我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|本期目录/Table of Contents|

受非生物胁迫和稻瘟病胁迫双重诱导的OsWRKY转录因子基因表达特征分析(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2014年02期
页码:
248-255
栏目:
学科栏目
出版日期:
2014-05-15

文章信息/Info

Title:
Gene expression analysis of five OsWRKY transcription factors induced by abiotic and rice blast stresses
作者:
徐 展 林良斌
云南农业大学 农学与生物技术学院, 昆明 650201
Author(s):
XU Zhan LIN Liang-Bin*
College of Agriculture and Biotechnology, Yunnan Agriculture University, Kunming 650201, China
关键词:
OsWRKY 转录因子 稻瘟病胁迫 非生物胁迫 实时定量PCR 基因表达
Keywords:
OsWRKY transcription factor rice blast stress abiotic stress real-time PCR gene expression
分类号:
Q945.78
DOI:
10.3969/j.issn.1000-3142.2014.02.021
文献标识码:
A
摘要:
OsWRKY转录因子在水稻非生物胁迫和抗病反应中具有相当重要的调节作用。为阐明其调节作用提供依据,研究了疑似功能广泛的OsWRKY转录因子表达谱,采用五个OsWRKY转录因子基因,即OsWRKY7、OsWRKY11、OsWRKY30、OsWRKY70和OsWRKY89,利用real-time PCR研究各种非生物胁迫和稻瘟菌胁迫诱导表达特征,以及各种激素对OsWRKY表达量的影响。所采用的五个基因均受到稻瘟菌胁迫的诱导,而且各种非生物胁迫也能不同程度地诱导其表达。在各个激素处理下,有些被诱导或被抑制,也有未受影响。五个OsWRKY基因均有可能参与稻瘟病胁迫响应。其中OsWRKY7和OsWRKY70可能是在JA和SA相互拮抗调控下参与,OsWRKY89可能是通过非本研究涉及的其他激素途径参与。在非生物胁迫方面,OsWRKY7可能通过ABA途径参与干旱、高盐和极端温度胁迫; OsWRKY11有可能参与高盐胁迫; OsWRKY30有可能参与高盐和高温胁迫; OsWRKY70可能参与高盐、干旱和极端温度胁迫; OsWRKY89可能参与高温胁迫,但并不是通过本研究所涉及的四种激素途径。
Abstract:
OsWRKY transcription factors have vital function in respond to adverse environment and pathogens in rice. In order to elucidate the regulatory role of these genes,we used Realtime-PCR to investigate expression profiles of five OsWRKY transcription factors(OsWRKY7,OsWRKY11,OsWRKY30,OsWRKY70 and OsWRKY89)under abiotic stresses and pathogen challenges. The expression characteristics of hormone treatments were also investigated in order to study which hormone way they were functioning through. All five OsWRKY could be induced by pathogen inoculation and all these genes had different rate inductions under abiotic stress. In each hormone treatment,some were induced,others were suppressed,and still others unaffected. Five OsWRKY genes were all likely to be involved in the infection process of rice blast stress. OsWRKY7 and OsWRKY70 might participate in the resistance to rice blast by JA and SA in a mutually antagonistical manner; OsWRKY89 also might be involved in it,but not through the four hormone ways studied in this research. In the aspect of abiotic stress,OsWRKY7 might participate in drought,high salt and extreme temperature stresses through the ABA way; OsWRKY11 had the possibility to be involved in high salt stress; OsWRKY30 was possible to participate in high salt and hot stresses; OsWRKY70 might be involved in high salt,drought and extreme temperature stresses; OsWRKY89 might be involved in hot stress,but not through the four hormone ways studied in this research.OsWRKY transcription factors have vital function in respond to adverse environment and pathogens in rice. In order to elucidate the regulatory role of these genes,we used Realtime-PCR to investigate expression profiles of five OsWRKY transcription factors(OsWRKY7,OsWRKY11,OsWRKY30,OsWRKY70 and OsWRKY89)under abiotic stresses and pathogen challenges. The expression characteristics of hormone treatments were also investigated in order to study which hormone way they were functioning through. All five OsWRKY could be induced by pathogen inoculation and all these genes had different rate inductions under abiotic stress. In each hormone treatment,some were induced,others were suppressed,and still others unaffected. Five OsWRKY genes were all likely to be involved in the infection process of rice blast stress. OsWRKY7 and OsWRKY70 might participate in the resistance to rice blast by JA and SA in a mutually antagonistical manner; OsWRKY89 also might be involved in it,but not through the four hormone ways studied in this research. In the aspect of abiotic stress,OsWRKY7 might participate in drought,high salt and extreme temperature stresses through the ABA way; OsWRKY11 had the possibility to be involved in high salt stress; OsWRKY30 was possible to participate in high salt and hot stresses; OsWRKY70 might be involved in high salt,drought and extreme temperature stresses; OsWRKY89 might be involved in hot stress,but not through the four hormone ways studied in this research.

