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广西五种绞股蓝属植物离体快繁与种质保存研究(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2014年04期
页码:
436-441
栏目:
植物生理与分子生物学
出版日期:
2014-07-20

文章信息/Info

Title:
Study on rapid propagation and germplasm conservation in vitro of five species of genus Gynostemma in Guangxi
文章编号:
1000-3142(201
作者:
姚绍嫦1 谢月英12 黄雪彦12 余丽莹12*
1. 广西药用植物园, 南宁 530023; 2. 广西药用资源保护与遗传改良重点实验室, 南宁 530023
Author(s):
YAO Shao-Chang1 XIE Yue-Ying12 HUANG Xue-Yan12 YU Li-Ying12*
1. Guangxi Botanical Garden of Medicinal Plant, Nanning 530023, China; 2. Guangxi key Laboratory of medicinal Resources conservation and Genetic Improvement, Nanning 530023, China
关键词:
绞股蓝 扁果绞股蓝 广西绞股蓝 光叶绞股蓝 长梗绞股蓝 组织培养 离体保存
Keywords:
Gynostemma pentaphyllum G. guangxiense G. compressum G. laxum G. longipes tissues culture conservation in vitro
分类号:
Q943.1
DOI:
10.3969/j.issn.1000-3142.2014.04.0021000
文献标识码:
A
摘要:
以绞股蓝属植物的带芽茎段为材料,研究不同6-BA浓度与NAA 0.02 mg·L-1组合对其诱导、分化和增殖的影响,并建立离体快繁体系。结果表明:MS+6-BA 2.0 mg·L-1+NAA 0.02 mg·L-1最适宜初代诱导,MS+6-BA 2.0 mg·L-1+NAA 0.02 mg·L-1最适合扁果绞股蓝的增殖培养,而MS+6-BA 1.5 mg·L-1+NAA 0.02 mg·L-1是其它四种植物增殖的最佳培养基,在1/2MS+NAA 1. 0 mg·L-1上的生根率均达100%。1/2MS与蔗糖40 g·L-1对五种植物的保存效果均最好; 添加生长抑制剂能有效减缓生长速度,最佳生长抑制剂为ABA和CCC,浓度均为1.0 mg·L-1,其中CCC能适合多个物种,连续保存360 d的存活率均在94.5%以上; PP333不适合五种植物的保存。活力检测表明,各种质经保存后增殖、生根能力均未下降。
Abstract:
Using stem with buds as explants,experiments were conducted to observe the effects of different concentration 6-BA and NAA 0.02 mg·L-1on induction,differentiation,proliferation of Gynostemma in Guangxi,in order to establish their rapid propagation systems. Meanwhile,different concentrations of the mineral nutrition,sucrose and plant grow inhibitors(CCC,ABA,PP333)were researched. The results showed that the best induction medium was MS medium supplemented with 2.0 mg·L-16-BA and 0.02 mg·L-1NAA. The suitable proliferation medium for G. compressum was MS+6-BA 2.0 mg·L-1+NAA 0.02 mg·L-1 and MS medium supplemented with 1.5 mg·L-16-BA and 0.2 mg·L-1NAA was best for others. The rooting medium was 1/2MS medium with 0.02 mg·L-1 NAA,accounting for 100% of rooting rate. 1/2MS medium and sucrose 40 g·L-1 were the most suitable for the conservation in vitro of all species. Plant grow inhibitors could slow down the grow speed effectively,and the best kind and concentration were ABA 1.0 mg·L-1and CCC 1.0 mg·L-1. Several species were inhibited by CCC 1.0 mg·L-1and the survival rate was up to 94.5% after being conserved 360 d. PP333 was not suitable for all species to conserve in vitro. Vigor testing showed that the ability of propagating and rooting of the plantlets didn’t fall down.

