我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

鸭梨PPO与GFP融合基因的烟草转化及亚细胞定位观察(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2014年03期
页码:
369-374
栏目:
植物生理与分子生物学
出版日期:
2014-07-02

文章信息/Info

Title:
Transformation of tobacco plants by Yali PPO-GFP fusion gene and observation of subcellular localization
作者:
齐 靖1* 李桂琴1 董 祯2 周 薇1
1. 河北经贸大学 生物科学与工程学院, 石家庄 050061; 2. 河北女子职业技术学院, 石家庄 050091
Author(s):
QI Jing1* LI Gui-Qin1 DONG Zhen2 ZHOU Wei1
1. College of Biology Science and Engineering, Hebei University of Economics and Business, Shijiazhuang 050061, China; 2. Hebei Women’s Vocational College, Shijiazhuang 050091, China
关键词:
鸭梨 多酚氧化酶 绿色荧光蛋白 基因融合 遗传转化 亚细胞定位
Keywords:
Yali polyphenol oxidase GFP gene fusion genetic transformation subcellular localization
分类号:
Q942.6
DOI:
-
文献标识码:
A
摘要:
将鸭梨PPO基因与绿色萤光蛋白GFP基因相融合共同进行遗传转化的方式,对鸭梨多酚氧化酶开展细胞定位研究。通过克隆该酶基因除终止密码子TAA外长度为1 779 bp的CDS序列,与绿色荧光蛋白基因重组构建了荧光表达载体pBI121-PPO-GFP,借助农杆菌转化烟草,转基因烟草叶片细胞经激光扫描共聚焦显微镜观察,绿色荧光蛋白荧光与叶绿体自发荧光相重合。结果表明鸭梨多酚氧化酶为叶绿体蛋白质。
Abstract:
To explore the subcellular localization of Polyphenol oxidase(PPO)from Pyrus bretschneideri,the 1 779 bp cDNA of PPO gene was cloned and fused with GFP in frame to construct a binary vector pBI121-PPO-GFP. Then,the binary vector was transformed into Nicotiana tabacum by the tumefanciens-mediated method. Using confocal laser scanning microscopy,green fluorescent signals were localized in chloroplasts of the transformed Nicotiana tabacum cell. This results suggested that the Polyphenol oxidase from Pyrus bretschneideri was a chloroplast protein.

