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|本期目录/Table of Contents|

秋水仙素对蚕豆胚根生长的影响及多倍体诱导效应分析

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2012年03期
页码:
386-391
栏目:
植物生理与分子生物学
出版日期:
2012-07-05

文章信息/Info

Title:
Influence of colchicine on radicle growth of Vicia faba and the polyploid inductive effect
作者:
闫秋洁 杨 琼
绵阳师范学院 生命科学与技术学院, 四川 绵阳 621000
Author(s):
YAN QiuJie YANG Qiong
College of Life Sciences and Technology, Mianyang Normal University, Mianyang 621000, China
关键词:
多倍体 蚕豆 秋水仙素 胚根
Keywords:
polyploid Vicia faba colchicine radicle
分类号:
-
DOI:
-
文献标识码:
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摘要:
筛选秋水仙素诱导蚕豆胚根多倍体的最适诱导处理组合并分析其诱导效应。以秋水仙素5个浓度(0.025%、0.050%、0.100%、0.150%、0.200%)和4个诱导时间(12、24、48、60 h)正交组合(蒸馏水处理为对照)分别处理蚕豆胚根。以根尖诱导率和胚根膨大率的显著性差异确定蚕豆胚根多倍体的最适诱导处理组合,并以胚根细胞染色体数目和幼叶气孔数目的变化对诱导效果进行鉴定,分析其幼苗期的胚根数目和幼苗长度。结果表明:秋水仙素最适诱导时间为48 h和浓度为0.100%;气孔数量在诱导时间和诱导浓度之间均有显著差异,但幼苗期的胚根数量和幼苗长度在不同的诱导时间之间无显著差异,诱导浓度之间差异显著。
Abstract:
In order to test the optimum induction combination(time and concentration),and analyse the inductive effects of colchicine induced the radicle polyploid in Vicia faba. The radicle cells were treated with the colchicine of 〖JP3〗five concentrations (0.025%,0.050%,0.100%,0.150%,0.200%,respectively) combining various culture time (12,24,48,60 h,respectively). And the radicle cell were treated with distilled water simultaneously,which were taken as the control. The 〖JP2〗optimum induction combination induced by colchicine was confirmed by significant difference of the root tip inductivity and the radicle expanding ratio. And the chromosome number of radicle cells and stoma number of seedling were detected to identify the induction effect. The radicle amount and seeding height were analized subsequently. The optimum inductive condition was 48 h combining colchicine concentration of 0.100%. There were significant differences in stoma number between inducted time and inducted concentration,however,the radicle number and height of V.faba seedling showed significant difference in inductive concentration but no significant difference in culture time.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2012-05-25