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|本期目录/Table of Contents|

天然产物莪术醇单克隆抗体的制备

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2011年06期
页码:
832
栏目:
植物化学与化学生物学
出版日期:
2011-12-25

文章信息/Info

Title:
Formation of monoclonal antibody against a major natural product curcumol
作者:
陈旭12 钟海雁1* 王娟2 曾建红12
1.中南林业科技大学, 长沙 410004; 2.桂林医学院 药学院, 广西 桂林 541004
Author(s):
CHEN Xu12 ZHONG HaiYan1* Wang Juan2 ZENG JianHong12
1.Central South University of Forestry and Technology, Changsha 410004, China; 2.Guilin Pharmaceutical School, Guilin 541004, China
关键词:
天然产物 莪术醇 单克隆抗体 制备
Keywords:
natural product curcumol monoclonal antibody preparation
分类号:
R932
DOI:
-
文献标识码:
摘要:
为制备小分子化合物莪术醇的单克隆抗体,先将莪术醇(curcumol)与载体蛋白牛血清蛋白(BSA)偶联形成完全抗原,用基质辅助激光解吸飞行时间质谱法(MALDITOFMS)鉴定莪术醇人工抗原的偶联率,然后采用杂交瘤技术获得杂交瘤株,并对其进行小鼠腹水的制备与纯化。结果表明:莪术醇半抗原与载体的偶联比为196,单克隆抗体的腹水效价为1∶51 200,获得的单克隆抗体只与莪术醇抗原发生特异反应,聚丙烯酰胺凝胶电泳((SDSPAGE)显示,单克隆抗体重链的分子量为5 000,轻链的分子量为2 500。得到了莪术醇单克隆抗体,为利用免疫分析技术对莪术类药材进行质量控制与检测奠定了理论基础。
Abstract:
To get monoclonal antibody(mAb)against crude drug curcumol,curcumol carrier protein conjugates were synthesized by the method that curcumol was conjugated with BSA. The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrixassisted laser desorption/ionization mass spectrometry〖JP3〗(MALDITOFMS). A hybridoma secreting monoclonal antibody against curcumol was produced by hybridoma technology,then preparation and purification of curcumol mAb use mice hybridoma ascitic fluid. Results showed that the ratio of hapten and bovine serum album was 19.6,the ELISA titers in the ascite fluids of the curcumol mAb were 1∶51 200,ELISA analysis also proved that the curcumol mAbs reacted specifically with curcuoml antigen,polyacrylamide gel electrophoresis((SDSPAGE)assay showed that the curcumol heavy chain was 5.0×104 and the light chain was 2.5×104. Conclusion the specific anticurcumol mAbs were prepared,which would lay a theoretical foundation for detection and qulity control and immunoassay of curcuma kwangsiensis in Guangxi producing areas by Immunoassay.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-11-25