参考文献/References

Bai XQ,Liu XQ,Qian Q,et al. 2005. OsWRKY03,a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1[J]. Cell Res,15(8): 593-603
Bai XQ,Liu XQ,Wang XJ,et al. 2007. OsWRKY71,a rice transcription factor,is involved in rice defense response[J]. J Plant Physiol,164(8): 969-979
Barth M,Kalde M,Somssich IE,et al. 2003. Members of the Arabidopsis WRKY group III transcription factors are part of different plant defense signaling pathways[J]. Mol Plant-Microb Inter,16:295-305
Brader G,Li J,Palva ET. 2004. The WRKY70 transcription factor:a node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense[J]. Plant Cell,16(2):319-331
Chen CH,ChenZX,Yu DQ. 2001. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression[J]. Plant Cell,13(7):1 527-1 540
Chen XJ,Hao JJ,Wang HH,et al. 2007. Overexpression of rice WRKY89 enhances ultraviolet B tolerance and disease resistance in rice plants[J]. Plant Mol Bio,65:799-815
Chomczynski P,Sacchi N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction[J]. Analy Biochem,162(1):156-159
Ding XH,Qiu DY,Xiao J,et al. 2007. OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate-and jasmonate dependent signaling[J]. Mol Plant-Microb Inter,20(5):492-499
Ebneth M,Hildmann T,Pe?a-Cortés H,et al. 1992. General roles of abscisic and jasmonic acids in gene activation as a result of mechanical wounding[J]. Plant Cell,4:1 157-1 170
Eulgem T,Rushton PJ,Robatzek S,et al. 2000. The WRKY superfamily of plant transcription factors[J]. Trend Plant Sci,5(5):199-206
Eulgem T,Somssich IE. 2007. Networks of WRKY transcription factors in defense signaling[J]. Curr Opin Plant Biol,10:366-371
Guo ZJ,Peng YL,Zhang J. 2008. Constitutive expression of pathogen inducible OsWRKY31 enhances disease resistance and affects root growth and auxin response in transgenic rice plants[J]. Cell Res,18:508-521
Guo ZJ,Wang HH,Wu KL,et al. 2005. The WRKY family of transcription factors in rice and Arabidopsis and their origins[J]. DNA Res,12(1):9-26
Hammond-Kosack KE,Parker JE. 2003. Deciphering plant pathogencommunication:fresh perspectives for molecular resistance breeding[J]. Curr Opin Plant Biol,14:177-193
Hu Y,Peng X,Tang X,et al. 2012 Nov. Constitutive expression of rice WRKY30 gene increases the endogenous jasmonic acid accumulation,PR gene expression and resistance to fungal pathogens in rice[J]. Planta,236(5):1 485-98
Hu YJ(胡耀军),Peng XX(彭喜旭),Tang XK(唐新科),et al. 2011. Isolation and expression profiles of rice WRKY30 induced by jasmonic acid application and fungal pathogen infection(受JA和真菌病原接种诱导的OsWRKY30表达谱)[J]. Sci Agric Sin(中国农业科学),44(12):2 454-2 461
Jena KK. 2006. Beating blast[J]. Rice Today,1:36-37
Jiang SY,Ramamoorthy R,et al. 2008. A comprehensive transcriptional profiling of the WRKY gene family in rice under various abiotic and phytohormone treatments[J]. Plant Cell Physiol,49(6):865-879
