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高温胁迫下五种杜鹃花属植物的生理变化及其耐热性比较

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2011年05期
页码:
651
栏目:
植物生理学与分子生物学
出版日期:
2011-09-25

文章信息/Info

Title:
Physiological changes and heat tolerance comparison of five Rhododendron species under hightemperature stress
作者:
张乐华1 孙宝腾2 周广12 王书胜1 李晓花1 单文1
1.江西省、中国科学院 庐山植物园, 江西 庐山 332900; 2.南昌大学 生命科学与食品工程学院, 南昌 330031
Author(s):
ZHANG LeHua1 SUN BaoTeng2 ZHOU Guang12 WANG ShuSheng1 LI XiaoHua1 SHAN Wen1
1.Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences, Lushan 32900, China; 2.College of Life Science and Food Engineering, Nanchang University, Nanchang 330031, China
关键词:
杜鹃花属 高温胁迫 生理生化指标 耐热性 隶属函数 聚类分析
Keywords:
Rhododendron hightemperature stress physiological and biochemical index heat tolerance subordinate function cluster analysis
分类号:
Q945.78
DOI:
-
文献标识码:
A
摘要:
高温是制约分布于较高海拔地区杜鹃花迁地保育与园林应用的重要因子。为探讨杜鹃花属植物的高温致伤机理,该实验以隶属不同亚属的白花杜鹃、羊踯躅、毛棉杜鹃、红滩杜鹃及红棕杜鹃4年生实生苗为材料,通过人工气候箱的盆栽实验,研究了30 ℃、38 ℃高温胁迫下其叶片生理生化指标的变化,并利用隶属函数法及系统聚类分析法对其种间耐热性进行了综合评价。结果表明:高温胁迫下,5种杜鹃叶片的过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性及脯氨酸(Pro)、过氧化氢(H2O2)、丙二醛(MDA)含量均随着胁迫温度的升高而增大;超氧化物歧化酶(SOD)活性在30 ℃轻度胁迫时均小幅度上升,而38 ℃重度胁迫时下降。2个高温条件下,SOD、CAT的变化幅度与杜鹃种间耐热性总体呈正相关,而H2O2、MDA增幅与种间耐热性呈负相关;Pro增幅在30 ℃轻度胁迫时与种间耐热性呈负相关,而38 ℃重度胁迫时与耐热性呈正相关;APX增幅与种间耐热性的相关性较弱。隶属函数与聚类分析综合评判得出5种杜鹃耐热性强弱顺序为:白花杜鹃>羊踯躅、毛棉杜鹃>红滩杜鹃>红棕杜鹃,与其田间耐热性表现一致。
Abstract:
High temperature is the important constraining factor of Rhododendron in ex situ conservation and landscape application. In order to reveal the injury of mechanism of five Rhododendron species under hightemperature stress,R.mucronatum,R.molle,R.moulmainense,R.chihsinianum and R.rubiginosum which belong to different subg.Rhododendron were used in the experiment. Four yearsold seedlings of these five species were planted in artificial climate chambers and their changes of physiological and biochemical index were determined under hightemperature of 30 ℃ and 38 ℃ stress,and the heat tolerance was comprehensively evaluated with fuzzy subordinate function and system cluster analysis. The results showed that the activities of catalase(CAT),ascorbate peroxidase(APX)in leaves of five species were increased with temperature becoming higher,so do the contents of proline(Pro),hydrogen peroxide (H2O2) and malondialdeyde(MDA). The activity of superoxide dismutase(SOD)increased slightly under 30 ℃ and decreased under 38 ℃. The variational extent of SOD and CAT had positive correlation with the heat tolerance and the increase extent of H2O2 and MDA had the negative correlation under high temperature. The increase extent of Pro had negative correlation with the heat tolerance under 30 ℃ and positive correlation under 38 ℃. There was weak correlation between the increase extent of APX and the heat tolerance. The comprehensive evaluation of subordinate function and cluster analysis indicate that the heat tolerance sequence of five Rhododendron species was R.mucronatum>R.molle and R.moulmainense>R.chihsinianum>R.rubiginosum,in accordance with their behavior in the field.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-09-25