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|本期目录/Table of Contents|

水稻籼爪重组自交系PEG胁迫早期基因差异表达分析

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2010年04期
页码:
538-543
栏目:
植物生理学与分子生物学
出版日期:
2010-07-25

文章信息/Info

Title:
Differentially expressed gene of indica/javanica recombinant inbred line in rice at early stage of PEG stress
作者:
樊庆鲁12肖国樱1*
1.中国科学院 亚热带农业生态研究所, 长沙 410125; 2.中国科学院 研究生院, 北京 100049
Author(s):
FAN QingLu12 XIAO GuoYing1*
1.Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; 2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
水稻 籼爪重组自交系 PEG 干旱胁迫 差异表达基因
Keywords:
rice indica/javanica recombinant inbred line PEG drought stress differentially expressed gene
分类号:
-
DOI:
-
文献标识码:
A
摘要:
把从籼爪重组自交系中筛选出的最耐旱材料(47274)和最敏感材料(47299)在五叶一心时用20% PEG处理6 h,取叶片进行mRNA差异显示分析,获得10个差异表达的cDNA片段。利用反式Northern斑点杂交法去除6个假阳性片段,对4个阳性片段(DTDF1、DTDF2、DTDF3、DTDF4)测序后进行同源性分析,发现DTDF3的蛋白产物与核酸结合位点和富亮氨酸重复结构域类蛋白B高度同源,DTDF2与L乳酸氧化酶部分序列有同源性,DTDF1没有找到同源蛋白质序列,DTDF4与一个假设蛋白高度同源。
Abstract:
The drought tolerant line and drought sensitive line screened from the population of indica/javanica recombinant inbred line were stressed with 20% PEG(Polyethyleneglycol) for 6 h at fiveleaf stage of rice. Ten of cDNA fragments were isolated by mRNA differentially display reverse transcription PCR(DDRTPCR),but only four fragments were approved truth by reverse Northern dot blotting. These fragments named as DTDF1,DTDF2,DTDF3 and DTDF4 were sequenced and then analyzed by Blast program. The putative protein of DTDF3 was highly homologous with NBSLRRlike protein B and that of DTDF2 was homologous with Llactate oxidase. The putative protein of DTDF1 was not found homologous protein,and that of DTDF4 was highly homologous with a hypothetical protein.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2010-07-01