Jin J,LeeYW,Park JY,et al. 2009. Rice blast fungus(Magnaporthe oryzae)infects Arabidopsis via a mechanism distinct from that required for the infection of rice[J]. Plant Physiol,149:474-486
Jing SJ,Song Y,Zhou X,et al. 2009. Heterologous expression of OsWRKY23 gene enhances pathogen defense and dark-induced leaf senescence in Arabidopsis[J]. Plant Growth Reg,58:181-190
Johnson CS,Kolevski B,et al. 2002. Transparent testa glabra 2,a trichome and seed coat development gene of Arabidopsis,encodes a WRKY transcription factor[J]. Plant Cell,14(6):1 359-1 375
Kishitani S,Shiroto Y,Wu X,et al. 2009. Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter[J]. Plant Cell Rep,28(1):21-30
LiuC,Shen H,Zhang Y,et al. 2012. OsWRKY30 is activated by MAP kinases to confer drought tolerance in rice[J]. Plant Mol Biol,80(3):241-53
Pandey SP,Somssich IE. 2009. The role of WRKY transcription factors in plant immunity[J]. Plant Physiol,150:1 648-1 655
Peng YL,Shishiyama J. 1988. Temporal sequence of cytological events in rice leaves affected with Pyricularia oryzae[J]. Can J Bot,66:730-735
Qiu YP,Yu DQ. 2009. Over-expression of the stress-induced OsWRKY45 enhances disease resistance and drought tolerance in Arabidopsis[J]. Environ Exper Bot,65:35-47
Somssich IE,?lker B. 2004. WRKY transcription factors:from DNA binding towards biological function[J]. Curr Opin Plant Biol,7:491-498
Bai XQ,Liu XQ,Qian Q,et al. 2005. OsWRKY03,a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1[J]. Cell Res,15(8): 593-603
Bai XQ,Liu XQ,Wang XJ,et al. 2007. OsWRKY71,a rice transcription factor,is involved in rice defense response[J]. J Plant Physiol,164(8): 969-979
Barth M,Kalde M,Somssich IE,et al. 2003. Members of the Arabidopsis WRKY group III transcription factors are part of different plant defense signaling pathways[J]. Mol Plant-Microb Inter,16:295-305
Brader G,Li J,Palva ET. 2004. The WRKY70 transcription factor:a node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense[J]. Plant Cell,16(2):319-331
Chen CH,ChenZX,Yu DQ. 2001. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression[J]. Plant Cell,13(7):1 527-1 540
Chen XJ,Hao JJ,Wang HH,et al. 2007. Overexpression of rice WRKY89 enhances ultraviolet B tolerance and disease resistance in rice plants[J]. Plant Mol Bio,65:799-815
Chomczynski P,Sacchi N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction[J]. Analy Biochem,162(1):156-159
Ding XH,Qiu DY,Xiao J,et al. 2007. OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate-and jasmonate dependent signaling[J]. Mol Plant-Microb Inter,20(5):492-499
Ebneth M,Hildmann T,Pe?a-Cortés H,et al. 1992. General roles of abscisic and jasmonic acids in gene activation as a result of mechanical wounding[J]. Plant Cell,4:1 157-1 170
Eulgem T,Rushton PJ,Robatzek S,et al. 2000. The WRKY superfamily of plant transcription factors[J]. Trend Plant Sci,5(5):199-206
Eulgem T,Somssich IE. 2007. Networks of WRKY transcription factors in defense signaling[J]. Curr Opin Plant Biol,10:366-371
