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韭葱黄条病毒、洋葱黄矮病毒和胡葱黄条病毒六安分离物CP基因克隆及同源性分析

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2012年02期
页码:
231
栏目:
植物生理学与分子生物学
出版日期:
2012-03-25

文章信息/Info

Title:
CP gene cloning and sequence analysis of Liu’an isolates of Leek yellow stripe virus, Onion yellow dwarf virus and Shallot yellow stripe virus
作者:
韦传宝12* 华俊雅1 杨宇1 隗洋洋1 史利利1
1.皖西学院 生物与制药工程学院, 安徽 六安 237012; 2.安徽省植物生物技术实训中心, 安徽 六安 237012
Author(s):
WEI ChuanBao12* HUA JunYa1 YANG Yu1 WEI YangYang1 SHI LiLi1
1.College of Biological and Pharmaceutical Engineering, Western Anhui University, Liu’an 237012, China; 2.Plant Biotechnological Training Center of Anhui Province, Liu’an 237012, China
关键词:
韭葱黄条病毒 洋葱黄矮病毒 胡葱黄条病毒 CP基因 序列分析
Keywords:
Leek yellow stripe virus Onion yellow dwarf virus Shallot yellow stripe virus CP gene sequence analysis
分类号:
S436.421
DOI:
-
文献标识码:
A
摘要:
设计特异性引物PCR扩增了六安大蒜病样中的韭葱黄条病毒(Leek yellow stripe virus,LYSV)、洋葱黄矮病毒(Onion yellow dwarf virus,OYDV)和胡葱黄条病毒(Shallot yellow stripe virus,SYSV)的全长CP基因,插入到pGEMT载体并测序。分别比较3种病毒CP基因种内变异性和种间亲缘关系。结果表明LYSV六安分离物CP基因由864个碱基组成,与Genbank上已报道的68个LYSV不同分离物CP基因的核苷酸序列同源性为76.12%~84.31%;OYDV的CP基因由771个碱基组成,与Genbank上已报道的86个OYDV不同分离物同源性为81.06%~90.40%;SYSV的CP基因由774个碱基组成,与Genbank上已报道的11个SYSV不同分离物CP基因同源性为88.63%~94.32%;从分析结果来看,LYSV的CP基因不同分离物之间变异性较大,OYDV CP变异性不大,SYSV变异性很小;3种病毒都有1个以上的宿主,病毒种内不同宿主分离物之间CP序列差异很小。进化分析显示OYDV和SYSV的CP基因亲缘性较近并成簇,LYSV的CP基因与OYDV和LYSV的CP基因亲缘性较远。
Abstract:
Specific primers were designed to amplify CP genes of Leek yellow stripe virus(LYSV),Onion yellow dwarf virus(OYDV)and Shallot yellow stripe virus(SYSV)Liu’an isolates. Then the CP genes were cloned into pGEMT vectors and were sequenced. Sequence analysis indicated that CP genes of LYSV,OYDV and SYSV Liu’an isolates consists of 864,771 and 774 nucleotide acids respectively. Multiple aligments showed that CP gene of LYSV Liu’an isolate shared 76.12%-84.31% nucleotide acids identities with 68 LYSV CP genes reported on Genbank,CP gene of OYDV Liu’an isolate shared 81.06%-90.40% nucleotide acids identities with 86 OYDV CP genes reported on Genbank and CP gene of SYSV Liu’an isolate shared 88.63%-94.32% nucleotide acids identities with 11 SYSV CP genes reported on Genbank. These results indicated that the divergence in LYSV CP among different isolates was high,whilst divergence of OYDV CP was moderate in different isolates. All the results indicated that the variability of LYSV CP gene among different isolates was high,OYDV CP gene among different isolates was in the middle and SYSV CP gene among different isolates was low. Phylogenetic analysis showed that LYSV CP had little relationship with OYDV CP and SYSV CP and OYDV had closer relationship with SYSV than LYSV.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2012-03-25