参考文献/References

Abdel-Ghany SE,Müller-Moulé P,Niyogi KK,et al. 2005. Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts[J]. Plant Cell,17(4):1 233-1 251
Chevalier T,de Rigal D,Mbéguié-A-Mbéguié D,et al. 1999. Molecular cloning and characterization of apricot fruit polyphenol oxidase[J]. Plant Physiol,119:1 261-1 269
Dong Z(董祯). 2007. The study on the ACC oxidase genes clone of pear fruit(梨果实ACC氧化酶基因克隆的研究)[D]. Baoding(保定):Agricultural University of Hebei(河北农业大学)
Fraignier MP,Marquues L,Fleuriet A,et al. 1995. Biochemical and immunochemical characteristics of polyphenel oxidase from different fruits of Prunus[J]. J Agric & Food Chem,43:2 375-2 380
Gao H,Sage TL,Osteryoung KW. 2006. FZL,an FZO-like protein in plants,is a determinant of thylakoid and chloroplast morphology[J]. Proceed Nat Acad Sci USA,103(17):6 759-6 764
Haruta M,Murata M,Kadokura H,et al. 1999. Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees[J]. Phytochemistry,50(6):1 021-1 025
Horsch RB,Fry JE,Hoffman N. 1985. A simple and general method for transferring genes into plants[J]. Science,227:1 229-1 231
Hunt MD,Eannetta NT,Yu H,et al. 1993. cDNA cloning and expression of potato polyphenol oxidase[J]. Plant Mol Biol,21:59-68
Ju ZG(鞠志国),Song GL(朱广廉),Cao ZX(曹宗巽). 1998. The compartmentation of polyphenol oxidase and its substrates in relation with fruit browing of Laiyang Chili(Pyrus bretschneideri Rehd)(莱阳茌梨果实褐变与多酚氧化酶及酚类物质区域化分布的关系)[J]. Acta Phytophysiol Sin(植物生理学报),14(4):351-356
Ju ZG(鞠志国). 1989. Properties and distribution of polyphenol oxidase in cells of Pyrus bretschneideri(Laiyang Chili)(莱阳茌梨细胞中多酚氧化酶的分布及其性质)[J]. Plant Physiol Comm(植物生理学通讯),(3):33-35
Kim JY,Seo YS,Kim JE,et al. 2001. Two polyphenol oxidases are differentially expressed during vegetative and reproductive development and in response to wounding in the Fuji apple[J]. Plant Sci,161:1 145-1 152
Li GQ(李桂琴). 2009. Study on cloning and anti-transformation for polyphenol oxidase gene in ‘Yali’ Pear(鸭梨多酚氧化酶基因克隆及其反义转化的研究)[D]. Baoding(保定):Agricultural University of Hebei(河北农业大学) Pan D,Chen G,Lü L,et al. Isolation of full length Nai(Prunus salicina Lindl. )leaf polyphenol oxidase [DB/OL]. http://www. ncbi. nlm. nih. gov/entrez/viewer. fcgi?db=nuccore & id=58047495,2005-07-28/2008-01-20
Sherman TD,Vaughn KC,Duck SO. 1991. A limited survey of the phytogenetic distribution of polyphenol oxidase[J]. Phyto Chemisty,30:2 499-2 506
Smeekens S,Bauerle C,Hageman J,et al. 1986. The role of the transit peptide in the routing of precursors toward different chloroplast compartments[J]. Cell,46:365-375
Sommer A, Nèèman E, Steffens JC,et al. 1994. Import,targeting and processing of a plant polyphenol oxidase[J]. Plant Physiol,105:1 301-1 311
Thygesen PW,Dry IB,Robinson SP. 1995. Polyphenol oxidase in potato. A multigene family that exhibits differentional expression patterns[J]. Plant Physiol,109:525-531
Vaughn KC,Duke SO. 1984. Function of polyphenol oxidase in higher plants[J]. Plant Physiol,60:106-112
Wang M(汪敏),Zheng SZ(郑师章). 1994. Physiological and biochemical properties of the polyphenol oxidase in Eichhornia crassipes(凤眼莲体内多酚氧化酶的生理生化特性)[J]. J Fudan Univ:Nat Sci Edit(复旦学报·自然科学版),33(2):167-164
Wang GL(王关林),Fang HJ(方宏均). 2002. The Principle and Technology of Plant Gene Engineering(植物基因工程的原理与技术)[M]. Beijing(北京):Science Press(科学出版社)
Wang ML(王曼玲),Hu ZL(胡中立),Zhou MQ(周明全),et al. 2005. Advances in research of polyphenol oxidase in plants(植物多酚氧化酶的研究进展)[J]. Chin Bull Bot(植物学通报),22(2):215-222
Abdel-Ghany SE,Müller-Moulé P,Niyogi KK,et al. 2005. Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts[J]. Plant Cell,17(4):1 233-1 251
