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|本期目录/Table of Contents|

蛇苔过氧化物酶基因的序列分析与功能预测

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2011年06期
页码:
806
栏目:
植物生理学与分子生物学
出版日期:
2011-12-25

文章信息/Info

Title:
Sequence analysis and function predication of poroxidase from Conocephalum conicum
作者:
李茹兰12 边春象2 阮期平2* 彭正松1 姜立春2 刘美2
1.西华师范大学 生命科学学院, 南充 637000; 2.绵阳师范学院 分子生物学与生物制药重点实验室, 绵阳 621000
Author(s):
LI RuLan12 BIAN ChunXiang2 RUAN QiPing2* PENG ZhengSong1 JIANG LiChun2 LIU Mei2
1.College of Life Sciences, China West Normal University, Nanchong, Sichuan 637000, China; 2.Key Laboratory for Molecular Biology and Biopharm aceuticals, Mianyang Normal University, Mianyang 621000, China
关键词:
过氧化物酶 蛇苔 序列分析 功能预测
Keywords:
peroxidases Conocephalum conicum sequence analysis function predication
分类号:
Q943
DOI:
-
文献标识码:
A
摘要:
过氧化物酶在生物界分布极广,在细胞代谢的氧化还原过程中起重要的作用。为深入研究蛇苔过氧化物酶的理化性质及功能,对其同源性,亚细胞定位,结构和功能等进行了生物信息学预测和分析。结果表明:该序列有1 050 bp长的开放阅读框,编码349个氨基酸,与拟南芥等的POD相似性较高(相似性为71%)。预测R68、F71、H72、P170、H200和D276为该酶的活性位点,C41与C122、C74与C79、C128与C328、C207与C239形成四对二硫键,对蛋白质的功能、天然构象和稳定性有重要作用,为研究苔类植物过氧化物酶的作用机制和功能等奠定了基础。
Abstract:
Peroxidases is widespread in biological world. It plays an important role in the cell redox metabolism.In order to furher study the characteristics and functions of Conocephalum conicum poroxidase,we performed bioinformatics analysis of its homology,subellular location,tertiary structure,function and so on. The results showed that the sequence had a complete open read frame of about 1 050 bp and encodes 349 amino acids,and the sequence had similar POD with that of Arabidopsis thaliana(the similarity beween them is 71%). This sequence was predicated to be C.conicum poroxidase which belonged to the poroxidase super family,and R68、F71、H72、P170、H200 and D276 to be the C.conicum POD’s active sites in which the four groups of disulfide bonds:C4 and C122、C74 and C79、C128 and C328、C207 and C239 played a very important role in maintaining the protein’s function,natural conformation and stability. This study would set an important foundation for the study of the interaction mechanisms and functions of the C.conicum poroxidase.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-11-25