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|本期目录/Table of Contents|

姜荷花炭疽病病原菌分离与鉴定

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2011年04期
页码:
531
栏目:
植物生理学与分子生物学
出版日期:
2011-07-25

文章信息/Info

Title:
Isolation and identification of pathogens that caused Curcuma alismatifolia anthracnose
作者:
赵健1 赵志国1 李秀娟1 张翠萍1 区蝉1 林萌2 仇硕1*
1.广西壮族自治区中国科学院广西植物研究所, 广西 桂林 541006; 2.桂林市石山绿化试验站, 广西 桂林 541001
Author(s):
ZHAO Jian1ZHAO ZhiGuo1 LI XiuJuan1 ZHANG CuiPing1 OU Chan1 LIN Meng2 QIU Shuo1*
1.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China; 2.Limestone Greening Station of Guilin, Guilin 541001, China
关键词:
姜荷花 炭疽病 分离 鉴定
Keywords:
Curcuma alismatifolia anthracnose isolation identification
分类号:
S432
DOI:
-
文献标识码:
A
摘要:
利用常规组织分离法对姜荷花炭疽病病原菌进行了分离,并对分离菌株进行培养、纯化、回接和重新分离,最后利用形态学和分子生物学技术对致病菌株进行了鉴定。结果表明:从感病叶片中分离得到6株病原菌,病原菌室外回接发现只有菌株Cum3致病,Cum3室外回接致病率96.7%,室内回接致病率100%。形态学鉴定,Cum3为镰孢酶属病原菌,菌落圆型,菌丝白色,有隔膜,有分枝;分生孢子有小型、大型和厚垣3种。对菌株Cum3进行ITS序列分析,获得全长528 bp的序列,该序列与Genbank中已登陆的序列进行相似性分析,并结合田间致病症状,认为该致病菌为层出镰孢菌。
Abstract:
The pathogens that caused Curcuma alismatifolia anthracnose were isolated using usual tissue isolation. Strains were cultivated,purified,reinoculated in the field and laboratory. The strain that caused the anthracnose was reisolated. Morphological and molecular biological techniques were used to identify the strain. Results showed that 6 strains were isolated from infected leaves of C.alismatifolia,but only Cum3 caused the anthracnose. The incidences of Cum3 disease reinoculated in the field and laboratory were 96.7% and 100% respectively. Cum3 is a kind of fungi belonging to the genus Fusarium,and its colonial morphology is a circular form,with white mycelium,diaphragms and branches. There are three kinds of conidia:microconidia,macroconidia and chlamydospores. At last,the sequence of internal transcribed spacer (ITS) region of Cum3 was analyzed,the length was 528bp and the sequence was compared with other species in the Genbank. The symptom in the field indicates that the pathogen of Curcuma alismatifolia anthracnose is Fusarium proliferatum.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2011-07-25