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|本期目录/Table of Contents|

联合应用ESI MS和1H NMR分析含笑属植物种子磷脂

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2012年01期
页码:
129
栏目:
植物化学与化学生物学
出版日期:
2012-01-25

文章信息/Info

Title:
Application of ESIMS combined with 1H NMR in analyzing seed phospolipids of six species of Michelia
作者:
唐安军1 Naomichi BAbA2
1.重庆市重庆师范大学生命科学学院, 重庆 401331, 中国; 2.日本冈山大学自然科学与技术研究生院, 冈山县津岛北 7008530, 日本
Author(s):
TANG AnJun1 Naomichi BABA2
1.College of Life Sciences, Chongqing Normal University, Chongqing 400047, China; 2.Graduate School of Natural Science and Technology, Okayama University, Tsushimanaka Okayama 7008530, Japan
关键词:
种子磷脂 质谱 核磁共振 指纹 含笑属
Keywords:
Seed phospholipid ESI MS NMR Fingerprinting Michelia
分类号:
Q945
DOI:
-
文献标识码:
A
摘要:
首次尝试利用电喷雾电离质谱(ESI MS)和氢原子核磁共振(1H NMR)技术分析了含笑属六种植物种子的磷脂特性,发现在两个指纹图谱区发现明显的差异,即在质荷比(m/z)895910(ESI MS)和5.30~540 mg/L(1H NMR)两个特异的区域存在显著差异。这些源于种子磷脂ESI/MS和1H NMR的谱带差异可以被用来分析不同植物的种子的磷脂特征。而且,相似地,在更广的层面上,特异性的谱带差异可用于分析其他植物种子的磷脂组成和特性,辅助鉴定种子。
Abstract:
To date,there was no example to authenticate seed phospholipids based on their fingerprinting. By means of electrospray ionization mass spectrometry(ESI MS)and proton nuclear magnetic resonance(1H NMR)spectroscopy,phospholipids fraction extracted from seeds of six species from the genus Michelia were detected. It was firstly found that distinct difference in spectral fingerprinting region between m/z 895910 in ESI MS and 5.30-5.40 mg/L in 1H NMR among these six species. Thus,it suggested that the spectral differences shown by ESI MS and 1H NMR among seeds of these six species could be applied to identify them. And in a wider sense,for analyzing seed phospholipids of other plant species,a combination of ESI MS AND NMR was an effective tool.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2012-01-25