Chevalier T,de Rigal D,Mbéguié-A-Mbéguié D,et al. 1999. Molecular cloning and characterization of apricot fruit polyphenol oxidase[J]. Plant Physiol,119:1 261-1 269
Dong Z(董祯). 2007. The study on the ACC oxidase genes clone of pear fruit(梨果实ACC氧化酶基因克隆的研究)[D]. Baoding(保定):Agricultural University of Hebei(河北农业大学)
Fraignier MP,Marquues L,Fleuriet A,et al. 1995. Biochemical and immunochemical characteristics of polyphenel oxidase from different fruits of Prunus[J]. J Agric & Food Chem,43:2 375-2 380
Gao H,Sage TL,Osteryoung KW. 2006. FZL,an FZO-like protein in plants,is a determinant of thylakoid and chloroplast morphology[J]. Proceed Nat Acad Sci USA,103(17):6 759-6 764
Haruta M,Murata M,Kadokura H,et al. 1999. Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees[J]. Phytochemistry,50(6):1 021-1 025
Horsch RB,Fry JE,Hoffman N. 1985. A simple and general method for transferring genes into plants[J]. Science,227:1 229-1 231
Hunt MD,Eannetta NT,Yu H,et al. 1993. cDNA cloning and expression of potato polyphenol oxidase[J]. Plant Mol Biol,21:59-68
Ju ZG(鞠志国),Song GL(朱广廉),Cao ZX(曹宗巽). 1998. The compartmentation of polyphenol oxidase and its substrates in relation with fruit browing of Laiyang Chili(Pyrus bretschneideri Rehd)(莱阳茌梨果实褐变与多酚氧化酶及酚类物质区域化分布的关系)[J]. Acta Phytophysiol Sin(植物生理学报),14(4):351-356
Ju ZG(鞠志国). 1989. Properties and distribution of polyphenol oxidase in cells of Pyrus bretschneideri(Laiyang Chili)(莱阳茌梨细胞中多酚氧化酶的分布及其性质)[J]. Plant Physiol Comm(植物生理学通讯),(3):33-35
Kim JY,Seo YS,Kim JE,et al. 2001. Two polyphenol oxidases are differentially expressed during vegetative and reproductive development and in response to wounding in the Fuji apple[J]. Plant Sci,161:1 145-1 152
Li GQ(李桂琴). 2009. Study on cloning and anti-transformation for polyphenol oxidase gene in ‘Yali’ Pear(鸭梨多酚氧化酶基因克隆及其反义转化的研究)[D]. Baoding(保定):Agricultural University of Hebei(河北农业大学)
Pan D,Chen G,Lü L,et al. Isolation of full length Nai(Prunus salicina Lindl. )leaf polyphenol oxidase [DB/OL]. http://www. ncbi. nlm. nih. gov/entrez/viewer. fcgi?db=nuccore & id=58047495,2005-07-28/2008-01-20
Sherman TD,Vaughn KC,Duck SO. 1991. A limited survey of the phytogenetic distribution of polyphenol oxidase[J]. Phyto Chemisty,30:2 499-2 506
Smeekens S,Bauerle C,Hageman J,et al. 1986. The role of the transit peptide in the routing of precursors toward different chloroplast compartments[J]. Cell,46:365-375
Sommer A, Nèèman E, Steffens JC,et al. 1994. Import,targeting and processing of a plant polyphenol oxidase[J]. Plant Physiol,105:1 301-1 311
Thygesen PW,Dry IB,Robinson SP. 1995. Polyphenol oxidase in potato. A multigene family that exhibits differentional expression patterns[J]. Plant Physiol,109:525-531
Vaughn KC,Duke SO. 1984. Function of polyphenol oxidase in higher plants[J]. Plant Physiol,60:106-112
Wang M(汪敏),Zheng SZ(郑师章). 1994. Physiological and biochemical properties of the polyphenol oxidase in Eichhornia crassipes(凤眼莲体内多酚氧化酶的生理生化特性)[J]. J Fudan Univ:Nat Sci Edit(复旦学报·自然科学版),33(2):167-164
Wang GL(王关林),Fang HJ(方宏均). 2002. The Principle and Technology of Plant Gene Engineering(植物基因工程的原理与技术)[M]. Beijing(北京):Science Press(科学出版社)
Wang ML(王曼玲),Hu ZL(胡中立),Zhou MQ(周明全),et al. 2005. Advances in research of polyphenol oxidase in plants(植物多酚氧化酶的研究进展)[J]. Chin Bull Bot(植物学通报),22(2):215-222

备注/Memo

备注/Memo:
收稿日期: 2013-10-10修回日期: 2013-12-22
基金项目: 河北省科技计划项目(11220103D-9); 河北省高等学校科学技术研究指导项目(Z2012065); 河北省高等学校科学研究计划重点项目(ZH2011126)。
作者简介: 齐靖(1981-),男,河北石家庄人,博士,讲师,主要从事植物生理与分子生物学方面研究,(Email)qijing@heuet.edu.cn。
更新日期/Last Update: 2014-05-25