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拉雅松遗传多样性及同域分布种间亲缘关系分析(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2014年03期
页码:
320-325
栏目:
系统演化与生物多样性
出版日期:
2014-07-02

文章信息/Info

Title:
Genetic diversity of Pinus crassicorticea and its genetic relationship with two sympatric pine species
作者:
冯源恒12 吴东山3 王德俊1 杨章旗2 李火根1*
1. 南京林业大学 林木遗传与生物技术省部共建教育部重点实验室, 南京 210037; 2. 广西 壮族自治区林业科学研究院, 南宁 530002; 3. 广西大学, 南宁 530001
Author(s):
FENG Yuan-Heng12 WU Dong-Shan3 WANG De-Jun1 YANG Zhang-Qi2 LI Huo-Gen1*
1. Key Lab of Forest Genetics and Biotechnology(Nanjing Forestry University), Ministry of Education, Nanjing 210037, China; 2. Guangxi Institute of Forestry Science, Nanning 530002, China; 3. Guangxi University, Nanning 530001, China
关键词:
拉雅松 SSR 遗传多样性 同域分布 亲缘关系
Keywords:
Pinus crassicorticea SSR genetic diversity sympatric genetic relationship
分类号:
Q347; S718.46
DOI:
-
文献标识码:
A
摘要:
拉雅松是广西西北部稀有的乡土用材、用脂树种,有较高的经济价值,但其遗传多样性状况及种间进化关系未知。利用SSR分子标记检测拉雅松群体遗传多样性,希望对该物种保护策略的制定提供参考依据。此外,鉴于该地区自然分布的松属树种仅有拉雅松、细叶云南松与马尾松三个种,试图利用SSR分子标记信息分析拉雅松与细叶云南松、马尾松的种间亲缘关系。结果表明:7对SSR引物在拉雅松群体共检测到14个等位基因。有效等位基因数为1.653,观测杂合度为0.577,期望杂合度为0.374,Shannon信息指数为0.540,Nei多样性指数为0.367,表明拉雅松具有较高的遗传多样性。拉雅松与马尾松遗传距离最近为0.0175,与3个细叶云南松群体距离较远,平均为0.0525。拉雅松与马尾松、细叶云南松平均共祖系数(Θ)分别为0.094、0.066,据此推测拉雅松可能与马尾松存在较近的亲缘关系。讨论了拉雅松的遗传多样性保护策略。
Abstract:
Pinus crassicorticea is a rare tree species has high economical value in northwestern Guangxi. As the habitat has been seriously damaged,P. crassicorticea is now regarded as an endangered species. To preserve the germplasm of this species,the genetic diversity of P. crassicorticea was quantified based on 7 SSR loci. The interspecies relationship between P. crassicorticea and two sympatric pine species,P. yunnanensis var. tenuifolia and P. massoniana,was further analyzed. The results showed that the mean number of effective alleles(Ne)per locus was 1.653,the average observed heterozygosity(Ho)was 0.577,the expected heterozygosity(He)was 0.374,Shannon’s information index(I)was 0.540 and mean Nei’s gene diversity(h)was 0.376,indicating that P. crassicorticea maintains high levels of genetic variation. The genetic identity(GD)was 0.0175 between P. crassicorticea and P. massoniana,while 0.0525 between P. crassicorticea and P. yunnanensis var. tenuifolia. The interspecies relationship coefficient was 0.094 within P. crassicorticea and P. massoniana,and 0.066 in P. crassicorticea and P. yunnanensis var. tenuifolia, respectively. Therefore,it was reasonable that P. crassicorticea was genetically closer to P. massoniana than P. yunnanensis var. tenuifoli. The strategy for the genetic resource conservation of P. crassicorticea was also discussed in this paper.

