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|本期目录/Table of Contents|

尾巨桉HMGR基因的克隆及表达分析

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2012年01期
页码:
113
栏目:
植物生理学与分子生物学
出版日期:
2012-01-25

文章信息/Info

Title:
Molecular Cloning and expression analysis of 3hydroxy3methylglutarylCoA Reductase Gene from Eucalyptus urophylla×E.grandis
作者:
蒋春1 张华玲2 彭江2*
1.四川大学 生命科学学院 生物资源与生态环境教育部重点实验室, 四川 成都 610064; 2.西南大学 生命科学学院 三峡库区生态环境教育部重点实验室, 重庆 400715
Author(s):
JIANG Chun1 ZHANG HuaLing2 PENG Jiang2*
1.Key Laboratory of Bioresources and Ecoenvironment of Ministry of Education, School of Life Sciences, Sichuan University, Chengdu 610064, China; 2.Key Laboratory of Ecoenvironments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China
关键词:
尾巨桉 HMGR 生物信息学 内含子 组织特异性表达
Keywords:
Eucalyptus urophyla×E.grandis HMGR bioinformatics intron tissue specific expression
分类号:
Q943.2;Q946.85
DOI:
-
文献标识码:
A
摘要:
基于RACE技术克隆得到尾巨桉3羟基3甲基戊二酰CoA还原酶(EuHMGR)基因全长为1 955 bp,包含1 560 bp的开放阅读框(ORF),编码519个氨基酸。生物信息学分析表明EuHMGR包含两个HMGCoA结合基序和两个NADP(H)结合基序;同源建模得到EuHMGR三维构象呈“V”型,包含N结构域、S结构域和L结构域。将EuHMGR组DNA与cDNA序列进行比对表明EuHMGR存在一个319 bp的内含子。通过实时定量PCR进行组织特异性表达分析表明EuHMGR在茎中的表达量最高,叶次之,在根中基本无表达。该研究为后续尾巨桉EuHMGR的功能验证以及遗传转化奠定基础。
Abstract:
The gene encoding 3hydroxy3methylglutarylCoA reductase (HMGR)was cloned by Rapid Amplification of cDNA Ends (RACE)method,the fulllength EuHMGR cDNA was 1955 bp,containing a 1 560 bp open reading frame,encoding a peptide of 519 amino acids. Bioinformatics analysis indicated EuHMGR contained two HMGCoA binding motifs and two NADP(H)binding motifs;and it had a 3D structure with“V”homologybased on modeling,containing Ndomain,Sdomain and Ldomain. There was a 319 bp intron in EuHMGR gene genomic DNA sequence compared with the cDNA sequence. Tissue expression profile analysis by RealTime quantitative PCR indicated EuHMGR expression was the highest in stem,followed by leaf 〖JP3〗and no expression in root. The paper would supply some information to the function analysis and genetic transformation of EuHMGR from Eucalyptus urophylla×E.grandis.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2012-01-25