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用AFLP分析广东省鲜食橄榄品种资源遗传多样性(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2014年01期
页码:
4
栏目:
系统演化与生物多样性
出版日期:
2014-01-25

文章信息/Info

Title:
Genetic diversity analysis with AFLP on fresh Chinese olive cultivars resources in Guangdong Province
作者:
周俊辉 王燕平 陈 勤 周志钦
1. 仲恺农业工程学院 园艺园林学院, 广州 510225; 2. 广东省潮洲市茶叶科学研究中心, 广东 潮州 515726; 3. 西南大学 园艺园林学院, 重庆 400716
Author(s):
ZHOU Jun-Hui1* WANG Yan-Ping13 CHEN Qin2 ZHOU Zhi-Qin3
1. College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; 2. Chaozhou Institute Center of Tea, Chaozhou 515726, China; 3. College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China
关键词:
AFLP 橄榄 遗传多样性 亲缘关系
Keywords:
AFLP fresh Chinese olive genetic diversity
分类号:
Q949
DOI:
10.3969/j.issn.1000-3142.2014.01.002
文献标识码:
-
摘要:
从64对引物组合中筛选出6对谱带清晰、多态性高的引物组合,对63个橄榄品种扩增,共扩增出417条多态性电泳谱带,多态性比率达100%,揭示了橄榄品种的遗传多样性。利用UPGMA对供试橄榄品种聚类,建立AFLP聚类图。结果表明:63份材料之间的遗传相似性系数为0.0606~0.7619,相似系数为0.312时,可将供试材料分为7个品种群,其中第1群包括45个品种,以‘潮阳甜榄’为代表,还可细分成3个组; 第2群包括5个品种,以‘大纳甜’为代表; 第3群包括4个品种,以‘潮阳三棱’为代表; 第4群包括‘土甜’、‘文祠三棱’和‘早花三棱’3个品种; 第5群包括‘铁条’和‘细粒’两个品种; 第6群包括‘广太甜种’、‘西土’、‘鸡心’和‘车酸1号’4个品种; 第7群只有‘凤湖橄榄’1个品种,与其他品种群亲缘关系较远,可能发生变异。
Abstract:
Screened from 64 pairs of the AFLP primer,six pairs(E/AAC-M/CAT,E/AAC-M/CTT,E/ACA-M/CAA,E/AGG-M/CAA,E/ACC-M/CAA,E/ACT-M/CAT),which produced clear and reliable polymorphic bands,were selected for analysis genetic relationship and genetic diversity among the 63 Chinese olive cultivars. 417 amplification bands were produced with 100% polymorphic rate. It was indicated that there was rich genetic diversity in Chinese olive cultivar germplasm. The genetic relationship of the 63 Chinese olive cultivars was analyzed with software NTSYSpc-2.10a,and a UPGMA tree was established. According to this tree,the Chinese olive cultivars in this studied could be divided into 7 cultivar groups with a similar coefficient 0.312,the Group 1 delegated by ‘Chaoyang sweet olive’ including 45 cultivars,could be divided into 3 types,the Group 2 delegated by ‘Danatian’ including 5 cultivars,the Group 3 delegated by ‘Chaoyang sanleng’ including 4 cultivars,the Group 4 including 3 cultivars,the Group 5 including 2 cultivars,the Group 6 including 4 cultivars,and the Group 7 including one cultivar only,that was ‘Fenghu olive’,which had a long distance in relationship with other cultivars,maybe occurred variation in cultivation practice.

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备注/Memo

备注/Memo:
收稿日期: 2013-08-16修回日期: 2013-11-25
基金项目: 广东省科技计划项目(2010B020305012); 广东省农业科技项目(200844512)。
作者简介: 周俊辉(1963-),男,江西临川人,博士,教授,主要从事园艺植物生理学研究,(E-mail)junhuizhou@163.com。
更新日期/Last Update: 2014-03-10