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|本期目录/Table of Contents|

采用高效液相色谱技术分析烟草体内的维生素B6化合物

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2011年05期
页码:
695
栏目:
植物化学与化学生物学
出版日期:
2011-09-25

文章信息/Info

Title:
Analysis of vitamin B6 vitamers in tobacco plants by high performance liquid chromatography
作者:
曾海彬1 张剑韵2 黄龙全1*
1.安徽农业大学 茶与食品科技学院, 合肥 230036; 2.安徽农业大学 生命科学学院, 合肥 230036
Author(s):
ZENG HaiBin1 ZHANG JianYun2 HUANG LongQuan1*
1.College of Tea and Food Science and Technology, Anhui Agricultural University,  Hefei 230036, China; 2.College of Life Sciences, Anhui Agricultural University,  Hefei 230036, China
关键词:
烟草 高效液相色谱 维生素B6 存在形态
Keywords:
tobacco plants high performance liquid chromatography vitamin B6 existing forms
分类号:
Q945.14
DOI:
-
文献标识码:
A
摘要:
维生素B6(VB6)是一类化合物的总称。近年来研究发现VB6在植物体内发挥抗逆作用。烟草作为模式植物其体内VB6的存在形态还未见报道。本研究采用高效液相色谱结合荧光检测技术对烟草体内VB6的存在形态进行了分析。结果表明:土壤栽培烟草叶、茎和根中VB6的含量依次为2.9、1.7、3.0 μg/g鲜重;组培烟草叶片的VB6含量为3.9 μg/g鲜重,构成比为磷酸吡哆胺(PMP)7%、吡哆胺(PM)14%、磷酸吡哆醛(PLP)19%、吡哆醛(PL)29%、吡哆醇(PN)30%;组培烟草在连续3周的检测过程中,PLP和PL含量下降、PN含量上升,VB6总量保持相对稳定。研究结果有助于以烟草为材料,进一步开展植物体内VB6代谢机制和特殊生理机制的研究。
Abstract:
Vitamin B6(VB6)is the general term for a kind of chemical compounds .VB6 exists in several forms,and has been linked to stress responses in plants. Until now no reports about the distribution of B6 vitamers in tobacco plants have been observed. In our experiment,the determination of VB6 vitamers in tobacco plants was described by using HPLC with fluorescence detector. The results indicated that,the contents of VB6 in leaves,tender stem and roots were 2.9,1.7 and 3.0 μg/g fresh weight,respectively. Leaves of tobacco plants grown on MS basal media exhibited a high content of 3.9 μg/g fresh weight. The constituent ratio of B6 vitamers were as follows: pyridoxamine 5′phosphate(PMP) 7%,pyridoxamine(PM) 14%,pyridoxal 5′phosphate(PLP) 19%,pyridoxal(PL) 29% and pyridoxine(PN) 30%. During the determination period of three weeks,the contents of PLP and PL decreased,and that of PN increased. The amount of VB6 was relatively constant. Our results would be favorable for further study on the metabolic mechanism and special physiological mechanism in tobacco plants.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-09-25