Guo ZJ,Peng YL,Zhang J. 2008. Constitutive expression of pathogen inducible OsWRKY31 enhances disease resistance and affects root growth and auxin response in transgenic rice plants[J]. Cell Res,18:508-521
Guo ZJ,Wang HH,Wu KL,et al. 2005. The WRKY family of transcription factors in rice and Arabidopsis and their origins[J]. DNA Res,12(1):9-26
Hammond-Kosack KE,Parker JE. 2003. Deciphering plant pathogencommunication:fresh perspectives for molecular resistance breeding[J]. Curr Opin Plant Biol,14:177-193
Hu Y,Peng X,Tang X,et al. 2012 Nov. Constitutive expression of rice WRKY30 gene increases the endogenous jasmonic acid accumulation,PR gene expression and resistance to fungal pathogens in rice[J]. Planta,236(5):1 485-98
Hu YJ(胡耀军),Peng XX(彭喜旭),Tang XK(唐新科),et al. 2011. Isolation and expression profiles of rice WRKY30 induced by jasmonic acid application and fungal pathogen infection(受JA和真菌病原接种诱导的OsWRKY30表达谱)[J]. Sci Agric Sin(中国农业科学),44(12):2 454-2 461
Jena KK. 2006. Beating blast[J]. Rice Today,1:36-37
Jiang SY,Ramamoorthy R,et al. 2008. A comprehensive transcriptional profiling of the WRKY gene family in rice under various abiotic and phytohormone treatments[J]. Plant Cell Physiol,49(6):865-879
Jin J,LeeYW,Park JY,et al. 2009. Rice blast fungus(Magnaporthe oryzae)infects Arabidopsis via a mechanism distinct from that required for the infection of rice[J]. Plant Physiol,149:474-486
Jing SJ,Song Y,Zhou X,et al. 2009. Heterologous expression of OsWRKY23 gene enhances pathogen defense and dark-induced leaf senescence in Arabidopsis[J]. Plant Growth Reg,58:181-190
Johnson CS,Kolevski B,et al. 2002. Transparent testa glabra 2,a trichome and seed coat development gene of Arabidopsis,encodes a WRKY transcription factor[J]. Plant Cell,14(6):1 359-1 375
Kishitani S,Shiroto Y,Wu X,et al. 2009. Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter[J]. Plant Cell Rep,28(1):21-30
LiuC,Shen H,Zhang Y,et al. 2012. OsWRKY30 is activated by MAP kinases to confer drought tolerance in rice[J]. Plant Mol Biol,80(3):241-53
Pandey SP,Somssich IE. 2009. The role of WRKY transcription factors in plant immunity[J]. Plant Physiol,150:1 648-1 655
Peng YL,Shishiyama J. 1988. Temporal sequence of cytological events in rice leaves affected with Pyricularia oryzae[J]. Can J Bot,66:730-735
Qiu YP,Yu DQ. 2009. Over-expression of the stress-induced OsWRKY45 enhances disease resistance and drought tolerance in Arabidopsis[J]. Environ Exper Bot,65:35-47
Somssich IE,?lker B. 2004. WRKY transcription factors:from DNA binding towards biological function[J]. Curr Opin Plant Biol,7:491-498

备注/Memo

备注/Memo:

收稿日期: 2013-08-23修回日期: 2013-10-25
基金项目: 中国科学院转基因生物新品种培育科技重大专项(2009ZX08009-066B)
作者简介: 徐展(1986-),男,浙江浦江人,硕士,主要从事植物分子生物学研究,(E-mail)gxuxuzhan@sina.com。
*通讯作者: 林良斌,博士,教授,主要从事植物分子生物学研究,(E-mail)binliangbin@163.com。
更新日期/Last Update: 2014-04-10