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|本期目录/Table of Contents|

姜花属种间杂交胚拯救研究

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2011年04期
页码:
541
栏目:
植物生理学与分子生物学
出版日期:
2011-07-25

文章信息/Info

Title:
Study on embryo rescue of interspecific hybrids between Hedychium
作者:
熊友华12 庄雪影2 刘念1*
1.仲恺农业工程学院 园艺园林学院, 广州 510225; 2.华南农业大学 林学院, 广州 510642
Author(s):
XIONG YouHua12 ZHUANG XueYing2 LIU Nian1*
1.College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; 2.College of Forestry, South China Agricultural University, Guangzhou 510642, China
关键词:
姜花属 种间杂交 胚拯救
Keywords:
Hedychium interspecific hybridization embryo rescue
分类号:
Q943.1
DOI:
-
文献标识码:
A
摘要:
为提高姜花属种间杂交胚挽救中幼胚萌发率,以白姜花×金姜花的胚珠为试材,研究不同胚珠发育时期、不同培养基及低温处理果实对幼胚萌发率的影响。结果表明,白姜花×金姜花胚挽救的适宜培养基是MS+0.1 mg/L BA+0.1 mg/L NAA;接种时期以60 d的幼胚培养效果最佳;低温处理果实3~6 d能有效提高幼胚的萌发率。
Abstract:
The object of this study was to improve the germination capacity of embryoes of Hedychium hybrids through embryo rescue in vitro. Hybrid ovules of H.coronarium×H.gardnerianum were used to investigate the effect of different growth stage of embryo,different culture medium and lowtemperature treatment on the germination capacity of embryoes. The results indicated that MS medium supplemented with 0.1 mg/L BA and 0.1 mg/L NAA showed suitable for embryo rescue in vitro culture. It was showed that the optimum germination capacity of embryoes reached 40.6%,which was obtained by taking 60 day old immature embryo as explant. Lowtemperature treatment of fruits for 3-6 d,had a positive effect on improvement of the germination capacity of embryoes.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-07-25