参考文献/References

Chang CK,Chang KS,Lin YC,et al. 2005. Hairy root cultures of Gynostemma pentanhyllum(Thunb.)Makino:a promising approach for the production of gypenosides as an alternative of ginseng saponins[J]. Biotechnol Letters,27:1 165-1 169
Chen XX(陈秀香),Liang DR(梁定仁). 1993. Ecological distribution and resources utilization of genus gynostemma in Guangxi(广西绞股蓝属的生态分布及资源利用)[J]. Bot J South Chin(华南植物学报),试刊:31-33
Fu CM(付传明),Huang NZ(黄宁珍),Zhao ZG(赵志国),et al. 2007. Study on preservation of Stephanie kwangsiensis H. S. Lo in vitro(广西地不容种质离体保存技术研究)[J]. Guangxi Sci(广西科学),14(2):155-159
Kartha KK,Mroginski LA,Pahl KK,et al. 1981. Germplasm preservation of coffea(Coffea arabica L.)by in vitro cuture of shoot apical meristems[J]. Plant Sci Letters,22:301-307
Li F(李锋),Fu CM(付传明),Huang NZ(黄宁珍). 2008. Study on preservation in vitro of Morinda officinalis(巴戟天种质离体保存研究)[J]. Guihaia(广西植物),28(1):95-99
Liu MH(刘敏华),Liu GZ(刘桂芝),Liu Y(刘影). 2001. Gynostemma pentaphyllum(绞股蓝)[M]. Beijing(北京):China Press of Traditional Chinese Medicine(中国中医药出版社):25
Liu SB(刘世彪),Li XY(李馨芸),Long H(龙华),et al. 2012. Tissue culture and rapid propagation of Gynostemma guangxiense(广西绞股蓝的组织培养和快速繁殖)[J]. Amino Acids & Biotic Resour(氨基酸和生物资源),34(1):29-32
Liu SB(刘世彪),Tao M(陶民),Jiang YF(姜业芳),et al. 2007. Tissue culture and rapid propagation of Gynostemma pentagynum Z. P. Wang(五柱绞股蓝的组织培养和快速繁殖)[J]. Plant Physiol Commun(植物生理学通讯),43(2):308
Liu SQ(刘松青),Wu CR(武成荣). 2000. Shoot tips culture and plants regeneration in Gynostemma pentaphyllum(Thunb.)Makino in vitro(绞股蓝茎尖组织培养和植株再生研究)[J]. Chin Wild Plant Resour(中国野生植物资源),19(6):60-62
Pan RZ(潘瑞炽),Dong YD(董愚得). 1995. Phytophysiology(植物生理学)[M]. 3rd edition(第3版). Beijing(北京):Higher Education Press(高等教育出版社):225-233 Pan RZ(潘瑞炽),Ye QS(叶庆生). 2006. The Physiology of Cymbidium(国兰生理)[M]. Beijing(北京):Science Press(科学出版社):103-104
Samer M,Neal MD,Basil DR. 2006. Anti-hyperlipidemic and hypoglycemic effects of gynostemma pentaphyllum in the zucker fatty rat[J]. J Pharm Pharmac Sci,9(3):281
Shang LS,L iu JC,Zhu QJ,et al. 2006. Gypenosides protect primary cultures of rat cortical cells against oxidative neurotoxicity[J]. Brain Res,11(2):163
Shi YZ(史永忠),Pan RC(潘瑞炽),Wang XJ(王小菁),et al. 1999. In vitro conservation of germplasm at room temperature in Dendrobium officinale(铁皮石解种质室温离体保存)[J]. J South Chin Norm Univer:Nat Sci Edit(华南师范大学学报·自然科学版),(4):73-77
Yang MH(杨明辉),Guo XL(郭晓兰),Yuan GH(袁国华),et al. 2006. Gypenosides induce apoptosis in human hepatoma cells(绞股蓝总皂甙对肝细胞瘤细胞凋亡的诱导作用)[J]. World Sci Technol Modern Trad Chin Med Mat Med(世界科学技术:中医药现代化),8(4):53
Wu F(吴峰),Gao W(高文). 2005. The optimization of medium of the tissue culture and rapid propagation of Gynostemma pentaphyllum(绞股蓝组培快繁培养基优化)[J]. Chin Agric Sci Bull(中国农学通报),21(7):70-72
Wu ZY,Peter HR. 2011. Flora of China[M]. Missouri Botanical Garden Press,19:11-15
Zhou X(周逊),Xiang CP(向长萍). 2008. Advance in slow growth conservation in vitro of plant(植物种质资源缓慢生长离体保存研究进展)[J]. Chin Veget(中国蔬菜),(11):39-42

备注/Memo

备注/Memo:
收稿日期: 2013-10-14
修回日期: 2013-12-08
基金项目: 广西科技攻关项目(桂科攻11107010-3-3)
作者简介: 姚绍嫦(1980-),女,广西容县人,硕士,高级工程师,主要从事药用植物组织培养及离体保存技术研究,(E-mail)yaoshaochang@163.com。 *通讯作者: 余丽莹,研究员,主要从事中药资源与物种保育研究,(E-mail)yuliying@vip.sina.com。
更新日期/Last Update: 2014-07-20