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应用RAPD和ISSR标记对24份花生栽培种材料进行遗传多样性分析

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2011年05期
页码:
584
栏目:
系统与演化植物学
出版日期:
2011-09-25

文章信息/Info

Title:
Genetic diversity in 24 peanut cultivars as revealed by RAPD/ISSR profiling
作者:
闫苗苗1 魏光成1* 谭秀华1 王传堂2
1.滨州医学院, 山东 烟台 264003; 2.山东省花生研究所, 山东 青岛 266100
Author(s):
YAN MiaoMaio1 WEI GuangCheng1* TAN XiuHua1 WANG ChuanTang2
1.Binzhou Medical College, Yantai 264003, China; 2.Shandong Peanut Research Institute, Qingdao 266100, China
关键词:
花生 RAPD ISSR 聚类分析 遗传多样性
Keywords:
peanut RAPD ISSR cluster analysis genetic diversity
分类号:
Q943
DOI:
-
文献标识码:
A
摘要:
利用RAPD引物和ISSR引物分析我国24份花生栽培种材料的遗传多样性。结果表明:所选的RAPD引物和ISSR引物中分别有13条引物和10条引物扩增出了清晰并可重复的条带,共扩增出123条带和87条带,平均每条引物扩增出9.5条带和8.7条带,其中多态性带分别占条带总数的47.15%和57.47%,平均每条引物扩增出4.7条和5.7条多态性带。在此基础上,根据NeiLi系数采用UPGMA法进行了聚类分析,可将24份花生材料分成4类:四粒红、汕油523、冀花2号、花育16和粤油7号等5个品种聚为一类;花育20、中花8号聚为一类;黑花生单独为一类;其余的花生聚为一类。RAPD和ISSR标记能够揭示花生栽培种的遗传多样性,在种质鉴定和遗传作图等方面具有一定应用潜力。
Abstract:
Genetic diversity in 24 accessions of cultivated peanut materials from China was evaluated based on RAPD/ISSR profiling. Of the RAPD primers and ISSR primers tested,13 and 10 primers produced a total of 123 and 87 repeatable,clear and readable bands,among which 47.15% and 57.47% were polymorphic,respectively. On average,a primer resulted in 9.5 and 8.7 bands,of which 4.7 and 5.7 were polymorphic. A dendrogram was constructed using UPGMA algorithm based on Nei and Li’s similarity coefficient,which divided the 24 peanut materials into 4 groups. Silihong,Sanyou 523,Jihua 2,Huayu 16 and Yueyou 7 were in one group. Huyu 20 and Zhonghua 8 clustered together. Heihuasheng formed a separated group,and the rest peanut materials tested fell in a group. RAPD and ISSR are useful for the genetic diversity studies of the cultivated peanut,and have potential in characterization of peanut germplasm and gene mapping.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-09-25