):19-24
Chen HX(陈虹霞),Wang CZ(王成章). 2010. Chemical compositions of essential oil of four Eucalyptus leaves from africa(4种非洲桉树叶挥发油的化学成分研究)[J]. Biomass Chem Engin(生物质化学工程),44(6):23-27
Chen QB(陈秋波),He LM(贺利民),Yuan HQ(袁洪球),et al. 2004. Identification and quantitation of allelochemicals from fresh leaves of Eucalyptus ABL12(刚果12号桉叶化感物质的初步分离分析)[J]. Chin J Trop Crop(热带作物学报),25(4):84-91
Chen YY(陈月圆),Lu FL(卢凤来),Li DB(李典鹏),et al. 2010. Analysis of volatile constituents of different types of Eucalyptus leaf by GC-MS(不同品种桉树叶挥发性成分的GC-MS分析)[J]. Guihaia(广西植物),30(6):895-898
Fang B,Yu S,Wang Y,et al. 2009. Allelopathic effects of Eucalyptus urophylla on ten tree species in south China[J]. Agroforestry systems,76(2):401-408
He YS(何耀松),Zhang JD(张继东). 2007. Progress on chemical constituents and pharmacologi-cal effects of Eucalyptus(桉树化学成分及药理作用研究进展)[J]. Prog Veter Med(动物医学进展),28(7):98-101
HillK D,Johnson LAS. 1995. Systematic studies in the eucalyptus. A revision of the bloodwoods genus Corymbia(Myrtaceae)[J]. Telopea,6(2/3):185-504
Huang BS(黄炳生),Zhou XL(周晓农),Zhu D(朱丹),et al. 2012. Study on the chemical constituents of essential oil from the leaf of Eucalyptus urophylla in Guangdong(广东产尾巨桉叶挥发油化学成分的GC-MS分析)[J]. J Chin Med Mat(中药材),35(8):1 268-1 270
Huang Y(黄瑶),Tian YH(田玉红),Liu XM(刘雄民),et al. 2010. Study on the composition and its antimicrobial effects of essential oils from leaves of Eucalyptus urophylla(尾叶桉叶精油的成分分析及抑菌效果初探)[J]. Northern Hort(北方园艺),(6):26-28
Lan MB(兰美兵),Li XH(李啸红),Yu YL(余永莉),et al. 2012. Study on chemical constituents and genotoxicity of volatile oil in folium Eucalyptus robusta from Guangdong(广东产大叶桉叶挥发油的化学成分和遗传毒理学研究)[J]. Pharmac Clin Chin Mat Med(中药药理与临床),28(6):82-85
Liu X,Chen Q,Wang Z,et al. 2008. Allelopathic effects of essential oil from Eucalyptus grandis�/i>215;E. urophylla on pathogenic fungi and pest insects[J]. Front For Chin,3(2):232-236
Liu XX(刘小香),Xie LL(谢龙莲),Chen QB(陈秋波),et al. 2004. A review of allelopathic researches on Eucalyptus(桉树化感作用研究进展)[J]. Chin J Trop Agric(热带农业科学),24(2):54-61
Liu YM(刘玉明),Chai YF(柴逸锋),Wu YT(吴玉田),et al. 2004. Study on GC-MS analysis of volatile oil constituents from Eucalyptus globulus fruit and Eucalyptus robusta fruit(GC-MS对蓝桉果实及大叶桉果实挥发油成分研究)[J]. Chin J Pharmac Anal(药物分析杂志),24(1):24-26
Qi JH(戚建华),Liang YL(梁银丽),Liang ZS(梁宗锁). 2004. The review of researching Allelopathy in agro-ecosystem(农业生态系统中化感作用研究综述)[J]. Acta Agric Bor-Occid Sin(西北农业学报),13(2):115-118
Song AH(宋爱华),Wang Y(王颖),Liu YM(刘艳梅). 2009. Study on GC-MS analysis of volatile oil constituents from Eucalyptus globulus labill leaf(蓝桉叶挥发油化学成分的气相色谱-质谱分析研究)[J]. Food & Drug(食品与药品),11(1):31-32
Tang WZ(唐伟军),Zhou JF(周菊峰),Li XN(李晓宁),et al. 2006. Study on the chemical components in leaf essential oil of Eucalyptus robusta(大叶桉叶挥发油的化学成分研究)[J]. J Anal Sci(分析科学学报),22(2):182-186
Tan RX(谭仁祥). 2003. plant functional constituents(植物成分功能)[M]. Beijing(北京):Science Press(科学出版社):35-60
Tian YH(田玉红),Liu XM(刘雄民),Zhou YH(周永红),et al. 2007. Chemical compositions of essential oil of Eucalyptus umbellate growing in Guangxi(圆角桉叶精油的化学成分)[J]. Food Sci(食品科学),28(1):36-38
Wang HG(王晗光),Zhang J(张健),Yang WS(杨婉身),et al. 2006. Analysis of volatile allelopathic constituents of leaf of Eucalyptus grandis by gas chromatography-mass spectrometry(气相色谱-质谱法分析巨桉叶的挥发性化感成分)[J]. J Sichuan Agric Univ(四川农业大学学报),24(1): 51-54
Wang HG(王晗光),Zhang J(张健),Yang WS(杨婉身),et al. 2006. Report of three different extractions on the allelopathic substances in root of Eucalyptus grandis(3种方法提取巨桉树根的化感成分分析报告)[J]. J Hebei Norm Univ:Nat Sci Edit(河北师范大学学报·自然科学版),30(4):464-467,477
Wang J(王静),Wang L(王磊),Li YQ(李永青),et al. 2013. Study on herbicidal activity of three Eucalypt leaf extracts(3种桉树叶提取物除草活性研究)[J]. Chin Agric Sci Bull(中国农学通报),29(6):191-194
Yin QH(殷清华),Liang ZY(梁振益),Lin ZH(林昭华),et al. 2008. revision of the bloodwoods genus Corymbia(Myrtaceae)(桉树叶挥发油化学成分的研究)[J]. Telopea (化学分析计量),17(3):30-31
Zhu YL(朱宇林),Tan P(谭萍),Lu SF(陆绍锋),et al. 2011. Bioassay of allelopathic activity of water extract of Eucalyptus leaves on seed germination of different kinds of plants(桉树叶水浸提液对4种植物种子化感作用的生物测定)[J]. J Northwest For Univ(西北林学院学报)26(1):134-137
Zhang D,Zhang J,Yang W,et al. 2010. Potential allelopathic effect of Eucalyptus grandis across a range of plantation ages[J]. Ecol Res,25(1):13-23

备注/Memo

备注/Memo:
收稿日期: 2013-11-29
修回日期: 2014-01-29
基金项目: 四川省教育厅项目(08ZA002); 四川省科技计划项目(2010JY0093); 四川省青年科技创新研究团队项目(2011JTD0035); 宜宾市科技局项目(2011ZSF008)。
作者简介: 李群,女,重庆秀山人,博士,副教授,从事资源植物学领域研究,(E-mail)liqun01234@163.com。 *通讯作者: 魏琴,博士,教授,从事资源植物学领域研究,(E-mail)weiqin2001-67@163.com。
更新日期/Last Update: 2014-07-20