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|本期目录/Table of Contents|

不同提取方法对黔产水香薷挥发性成分的影响

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2012年02期
页码:
269
栏目:
植物化学与化学生物学
出版日期:
2012-03-25

文章信息/Info

Title:
Effects of different extraction methods on volatile chemical constituents of the Elscholtzia kachinensis distributed in Guizhou Province
作者:
崔范洙1 王道平2 杨再昌2 林聪明2 马琳2 杨小生12*
1.贵阳中医学院, 贵阳 550001; 2.贵州省、中科院天然产物化学重点实验室, 贵阳 550002
Author(s):
CUI FanZhu1 WANG DaoPing2 YANG ZaiChang2 LIN CongMing2 MA Lin2 YANG XiaoSheng12*
1.Guiyang College of Traditional Chinese Medicine, Guiyang 550001, China; 2.Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China
关键词:
水香薷 挥发性化学成分 水蒸气蒸馏法 固相微萃取法体外抗菌试验
Keywords:
Elscholtzia kachinensis volatile chemical constituents steam distillation solidphase microextrations antimicrobial assay in vitro
分类号:
R932
DOI:
-
文献标识码:
A
摘要:
比较不同提取方法对黔产水香薷挥发性成分影响。采用固相微萃取法、水蒸气蒸馏法提取水香薷挥发性成分,用GCMS联用仪进行测定,结合NIST05和WILEY275谱库鉴定各化合物,峰面积归一化法测定各成分相对含量。利用两种提取方法供从水香薷中鉴别出67个化合物。从固相微萃取法提取物中检测并鉴定了45个化合物,而从水蒸气蒸馏法提取物种检测并鉴定了55个化合物。其中挥发性相同的化合物有33种,两种不同提取方法得到的挥发性成分虽有差异,但主要成分变化不大。体外抗菌试验表明,水香薷挥发性成分具有抑菌作用。这些研究结果为香料植物水香薷的开发提供了科学依据。
Abstract:
For analysizing and comparing the difference of the volatile chemical constituents of Elscholtzia kachinensis distributed in Guizhou Province, different extraction methods were used, including steam distillation and solidphase microextrations methods,gas chromatography and mass spectrometry(GC/MS)together with spectral banks(NIST05 and WILEY275) to analysize and identify constituents,and area normalization method to determine the relative content of each constituent. Sixtyseven components were identified and 33 compounds were coexisted in the two kinds of extractions. 45 compounds were found in the solidphase microextrations,55 compounds were observed in the steam distillation extraction. There was not remarkable difference of volatile components checked in the E.kachinensis between the two extraction methods. The volatile components showed antimicrobial activities in vitro. The results provided the scientific evidences for exploitation of spiceberry E.kachinensis.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2012-03-25