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|本期目录/Table of Contents|

栲树叶片SEM样品制备

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2012年01期
页码:
53
栏目:
出版日期:
2012-01-25

文章信息/Info

Title:
SEM sample preparation for leaf epidermis of Castanopsis fargesii
作者:
胡华强12 胡晓颖1 孙晔13*
1.中国科学院 华南植物园, 广州 510650; 2.中国科学院 研究生院, 北京 100049; 3.中国科学院 华南植物园植物资源保护与可持续利用重点实验室, 广州 510650
Author(s):
HU HuaQiang12 HU XiaoYing1 SUN Ye13*
1.South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650; 2.Graduate University of Chinese Academy of Sciences, Beijing 100049; 3.Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
关键词:
栲树叶片 薄壁盾状毛 SEM 样品制备
Keywords:
leaf epidermis thinwalled peltate trichomes Scanning Electronic Microscope sample preparation
分类号:
Q336
DOI:
-
文献标识码:
A
摘要:
: 栲树叶片下表皮覆盖着一层密集的薄壁盾状毛影响了对其叶表面特征的观察。经FAA固定→二甲苯和丙酮的混合液(1∶1)浸泡→恒温金属浴加温→超声波(50~60 Hz,90 W)振荡器→系列酒精浓度脱水处理后,叶表面薄壁盾状毛脱落,在SEM扫描电子显微镜下可观察到叶表皮形态结构(气孔器,细胞排列及近圆形的毛基)。此方法操作简便、效果明显,并且安全和无污染。
Abstract:
The lower epidermis of the leaves of Castanopsis fargesii(Fagaceae) covered densely with thinwalled peltate trichomes gave rise to difficulty for the observation of its micromorphological characteristics. The thinwalled peltate trichome was shed off when the leaves were treated with FAA solution and a mixture of dimethylbenzene and acetone(1∶1),followed by shaking in an ultrasonic wave bath(50-60 Hz,90 W)and rinsing with a series of ethanol concentration. The micromorphological characteristics of leaf epidermis could be shown clearly under the scanning electronic microscope(SEM). This method was very effective and easy to operate,furthermore,it was safe and nonpolluted.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2012-01-25