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基于FTIR的三倍体罗汉果花粉直感效应研究(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2014年01期
页码:
95
栏目:
植物生理与分子生物学
出版日期:
2014-01-25

文章信息/Info

Title:
Xenia effect of triploid Siraitia grosvenorii based on fourier transform infrared spectroscopy
作者:
李林轩 闫海锋 蒋水元 孔德鑫 黄夕洋
1. 广西药用植物园/广西药用资源保护与遗传改良重点实验室, 南宁 530023; 2. 广西农业科学院 经济作物研究所, 南宁 530007; 3. 广西壮族自治区中国科学院 广西植物研究所, 广西 桂林 541600
Author(s):
LI Lin-Xuan1 YAN Hai-Feng2 JIANG Shui-Yuan3 KONG De-Xin3 HUANG Xi-Yang3*
1. Guangxi Botanical Garden of Medicinal Plant/Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Nanning 530023, China; 2. Cash Crop Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 3. Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China
关键词:
傅里叶变换红外光谱 三倍体罗汉果 果实 花粉直感效应
Keywords:
fourier transform infrared spectroscopy triploid Siraitia grosvenorii fruit xenia effect
分类号:
Q945.7
DOI:
10.3969/j.issn.1000-3142.2014.01.018
文献标识码:
-
摘要:
以四个品系的三倍体罗汉果雌株为材料,用五种不同的二倍体雄花分别对其授粉,利用傅里叶变换红外光谱法(FTIR)测定了其子代果实的红外光谱,并运用主成分分析和聚类分析研究了授粉雄花对子代果实化学成分的影响。结果表明:不同雄花授粉后,雌株F302、F323和F322各子代果实红外光谱中1 050 cm-1波数附近甜苷物质特征吸收峰的峰高有显著或极显著差异,雌株F311子代无籽果实的差异不显著; 同时五种授粉雄花分别对雌株F302、F323、F322和F311子代果实在主成分二维投影图和聚类图中的排序也有明显的影响,但对不同品系雌株子代果实的排序影响不同,从而说明三倍体罗汉果的甜苷物质含量和整体成分含量均有较为明显的花粉直感效应,并且存在品种特异性。
Abstract:
The triploid female Siraitia grosvenorii of four strains were pollinated with five different diploid male flowers of S. grosvenorii,respectively. The infrared spectrums of the fruits of their offspring were measured by fourier transform infrared spectroscopy(FTIR),and the effects of male flowers on chemical component of offspring fruits were studied using principal component analysis(PCA)and clustering analysis. The results showed that there was significantly diffirent between peak height of sweet glycosides material characteristic absorption peak in infrared spectroscopy 1 050 cm-1(wave number)among females(F302, F323 and F322)offspring fruits,which was not significantly diffirent in female F311,while five kinds of female plants pollinated male F302, F323, F322 and F311 progeny fruit in two-dimensional projection of the main component and cluster diagram sort also had a significant impact,but for different strains of fruit sorting female plants affected offspring different,thus described triploid Mangosteen sweet glycosides ingredient content material content, and overall had a more significant effect Xenia, and there is variety-specific.

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备注/Memo

备注/Memo:
收稿日期: 2013-07-12 修回日期: 2013-09-04
基金项目: 国家自然科学基金(40961019); 广西科学研究与技术开发计划项目(桂科攻11107010-2-10,桂科重1355001-2-6); 广西自然科学基金(2011GXNSFC018022); 国家“十二五”科技支撑计划项目(2011BAI01B03)。
作者简介: 李林轩(1986-),男,广西全州人,助理研究员,从事药用植物资源保护与开发利用研究,(E-mail)starry1125@sina.com。
*通讯作者: 黄夕洋,副研究员,主要从事药用植物学和分子生物学研究,(E-mail)xiyang0687@163.com。
更新日期/Last Update: 2014-03-10