参考文献/References

Bennuah SY,Wang T,Aitken SN. 2004. Genetic analysis of the Picea stichensis�/i>215;glauca introgression zone in British Columbia[J]. For Ecol Manage,197:65-77
Chen FY(陈凤英),Zeng KW(曾科文),Xiao XH(肖向红),et al. 1997. Consanguineous relationship of three species of Sect. cembre spach of Pinus from Daxing. am Mountiansin China-a comparative study on the POD isonzyme zymogram(大兴安岭三类五针松亲缘关系-过氧化物酶同工酶谱比较)[J]. J Northeast For Univ(东北林业大学学报),25(6):48-51
Chen SY,Zhao WS,Wang J. 2002. Genetic diversity and genetic differentiation of natural populations of Pinus kesiya var. langbinanensis[J]. J For Res,13(4):273-276
Cheng JF(陈金凤). 1993. Deter Mination of the size of wood fibre(Tracheid)from 10 species of Pinus in Guangxi(广西松属10种木材纤维(管胞)的测定)[J]. J Guangxi Agric Univ(广西农业大学学报),12(1):78-81
Dow BD,Ashley MV. 1996. Microsatellite analysis of seed dispersal and parentage of saplings in Bur oak,Quercus macrocarpa[J]. Theor Appl Genet,91(1):137-141
Dow B D. Ashley MV. 1998. High levels of gene flow in Bur oak revealed by paternity analysis using microsatellites[J]. J Hered,89(1):62-70
Doyle JJ,Doyle JL. 1990. Isolation of plant DNA from fresh tissue[J]. Focus,12:13-15
Elston RC. 1971. The estimation of admixture in racial hybrids[J]. Annal Human Gen,35:9-17
Fu XX(洑香香),Zhao H(赵虎),Wang Y(王玉). 2011. Species identification and genetic relationship assessment of Pinus(sect. Pinus)related species based on morphological and molecular markers(松属近缘种形态和分子鉴定及其亲缘关系探讨)[J]. Sci Silv Sin(林业科学),47(10):51-58
Gizendanner MA,Soltis PS. 2000. Patterns of genetic variation in rKre and widespread plant congenem[J]. Am J Bot,87:783-792
Hamrick JL,Loveless MD. 1992. Factors influencing levels of genetic diversity in woody plant species[J]. New For,6:95-124
Labbé J,Zhang XY,Yin TM,et al. 2008. A genetic linkage map for the ectom ycorrhizal fungus Laccaria bicolor and its alignment to the whole-genome sequence assemblies[J]. New Phytol,180:316-328
Li M(李明),Wang SX(王树香),Gao BJ(高宝嘉). 2013. Analysis of genetic diversity of chinese pine(Pinus tabulaeformis)natural secondary forest populations and correlation with theirshabitat ecological factors(油松天然次生林居群遗传多样性及与产地地理气候因子的关联分析)[J]. Acta Ecol Sin(生态学报),33(12):3 602-3 610
Li Z(李梓). 2012. Extraction technology and bioactivity research of volatile components from Pinus crassicorticea and Pinus yunnanensis Franch. var. tenuifolia(拉雅松和细叶云南松挥发性成分的提取及生物活性研究)[D]. Liuzhou(广西柳州): Guangxi University Technology(广西工学院)
Mou JP(牟继平). 1993. Study on the wood structure of Pinus crassicorticea(拉雅松木材构造的研究)[J]. J Guangxi Agric Univ(广西农业大学学报),12(2):56-59
Rasmussen KK,Kollmann J. 2008. Low genetic diversity in small peripheral populations of a rare European tree(Sorbus torminalis)dominated by clonal reproduction[J]. Conser Gen,9:1 533-1 539
Stoeckel S,Grange J,Fernandez-Manjarres JF,et al. 2006. Heterozygote excess in a self-incompatible and partially clonal forest tree species-Prunus avium L[J]. Molec Ecol,15:2 109-2 118
Tan XM(谭小梅),Zhou ZC(周志春),Jin GQ(金国庆),et al. 2012. Genetic diversity and mating system analysis of Pinus massoniana in a second-generation clonal seed orchard(马尾松二代无性系种子园遗传多样性和交配系统分析)[J]. Sci Silv Sin(林业科学),48(2):69-74
Tang JJ(唐娟娟),Fan YR(范义荣),Zhu HY(朱睦元). 2003. Analysis of the genetic diversity of Pinus taiwanensis populations(黄山松群体遗传多样性分析)[J]. J Zhejiang For Coll(浙江林学院学报),20(1):23-26
Wang JL. 2011. Coancestry:a program for simulating,estimating and analysing relatedness and inbreeding coefficients[J]. Molec Ecol Resour,11:141-145
Wang X(王霞),Wang J(王静),Jiang JH(蒋敬虎),et al. 2012. Genetic diversity and the mating system in a fragmented population of Tsoongiodendron odorum(观光木片断化居群的遗传多样性和交配系统)[J]. Biodiver Sci(生物多样性),20(6):676-684
Wei XL(魏小玲),Cao FX(曹福祥),Chen J(陈建). 2013. Studies on the genetic diversity and relationship of Manglietia hainanensis Dandy by ISSR(海南木莲遗传多样性的ISSR 及亲缘关系的分析)[J]. Biotechnol Bull(生物技术通报). 8:74-77
Wyman J,Bruneau A,Tremblay MF. 2003. Microsatellite analysis of genetic diversity in four populations of Populus tremuloides in Quebec[J]. Can J Bot,81:360-367
Yeh FC,Yang RC,Boyle TB,et al. 1997. POPGENE,the user-friendly shareware for population genetic analysis[J]. Molec Biol Biotechnol Centre,Univ Alberta,Canada,10
Yu H(虞泓),Ge S(葛颂),Huang RF(黄瑞复),et al. 2000. A Preliminary study on genetic variation and relationships of Pinus yunnanensis and its closely related species(云南松及其近缘种的遗传变异与亲缘关系)[J]. Acta Bot Sin(植物学报),42(1):107-110
Zhang HL(张红莲),Li HG(李火根),Xu M(胥猛),et al. 2010. Identification of Liriodendron tulipifera,Liriodendron chinense and hybrid Liriodendron using species-specific SSR markers[J]. Sci Silv Sin(林业科学),46(1):36-39
Zhong YC(钟业聪),Huang KX(黄开响). 1990. A new species of Pinus in Guangxi(广西松属一新种)[J]. Guihaia(广西植物),10(4):287

备注/Memo

备注/Memo:
收稿日期: 2013-10-26修回日期: 2013-12-11
基金项目: 广西八桂学者专项经费; 江苏省研究生创新基金(CXZZ12_0541)。
作者简介: 冯源恒(1981-),男,黑龙江牡丹江市人,博士研究生,工程师,主要从事林木遗传育种研究,(E-mail)nanyuan05@163.com。 *通讯作者: 李火根,博士,教授,主要从事林木遗传育种研究,(E-mail)hgli@njfu.edu.cn。
更新日期/Last Update: 2014-05-25