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|本期目录/Table of Contents|

螺旋藻有效成分超临界萃取与分离研究(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2014年03期
页码:
414-418
栏目:
植物化学与化学生物学
出版日期:
2014-07-02

文章信息/Info

Title:
Study on the effective constituents of Spirulina platensis with supercritical fluid extraction and separation
作者:
晏荣军 林 溪 胡佳扬 裘俊红 陆向红
浙江工业大学 化学工程与材料学院, 杭州 310014
Author(s):
YAN Rong-Jun LIN Xi HU Jia-Yang QIU Jun-Hong LU Xiang-Hong
College of Chemical Engineering and Materials, Zhejiang University of Technology, Hangzhou 310014, China
关键词:
螺旋藻 超临界CO2萃取 蛋白质 多糖
Keywords:
Spirulina platensis supercritical CO2 extraction protein polysaccharide
分类号:
Q946.3
DOI:
-
文献标识码:
A
摘要:
使用超临界CO2萃取技术对钝顶螺旋藻粉进行萃取与分离,结果表明:超临界CO2萃取的最优条件为压力20 Mpa,夹带剂使用量100 mL/100 g,温度50 ℃,萃取时间2 h; 利用凯氏定氮法及气相色谱法分别对超临界萃取处理过的螺旋藻中蛋白质和多糖进行研究与分析,结果表明:螺旋藻粉中蛋白质含量为49.39%,螺旋藻多糖中的单糖含量占多糖粗品的9.25%,主要组成为鼠李糖、木糖、甘露糖、葡萄糖、半乳糖等。
Abstract:
The effective constituents of the Spirulina platensis components were analysed and extracted used the supercritical CO2 extraction. The optimal conditions of supercritical CO2 were as follows: 20 MPa as extraction pressure,50 ℃ as extraction temperature,100 mL/100 g as entrainer,2 h as extraction time. And also analysis on Spirulina protein and polysaccharide by using Kjeldahl method and Gas Chromatography,the results showed that the protein content of Spirulina platensis was 49.39%,the content of Spirulina platensis monosaccharides in the polysaccharide was 9.25%. Most of monosaccharides was composed from rhamnose,xylose,mannose,glucose and galactose.

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备注/Memo

备注/Memo:
收稿日期: 2013-11-09修回日期: 2014-01-13
基金项目: 浙江省大学生新苗计划项目(2012R403044); 教育部留学回国人员科研启动基金。
作者简介: 晏荣军(1976-),男,湖南岳阳人,博士后,讲师,从事水环境生物学研究,(E-mail)yanrj@zjut.edu.cn。
更新日期/Last Update: 2014-05-25