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|本期目录/Table of Contents|

金线兰种子萌发及其原球茎的增殖培养

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2010年04期
页码:
555-559
栏目:
植物生理学与分子生物学
出版日期:
2010-07-25

文章信息/Info

Title:
Seed germination and the protocorm multiplication of Anoectochilus roxburghii
作者:
邱玥12龚宁12*张奎一12
⒈贵州师范大学 生命科学学院, 贵阳 550001; ⒉贵州省山地环境信息系统与生态环境重点实验室, 贵阳 550001
Author(s):
QIU Yue12 GONG Ning12* ZHANG KuiYi12
1.College of Life Sciences, Guizhou Normal University, Guiyang 550001, China; 2.Guizhou Provincial Key Laboratory for Montain Environment, Guiyang 550001, China
关键词:
金线兰 种子萌发 原球茎 增殖
Keywords:
Anoectochilus roxburghii seed germination protocorm multiplication
分类号:
-
DOI:
-
文献标识码:
A
摘要:
研究了影响金线兰种子非共生萌发的因素,并应用正交试验研究基本培养基、6BA、ZT、NAA四种因素对原球茎增殖的作用。结果表明:授粉类型对金线兰种子非共生萌发影响较大,异株异花、同株异花以及同株同花授粉所得的种子的萌发率分别为78.53%、69.62%、39.87%;蒴果成熟度以生长150 d为宜,采收后萌发率可达78.59%;冷藏影响种子的活力,种子的萌发率随冷藏时间的延长而降低;使用次氯酸钠浸泡后的种子与对照相比,其萌发率无明显差异;NAA对原球茎增殖作用显著,适宜于原球茎增殖的培养基为1/2MS+ZT0.5mg/L+NAA1.0mg/L。
Abstract:
Seed germination of Anoectochilus roxburghii in asymbiotie culture and the effects of four factors(culture medium,6BA,ZT and NAA)on protocorm multiplication through orthogonal design were studied. The results showed that pollination type was an important factor on A.roxburghii seeds' nonsymbiotic germination,germination ratio of the xenogamy seeds reached 78.53%,and the germination ratio of the geitonogamy seeds reached 69.62%,while the germination ratio of selfpollination was only 39.87%. The most suitable maturity of capsule appeared when it grew for 150 days,the germination rate of such seeds could reach 78.59%. The vitality of the seeds were affected by refrigerated storage:the germination rate of the seeds was decreased with the increase of seed cool storage time. There were no significant changes of seed germination rate between seed treatment by 10% NaClO solution and the control. NAA had a significant effect on the proliferation of protocorm,the optimum culture medium for protocorm proliferation was 1/2MS+ZT0.5mg/L+NAA1.0mg/L.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2010-07-01